Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA1   Type   Machinery gene
Locus tag   JMH49_RS06580 Genome accession   NZ_AP024270
Coordinates   1240978..1241448 (+) Length   156 a.a.
NCBI ID   WP_201350754.1    Uniprot ID   -
Organism   Thermus thermophilus strain HB5018     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1235978..1246448
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JMH49_RS06560 (TthHB5018_13010) ilvC 1236470..1237483 (-) 1014 WP_011173279.1 ketol-acid reductoisomerase -
  JMH49_RS06565 (TthHB5018_13020) ilvN 1237480..1237992 (-) 513 WP_096412614.1 acetolactate synthase small subunit -
  JMH49_RS06570 (TthHB5018_13030) ilvB 1237989..1239677 (-) 1689 WP_024119431.1 biosynthetic-type acetolactate synthase large subunit -
  JMH49_RS06575 (TthHB5018_13040) - 1239832..1240812 (-) 981 WP_201350753.1 YpdA family putative bacillithiol disulfide reductase -
  JMH49_RS06580 (TthHB5018_13050) pilA/pilA1 1240978..1241448 (+) 471 WP_201350754.1 GspH/FimT family protein Machinery gene
  JMH49_RS06585 (TthHB5018_13060) - 1241503..1241970 (+) 468 WP_011228523.1 GspH/FimT family protein -
  JMH49_RS06590 (TthHB5018_13070) - 1241996..1242328 (+) 333 WP_224065211.1 prepilin -
  JMH49_RS06595 (TthHB5018_13080) - 1242325..1243035 (+) 711 WP_011228525.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  JMH49_RS06600 (TthHB5018_13090) - 1243049..1244548 (+) 1500 WP_201350755.1 pilus assembly PilX N-terminal domain-containing protein -
  JMH49_RS14245 (TthHB5018_13100) - 1244812..1245168 (+) 357 WP_201350756.1 type II secretion system protein -
  JMH49_RS06610 (TthHB5018_13110) - 1245210..1245575 (+) 366 WP_201350757.1 type II secretion system protein -
  JMH49_RS06615 (TthHB5018_13120) - 1245651..1246304 (+) 654 WP_201350758.1 DUF1640 domain-containing protein -

Sequence

Protein


Download         Length: 156 a.a.        Molecular weight: 16873.68 Da        Isoelectric Point: 11.7108

>NTDB_id=84440 JMH49_RS06580 WP_201350754.1 1240978..1241448(+) (pilA/pilA1) [Thermus thermophilus strain HB5018]
MTRRGLTLLELLLVLGILGVLLGLALPLLSPNRLALDQAARSLAAQVTRARLEAIRRNAFVGLQVFTEGAGGYLLFVDQN
ANRRYDPGEEFGATRFGQGNWARVRLDPEKSALGNMPLLFDPRGIPAKPITATLVLTSGGATRKVVISQQGRARLE

Nucleotide


Download         Length: 471 bp        

>NTDB_id=84440 JMH49_RS06580 WP_201350754.1 1240978..1241448(+) (pilA/pilA1) [Thermus thermophilus strain HB5018]
ATGACTCGGCGGGGCCTCACCCTTTTGGAGCTCCTGCTGGTCTTGGGCATCCTGGGGGTCCTCCTAGGGCTGGCCCTTCC
ACTTCTTTCCCCCAACCGCCTCGCCCTGGACCAGGCCGCCCGCTCCCTCGCCGCCCAGGTGACCCGGGCAAGGCTCGAGG
CCATCCGCCGGAACGCCTTCGTGGGCCTTCAGGTGTTCACCGAAGGAGCGGGGGGTTACCTGCTCTTCGTGGACCAAAAC
GCCAACCGCCGCTACGACCCCGGGGAGGAGTTCGGCGCGACCCGCTTCGGCCAAGGGAACTGGGCCCGGGTGCGCCTGGA
CCCGGAGAAGAGCGCCCTTGGCAACATGCCCCTTCTCTTTGACCCCCGGGGCATCCCGGCCAAGCCCATCACCGCCACGC
TCGTCCTCACCTCAGGGGGCGCCACCCGCAAGGTGGTCATCTCCCAGCAGGGCCGGGCCCGCCTGGAGTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(3-26)

Type II transport protein GspH (GspH)

(39-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA1 Thermus thermophilus HB27

98.718

100

0.987

Multiple sequence alignment