Detailed information NTG0843659

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: V2I96_RS00900
• Genome accession: NZ_CP143627
• Coordinates: 178223..179206 (+)
• Length: 327 a.a.
• NCBI ID: WP_001196486.1
• Uniprot ID: A7ZT85
• Organism: Escherichia coli strain FJ_Ec_21
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 173223..184206

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V2I96_RS00880 (V2I96_00880)	dppA	174366..175973 (+)	1608	WP_001222883.1	dipeptide ABC transporter substrate-binding protein DppA	-
V2I96_RS00890 (V2I96_00890)	dppB	176281..177300 (+)	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-
V2I96_RS00895 (V2I96_00895)	dppC	177310..178212 (+)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
V2I96_RS00900 (V2I96_00900)	amiE	178223..179206 (+)	984	WP_001196486.1	dipeptide ABC transporter ATP-binding protein	Regulator
V2I96_RS00905 (V2I96_00905)	dppF	179203..180207 (+)	1005	WP_000107012.1	dipeptide ABC transporter ATP-binding subunit DppF	-
V2I96_RS00910 (V2I96_00910)	yhjV	180237..181508 (-)	1272	WP_001295225.1	aromatic amino acid transport family protein	-
V2I96_RS00915 (V2I96_00915)	ldrD	181984..182091 (+)	108	WP_000141634.1	type I toxin-antitoxin system toxic polypeptide LdrD	-
V2I96_RS00920 (V2I96_00920)	bcsG	182178..183857 (-)	1680	WP_000191622.1	cellulose biosynthesis protein BcsG	-
V2I96_RS00925 (V2I96_00925)	bcsF	183854..184045 (-)	192	WP_000988308.1	cellulose biosynthesis protein BcsF	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35844.36 Da
• Isoelectric Point: 6.5814

>NTDB_id=843659 V2I96_RS00900 WP_001196486.1 178223..179206(+) (amiE) [Escherichia coli strain FJ_Ec_21]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=843659 V2I96_RS00900 WP_001196486.1 178223..179206(+) (amiE) [Escherichia coli strain FJ_Ec_21]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCGCCGTTCCGCGCCGTAGACCGCATCAG
CTACAGCGTAAAACAGGGCGAAGTGGTCGGGATTGTGGGTGAGTCCGGCTCCGGTAAGTCGGTCAGTTCACTGGCGATTA
TGGGGCTGATTGATTATCCGGGCCGCGTAATGGCAGAAAAACTGGAGTTTAACGGCCAGGATTTGCAGCGTATCTCAGAA
AAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACAC
CGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCATCAGGGCGGCAACAAAAGTACCCGCCGTCAGCGAGCGATTGACC
TGCTGAATCAGGTCGGTATTCCCGATCCGGCATCGCGTCTGGATGTTTACCCGCATCAGCTTTCCGGCGGCATGAGCCAG
CGCGTGATGATCGCCATGGCGATTGCCTGTCGGCCAAAACTGCTGATTGCCGATGAACCGACCACCGCGCTGGACGTGAC
CATTCAGGCGCAAATCATCGAACTACTGCTGGAGCTACAGCAGAAAGAGAACATGGCGCTGGTGTTAATTACCCATGACC
TGGCGCTGGTGGCGGAAGCGGCACATAAAATCATCGTGATGTATGCAGGCCAGGTGGTGGAAACCGGTGATGCGCACGCC
ATCTTCCATGCGCCGCGTCACCCGTATACTCAGGCATTGCTGCGTGCGCTGCCAGAATTTGCTCAGGACAAAGAACGTCT
GGCGTCGTTGCCAGGTGTCGTTCCCGGCAAGTACGACCGCCCGAACGGCTGCCTGCTTAACCCGCGCTGCCCCTATGCCA
CTGACAGATGTCGCGCTGAAGAACCGGCGCTGAATATGCTCGCTGACGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

• ABC transporter (ABC_tran) (24-181)

3D structure

• AlphaFold DB: A7ZT85

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404