Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   DBT50_RS00365 Genome accession   NZ_CP127382
Coordinates   73832..75220 (+) Length   462 a.a.
NCBI ID   WP_111852712.1    Uniprot ID   -
Organism   Aerococcus tenax strain UMB3669     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 68832..80220
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DBT50_RS00350 (DBT50_006365) - 69895..72822 (+) 2928 WP_146742909.1 insulinase family protein -
  DBT50_RS00355 (DBT50_006370) - 72949..73134 (-) 186 WP_111852710.1 hypothetical protein -
  DBT50_RS00360 (DBT50_006375) - 73303..73812 (+) 510 WP_111852711.1 dUTP diphosphatase -
  DBT50_RS00365 (DBT50_006380) radA 73832..75220 (+) 1389 WP_111852712.1 DNA repair protein RadA Machinery gene
  DBT50_RS00370 (DBT50_006385) - 75307..76479 (+) 1173 WP_111821675.1 PIN/TRAM domain-containing protein -
  DBT50_RS00375 (DBT50_006390) - 76591..77406 (-) 816 WP_111852713.1 HAD family hydrolase -
  DBT50_RS00380 (DBT50_006395) - 77573..78274 (+) 702 WP_111852714.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 462 a.a.        Molecular weight: 50415.64 Da        Isoelectric Point: 6.4078

>NTDB_id=843871 DBT50_RS00365 WP_111852712.1 73832..75220(+) (radA) [Aerococcus tenax strain UMB3669]
MAKKKRKTVYTCQACGYESPQWYGKCPQCGAWNTLEEEVIAGKPIRQAGTEVNQKKAKPERLKEVSQAEEKRVKTGLGEF
NRVLGGGVVPGSLVLIGGDPGIGKSTLLLQVAKQLSDNGGRVLYVSGEESLHQIKMRADRLGYQDADFFVYAETDLLAIE
AAISDTQADFVVIDSIQTMVHPNNESLAGSVSQVREATGELMHIAKSSGIAIFVVGHVTKEGNIAGPRILEHMVDTVLYL
EGEKHNTFRILRAVKNRFGSTNEIGVFDMKSQGLEEVTNPSQLFLEERLMGANGSAVVASMEGTRPILTEIQALLSATAF
GNARRTASGLDYSRVTLIMAVLEKRAHLMLQNQDAYLKSTGGVKLDEPAIDLAIAIAVASSYWEKETQASDCFVGEIGLT
GEIRRISRINERIQEAEKLGFKRIFIPYGNLQGLATDSQDIEIIGAKTLTQVLKEVFPPQGR

Nucleotide


Download         Length: 1389 bp        

>NTDB_id=843871 DBT50_RS00365 WP_111852712.1 73832..75220(+) (radA) [Aerococcus tenax strain UMB3669]
ATGGCTAAAAAGAAGCGAAAAACTGTCTACACCTGCCAGGCCTGTGGCTATGAATCGCCTCAGTGGTATGGGAAATGCCC
CCAATGTGGAGCTTGGAATACCCTGGAAGAGGAAGTGATTGCGGGTAAGCCCATCCGCCAAGCTGGGACAGAAGTCAATC
AGAAAAAAGCCAAGCCTGAACGTCTCAAGGAAGTCAGCCAGGCGGAAGAAAAACGGGTAAAGACTGGCTTAGGCGAGTTC
AACCGGGTCCTCGGTGGCGGGGTAGTCCCTGGGTCTTTGGTCCTGATTGGTGGGGATCCTGGTATTGGGAAGTCGACCCT
GCTCCTCCAGGTAGCTAAGCAATTAAGTGATAATGGTGGCCGAGTTCTCTATGTTTCTGGGGAAGAAAGTTTGCATCAAA
TCAAGATGCGGGCCGACCGCTTGGGCTACCAGGATGCGGACTTCTTTGTTTATGCCGAAACCGACCTCTTAGCCATTGAA
GCGGCTATTTCTGATACCCAGGCGGATTTTGTGGTGATTGACTCCATCCAAACCATGGTCCATCCCAATAATGAATCTTT
AGCCGGTAGTGTCAGCCAGGTCAGAGAAGCGACCGGAGAACTCATGCATATCGCCAAAAGTTCTGGGATCGCCATCTTTG
TCGTCGGCCATGTGACCAAGGAAGGTAATATTGCCGGACCGCGGATTCTCGAACATATGGTGGACACGGTCTTATACTTG
GAAGGGGAGAAACACAACACCTTCCGGATCCTCAGAGCGGTCAAAAACCGTTTTGGGTCGACCAATGAAATCGGCGTTTT
TGACATGAAGAGCCAGGGACTGGAAGAGGTCACCAATCCCAGCCAGCTCTTTTTAGAAGAACGGCTCATGGGAGCCAATG
GGTCGGCGGTGGTCGCTTCCATGGAAGGAACCCGTCCGATCCTAACCGAAATCCAAGCCCTCTTGTCGGCCACGGCCTTT
GGTAATGCCCGTCGTACGGCCAGTGGTTTGGACTATTCCCGGGTGACTCTCATTATGGCGGTCTTAGAGAAGCGGGCCCA
TCTCATGTTGCAAAACCAAGACGCCTATCTCAAGTCAACCGGTGGGGTTAAACTGGATGAACCAGCCATTGACCTAGCTA
TTGCTATAGCTGTGGCTTCCAGTTACTGGGAGAAGGAGACCCAGGCCAGCGATTGCTTTGTGGGTGAGATTGGCCTAACC
GGTGAAATTCGCCGGATTTCCCGGATTAATGAACGAATCCAAGAAGCGGAAAAATTAGGATTTAAACGGATTTTTATCCC
CTATGGGAATCTCCAGGGCCTGGCGACTGATAGTCAAGATATTGAAATTATTGGCGCCAAGACCCTGACTCAAGTTCTCA
AAGAAGTCTTTCCCCCTCAGGGGCGATAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(68-222)

Subunit ChlI of Mg-chelatase (ChlI)

(367-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(10-37)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

62.939

98.701

0.621
  radA Streptococcus pneumoniae Rx1

63.135

98.052

0.619
  radA Streptococcus pneumoniae D39

63.135

98.052

0.619
  radA Streptococcus mitis SK321

63.135

98.052

0.619
  radA Streptococcus pneumoniae TIGR4

63.135

98.052

0.619
  radA Streptococcus pneumoniae R6

63.135

98.052

0.619
  radA Streptococcus mitis NCTC 12261

62.914

98.052

0.617