Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   V2199_RS23465 Genome accession   NZ_CP143899
Coordinates   5062624..5064117 (-) Length   497 a.a.
NCBI ID   WP_003094357.1    Uniprot ID   A0A0H2ZGR9
Organism   Pseudomonas aeruginosa strain PA26_WM3_03.23_ST27     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5057624..5069117
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V2199_RS23430 (V2199_23430) - 5058475..5058924 (+) 450 WP_003094369.1 hypothetical protein -
  V2199_RS23435 (V2199_23435) - 5058937..5059548 (+) 612 WP_003098862.1 superoxide dismutase -
  V2199_RS23440 (V2199_23440) - 5059567..5060499 (+) 933 WP_003120918.1 ZIP family metal transporter -
  V2199_RS23445 (V2199_23445) - 5060596..5060868 (-) 273 WP_003094362.1 HPr family phosphocarrier protein -
  V2199_RS23450 (V2199_23450) rapZ 5060898..5061758 (-) 861 WP_003094361.1 RNase adapter RapZ -
  V2199_RS23455 (V2199_23455) ptsN 5061760..5062224 (-) 465 WP_003098853.1 PTS IIA-like nitrogen regulatory protein PtsN -
  V2199_RS23460 (V2199_23460) hpf 5062238..5062546 (-) 309 WP_003094359.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  V2199_RS23465 (V2199_23465) rpoN/rpoN1 5062624..5064117 (-) 1494 WP_003094357.1 RNA polymerase factor sigma-54 Machinery gene
  V2199_RS23470 (V2199_23470) lptB 5064343..5065068 (-) 726 WP_003094355.1 LPS export ABC transporter ATP-binding protein -
  V2199_RS23475 (V2199_23475) lptA 5065068..5065595 (-) 528 WP_003120916.1 lipopolysaccharide transport periplasmic protein LptA -
  V2199_RS23480 (V2199_23480) lptC 5065582..5066154 (-) 573 WP_003094353.1 LPS export ABC transporter periplasmic protein LptC -
  V2199_RS23485 (V2199_23485) - 5066163..5066702 (-) 540 WP_003112742.1 KdsC family phosphatase -
  V2199_RS23490 (V2199_23490) kdsD 5066702..5067682 (-) 981 WP_003094347.1 arabinose-5-phosphate isomerase KdsD -
  V2199_RS23495 (V2199_23495) - 5068095..5068904 (+) 810 WP_003094343.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 56054.22 Da        Isoelectric Point: 4.4327

>NTDB_id=844907 V2199_RS23465 WP_003094357.1 5062624..5064117(-) (rpoN/rpoN1) [Pseudomonas aeruginosa strain PA26_WM3_03.23_ST27]
MKPSLVLKMGQQLTMTPQLQQAIRLLQLSTLDLQQEIQEALESNPMLERQEDGDDFDNSDPLADGAEQAASAPQESPLQE
SATPSVESLDDDQWSERIPSELPVDTAWEDIYQTSASSLPSNDDDEWDFTARTSSGESLHSHLLWQVNLAPMSDTDRMIA
VTIIDSINNDGYLEESLEEILAAIDPELDVELDEVEVVLRRIQQLEPAGIGARNLRECLLLQLRQLPSTTPWLNEALRLV
SDYLDLLGGRDYSQLMRRMKLKEDELRQVIELIQCLHPRPGSQIESSEAEYIVPDVIVRKDNERWLVELNQEAMPRLRVN
ATYAGMVRRADSSADNTFMRNQLQEARWFIKSLQSRNETLMKVATQIVEHQRGFLDYGEEAMKPLVLHDIAEAVGMHEST
ISRVTTQKYMHTPRGIFELKYFFSSHVSTAEGGECSSTAIRAIIKKLVAAENAKKPLSDSKIAGLLEAQGIQVARRTVAK
YRESLGIAPSSERKRLV

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=844907 V2199_RS23465 WP_003094357.1 5062624..5064117(-) (rpoN/rpoN1) [Pseudomonas aeruginosa strain PA26_WM3_03.23_ST27]
ATGAAACCATCGCTAGTCCTCAAGATGGGCCAGCAGCTGACGATGACGCCGCAGCTGCAACAGGCCATACGCCTCCTCCA
GCTATCCACCCTGGACCTCCAGCAGGAAATCCAGGAAGCGCTGGAATCCAATCCCATGCTCGAACGCCAGGAAGACGGCG
ACGATTTCGACAACAGCGACCCTCTCGCCGATGGCGCCGAACAGGCGGCCAGCGCGCCCCAGGAGAGCCCTCTCCAGGAA
AGCGCCACGCCCAGCGTCGAAAGCCTCGACGACGACCAGTGGAGCGAACGCATACCCAGCGAGTTGCCGGTGGATACCGC
CTGGGAAGACATCTACCAGACCAGCGCCAGCAGCCTGCCCAGCAACGACGATGACGAGTGGGACTTCACCGCGCGCACCA
GCAGCGGCGAGAGCCTGCACAGCCACCTGCTCTGGCAGGTCAACCTGGCACCGATGTCGGACACCGATCGGATGATCGCG
GTGACCATCATCGATAGCATCAACAACGATGGCTATCTCGAAGAGTCCCTCGAAGAAATCCTCGCCGCCATCGATCCGGA
ACTGGACGTCGAGCTCGACGAAGTGGAAGTCGTGCTGCGCCGCATCCAGCAACTGGAACCCGCCGGCATCGGCGCCCGCA
ACCTGCGCGAGTGCCTGCTGCTGCAACTGCGCCAGTTGCCGTCCACCACGCCCTGGCTGAACGAAGCGCTGCGCCTGGTA
TCCGACTACCTCGACCTGCTCGGCGGCCGCGACTACAGCCAATTGATGCGGCGCATGAAGCTCAAGGAAGACGAACTGCG
CCAGGTCATCGAGCTGATCCAGTGCCTGCATCCCCGGCCCGGTTCGCAGATCGAATCGAGCGAAGCCGAATACATCGTCC
CCGACGTCATCGTGCGCAAGGACAACGAGCGCTGGCTGGTCGAGCTGAACCAGGAGGCGATGCCACGCCTGCGCGTCAAC
GCCACCTACGCCGGCATGGTCCGCCGCGCCGACAGCAGCGCCGACAATACCTTCATGCGCAACCAGTTGCAGGAAGCCCG
CTGGTTCATCAAGAGCCTGCAGAGCCGCAACGAAACGCTGATGAAGGTCGCCACCCAGATCGTCGAGCACCAGCGCGGTT
TCCTCGACTACGGCGAAGAAGCCATGAAGCCGCTGGTCCTGCACGACATCGCCGAAGCGGTGGGCATGCACGAATCGACC
ATTTCGCGGGTCACCACGCAGAAATACATGCATACGCCGCGCGGCATTTTCGAGCTGAAATATTTCTTTTCAAGTCACGT
CAGTACCGCCGAAGGCGGGGAATGCTCGTCCACCGCGATCCGCGCGATCATCAAGAAACTGGTGGCCGCGGAAAATGCGA
AAAAGCCATTGAGCGACAGCAAGATCGCTGGTTTACTGGAGGCACAGGGCATTCAAGTGGCTCGCCGCACCGTCGCCAAA
TACCGCGAATCCCTGGGCATAGCCCCTTCGAGCGAGCGCAAGCGACTGGTGTGA

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(337-495)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(129-323)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZGR9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

46.654

100

0.477