Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   QSG83_RS03845 Genome accession   NZ_CP127892
Coordinates   585469..586257 (+) Length   262 a.a.
NCBI ID   WP_371971077.1    Uniprot ID   -
Organism   Acinetobacter towneri strain SAAt401     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 580469..591257
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QSG83_RS03820 (QSG83_03820) - 580910..582193 (+) 1284 WP_104852475.1 glycosyltransferase family 1 protein -
  QSG83_RS03825 (QSG83_03825) - 582219..582770 (+) 552 WP_004975087.1 phosphatase PAP2 family protein -
  QSG83_RS03830 (QSG83_03830) iscX 583230..583430 (+) 201 WP_104852473.1 Fe-S cluster assembly protein IscX -
  QSG83_RS03835 (QSG83_03835) ndk 583593..584024 (+) 432 WP_004975092.1 nucleoside-diphosphate kinase -
  QSG83_RS03840 (QSG83_03840) rlmN 584161..585393 (+) 1233 WP_004975094.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  QSG83_RS03845 (QSG83_03845) pilF 585469..586257 (+) 789 WP_371971077.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  QSG83_RS03850 (QSG83_03850) - 586248..587057 (+) 810 WP_225555761.1 helix-turn-helix domain-containing protein -
  QSG83_RS03855 (QSG83_03855) ispG 587080..588201 (+) 1122 WP_004975100.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  QSG83_RS03860 (QSG83_03860) hisS 588205..589500 (+) 1296 WP_253132228.1 histidine--tRNA ligase -
  QSG83_RS03865 (QSG83_03865) - 589531..590232 (+) 702 WP_131260757.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 262 a.a.        Molecular weight: 29451.33 Da        Isoelectric Point: 8.7703

>NTDB_id=845088 QSG83_RS03845 WP_371971077.1 585469..586257(+) (pilF) [Acinetobacter towneri strain SAAt401]
MAQITTVCISAIFAAACQTTAPTLGQKDPEKAVQVRTQLAAEYLKNGDLDSAKRALDQALESDSRDSTANMMMGVLLQQE
GSKLNLEKADAYFKRAIAADPKNAQARNNYGTYLYQVERYNDAIEQLSIAGATLGYDQRFRALENVGRIYLKLGDIENAE
KSFKQALQANRDSYVSMLELSEILYLRQQFAPARQLYEQFVRAVGQKNQGARALWIGIRLARANGDQLGTQVLVNQLRAL
FPNSQEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 789 bp        

>NTDB_id=845088 QSG83_RS03845 WP_371971077.1 585469..586257(+) (pilF) [Acinetobacter towneri strain SAAt401]
ATCGCACAAATTACAACTGTTTGTATTTCGGCAATTTTTGCTGCAGCTTGTCAAACCACAGCTCCAACCTTAGGGCAAAA
AGACCCTGAAAAAGCGGTGCAAGTACGTACGCAGTTGGCAGCAGAATACTTAAAAAATGGCGATTTAGACTCAGCCAAAC
GCGCACTTGATCAAGCTTTGGAAAGCGATTCACGTGATTCCACTGCTAATATGATGATGGGCGTGTTGCTGCAACAAGAA
GGCAGCAAGCTGAATTTAGAAAAAGCCGATGCGTATTTTAAGCGTGCCATTGCCGCTGACCCAAAAAATGCACAAGCGCG
TAACAACTATGGTACGTATTTATATCAAGTAGAACGCTATAATGATGCGATTGAGCAGCTCAGTATTGCAGGTGCAACAC
TGGGTTATGATCAACGCTTTAGAGCATTGGAAAATGTAGGTCGTATTTACTTGAAATTAGGCGATATAGAGAATGCCGAA
AAGTCATTCAAACAAGCCTTGCAAGCCAATCGCGATTCTTATGTTTCAATGTTAGAGTTGTCGGAAATTTTATATTTAAG
ACAACAATTTGCACCCGCGCGACAATTGTATGAACAATTTGTACGTGCTGTGGGGCAAAAAAATCAGGGTGCGCGTGCCT
TATGGATTGGGATTCGTTTAGCACGTGCCAATGGCGATCAATTGGGAACTCAGGTTTTAGTGAACCAGCTGCGTGCATTA
TTTCCCAATAGCCAAGAATACCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(93-125)

Tetratricopeptide repeat (TPR_16)

(39-76)

Tetratricopeptide repeat (TPR_16)

(147-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

77.778

96.183

0.748
  pilF Acinetobacter baylyi ADP1

72.047

96.947

0.698