Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   QRD24_RS16630 Genome accession   NZ_CP127874
Coordinates   3233992..3234414 (+) Length   140 a.a.
NCBI ID   WP_013057949.1    Uniprot ID   A0A8D3X0F8
Organism   Priestia megaterium strain ELP1.24a     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3228992..3239414
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QRD24_RS16605 (QRD24_16605) - 3229323..3230156 (-) 834 WP_253351461.1 AraC family transcriptional regulator -
  QRD24_RS16610 (QRD24_16610) - 3230379..3231200 (+) 822 WP_221841065.1 histidine kinase -
  QRD24_RS16615 (QRD24_16615) - 3231201..3231842 (+) 642 WP_013083981.1 response regulator transcription factor -
  QRD24_RS16620 (QRD24_16620) - 3231978..3233357 (+) 1380 WP_286127539.1 PepSY domain-containing protein -
  QRD24_RS16625 (QRD24_16625) - 3233387..3233851 (+) 465 WP_286127540.1 FixH family protein -
  QRD24_RS16630 (QRD24_16630) nucA/comI 3233992..3234414 (+) 423 WP_013057949.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  QRD24_RS16635 (QRD24_16635) - 3234555..3234908 (+) 354 WP_225049451.1 DUF1516 family protein -
  QRD24_RS16640 (QRD24_16640) - 3234949..3235542 (-) 594 WP_028409296.1 hypothetical protein -
  QRD24_RS16645 (QRD24_16645) - 3235697..3236317 (-) 621 WP_286127541.1 TetR/AcrR family transcriptional regulator -
  QRD24_RS16650 (QRD24_16650) - 3236340..3237620 (-) 1281 WP_286127542.1 glycosyltransferase -
  QRD24_RS16655 (QRD24_16655) - 3237917..3238462 (+) 546 WP_221841060.1 GNAT family N-acetyltransferase -
  QRD24_RS16660 (QRD24_16660) - 3238611..3239096 (-) 486 WP_028409300.1 hypothetical protein -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 15329.17 Da        Isoelectric Point: 4.4990

>NTDB_id=845024 QRD24_RS16630 WP_013057949.1 3233992..3234414(+) (nucA/comI) [Priestia megaterium strain ELP1.24a]
MKKIVSGILILVLAVIVMIEGNPLEQNSSTNTSYDEVITFPADRYPETAAHIQHAIDKGKSAVCTIDRDGAEENRRESLK
GIPTKSGYDRDEFPMAFCEEGGSGADIEYVKPSDNRGAGSWISHQVDEFPDGTKVLIEVK

Nucleotide


Download         Length: 423 bp        

>NTDB_id=845024 QRD24_RS16630 WP_013057949.1 3233992..3234414(+) (nucA/comI) [Priestia megaterium strain ELP1.24a]
TTGAAAAAAATTGTTTCTGGTATTTTAATTTTAGTATTAGCCGTTATTGTTATGATTGAAGGAAATCCGTTAGAGCAGAA
CTCTTCAACGAATACCTCCTACGATGAAGTAATTACCTTCCCTGCTGATCGTTACCCTGAAACTGCAGCGCATATTCAAC
ATGCAATCGATAAAGGAAAATCGGCTGTTTGTACAATTGACCGAGACGGGGCTGAGGAAAACCGCCGTGAGTCTTTAAAA
GGTATTCCGACTAAAAGCGGCTATGACCGAGATGAGTTTCCGATGGCCTTTTGTGAAGAAGGCGGAAGCGGTGCTGATAT
TGAATATGTAAAGCCTTCAGACAACCGAGGCGCCGGGTCTTGGATCTCCCATCAAGTAGACGAGTTTCCTGACGGTACGA
AGGTTTTAATTGAAGTGAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(43-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

61.207

82.857

0.507