Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   VYI50_RS19420 Genome accession   NZ_CP142956
Coordinates   4052496..4052936 (+) Length   146 a.a.
NCBI ID   WP_000360914.1    Uniprot ID   A0A0H2YVZ3
Organism   Escherichia coli strain EC200461     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4047496..4057936
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VYI50_RS19400 (VYI50_19400) aroP 4048494..4049867 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  VYI50_RS19405 (VYI50_19405) ampE 4049910..4050764 (-) 855 WP_000172019.1 beta-lactamase regulator AmpE -
  VYI50_RS19410 (VYI50_19410) ampD 4050761..4051312 (-) 552 WP_022645179.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  VYI50_RS19415 (VYI50_19415) nadC 4051400..4052293 (+) 894 WP_001135167.1 carboxylating nicotinate-nucleotide diphosphorylase -
  VYI50_RS19420 (VYI50_19420) pilA 4052496..4052936 (+) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  VYI50_RS19425 (VYI50_19425) pilB 4052946..4054331 (+) 1386 WP_022645178.1 type II secretion system protein GspE Machinery gene
  VYI50_RS19430 (VYI50_19430) hofC 4054321..4055523 (+) 1203 WP_022645177.1 protein transport protein HofC -
  VYI50_RS19435 (VYI50_19435) guaC 4055558..4056601 (-) 1044 WP_001217337.1 GMP reductase -
  VYI50_RS19440 (VYI50_19440) - 4056757..4056801 (-) 45 WP_120795372.1 protein YacM -
  VYI50_RS19445 (VYI50_19445) coaE 4056826..4057446 (+) 621 WP_022645176.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=843174 VYI50_RS19420 WP_000360914.1 4052496..4052936(+) (pilA) [Escherichia coli strain EC200461]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=843174 VYI50_RS19420 WP_000360914.1 4052496..4052936(+) (pilA) [Escherichia coli strain EC200461]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCTTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363