Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   VYI58_RS02185 Genome accession   NZ_CP142953
Coordinates   453873..454550 (-) Length   225 a.a.
NCBI ID   WP_000178170.1    Uniprot ID   -
Organism   Escherichia coli strain EC190759     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 448873..459550
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VYI58_RS02170 (VYI58_02170) chiA 450231..452933 (+) 2703 WP_000773180.1 bifunctional chitinase/lysozyme -
  VYI58_RS02175 (VYI58_02175) bfd 453102..453296 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  VYI58_RS02180 (VYI58_02180) bfr 453368..453844 (+) 477 WP_000675504.1 bacterioferritin -
  VYI58_RS02185 (VYI58_02185) pilD 453873..454550 (-) 678 WP_000178170.1 prepilin peptidase GspO Machinery gene
  VYI58_RS02190 (VYI58_02190) gspM 454550..455011 (-) 462 WP_001296465.1 type II secretion system protein GspM -
  VYI58_RS02195 (VYI58_02195) gspL 455008..456171 (-) 1164 WP_001296464.1 type II secretion system protein GspL -
  VYI58_RS02200 (VYI58_02200) gspK 456186..457169 (-) 984 WP_001058509.1 type II secretion system minor pseudopilin GspK -
  VYI58_RS02205 (VYI58_02205) gspJ 457162..457749 (-) 588 WP_000610632.1 type II secretion system minor pseudopilin GspJ -
  VYI58_RS02210 (VYI58_02210) gspI 457742..458119 (-) 378 WP_001041749.1 type II secretion system minor pseudopilin GspI -
  VYI58_RS02215 (VYI58_02215) gspH 458116..458625 (-) 510 WP_001076039.1 type II secretion system minor pseudopilin GspH -
  VYI58_RS02220 (VYI58_02220) gspG 458633..459070 (-) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24842.69 Da        Isoelectric Point: 7.6364

>NTDB_id=843038 VYI58_RS02185 WP_000178170.1 453873..454550(-) (pilD) [Escherichia coli strain EC190759]
MTMLLPLFILVGFIAGYFVNAMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=843038 VYI58_RS02185 WP_000178170.1 453873..454550(-) (pilD) [Escherichia coli strain EC190759]
ATGACAATGTTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.95

100

0.382