Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   QRA07_RS16960 Genome accession   NZ_CP127321
Coordinates   3561726..3562526 (-) Length   266 a.a.
NCBI ID   WP_002114699.1    Uniprot ID   -
Organism   Acinetobacter pittii strain PC870     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3556726..3567526
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QRA07_RS16940 (QRA07_16985) - 3557791..3558489 (-) 699 WP_233734309.1 tetratricopeptide repeat protein -
  QRA07_RS16945 (QRA07_16990) hisS 3558518..3559810 (-) 1293 WP_170247644.1 histidine--tRNA ligase -
  QRA07_RS16950 (QRA07_16995) ispG 3559807..3560922 (-) 1116 WP_002115078.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  QRA07_RS16955 (QRA07_17000) - 3560944..3561735 (-) 792 WP_202745055.1 helix-turn-helix domain-containing protein -
  QRA07_RS16960 (QRA07_17005) pilF 3561726..3562526 (-) 801 WP_002114699.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  QRA07_RS16965 (QRA07_17010) rlmN 3562541..3563776 (-) 1236 WP_002115097.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  QRA07_RS16970 (QRA07_17015) ndk 3563900..3564331 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  QRA07_RS16975 (QRA07_17020) iscX 3564445..3564645 (-) 201 WP_004698764.1 Fe-S cluster assembly protein IscX -
  QRA07_RS16980 (QRA07_17025) - 3564745..3565296 (-) 552 WP_005066176.1 phosphatase PAP2 family protein -
  QRA07_RS16985 (QRA07_17030) - 3565321..3566610 (-) 1290 WP_104075006.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30114.38 Da        Isoelectric Point: 9.0417

>NTDB_id=843419 QRA07_RS16960 WP_002114699.1 3561726..3562526(-) (pilF) [Acinetobacter pittii strain PC870]
MSIPKLKTVLCMGIAVAFLVSGCQTTHTQKKDPEKAVKVRTQLAAEHIRSGDLDSAKRALDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQAHNNYGTYLYQMERYNDAVEQFRIAGATLGYDQRYQALENLGRIYLKLGDV
ANAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGVRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=843419 QRA07_RS16960 WP_002114699.1 3561726..3562526(-) (pilF) [Acinetobacter pittii strain PC870]
TTGAGTATTCCAAAGTTAAAAACGGTCTTATGTATGGGGATTGCAGTTGCATTTTTGGTAAGTGGTTGCCAGACAACGCA
TACGCAAAAAAAAGACCCAGAGAAAGCGGTAAAGGTGCGTACACAACTTGCTGCTGAACATATCCGTTCGGGTGACTTGG
ACTCAGCAAAGCGAGCTCTTGATCAAGCTTTAAGTGTAGATAGCCGCGATGCTACTGCAAATATGATGATGGGTATTTTG
CTACAGCAAGAAGGTAGCAAACCCAATTTAGAAAAAGCTGAACATTATTTTAAACGAGCAATTTCATCTGAACCGGATAA
TGCTCAAGCACATAATAATTATGGTACTTATTTATATCAAATGGAGCGTTATAATGATGCGGTTGAGCAATTTCGTATTG
CTGGCGCAACATTAGGTTATGACCAACGCTATCAGGCATTAGAAAATCTGGGGCGTATTTATTTAAAATTGGGTGATGTT
GCGAATGCTGAAAAAACATTCAAACAAGCACTCCTTGCTAACCGCGACTCTTATATTTCAATGTTAGAGTTAGCGGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAGCAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCAAGAGCACTTTGGATTGGTGTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTGTTAGTAAACCAG
TTACGTGCTTTGTTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_8)

(39-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

93.985

100

0.94
  pilF Acinetobacter baylyi ADP1

76.154

97.744

0.744