Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   VYI52_RS20210 Genome accession   NZ_CP142949
Coordinates   4138134..4138574 (+) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain EC190329     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4133134..4143574
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VYI52_RS20190 (VYI52_20190) aroP 4134132..4135505 (+) 1374 WP_001347572.1 aromatic amino acid transporter AroP -
  VYI52_RS20195 (VYI52_20195) ampE 4135548..4136402 (-) 855 WP_000172000.1 beta-lactamase regulator AmpE -
  VYI52_RS20200 (VYI52_20200) ampD 4136399..4136950 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  VYI52_RS20205 (VYI52_20205) nadC 4137038..4137931 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  VYI52_RS20210 (VYI52_20210) pilA 4138134..4138574 (+) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  VYI52_RS20215 (VYI52_20215) pilB 4138584..4139969 (+) 1386 WP_001025174.1 type II secretion system protein GspE Machinery gene
  VYI52_RS20220 (VYI52_20220) hofC 4139959..4141161 (+) 1203 WP_000157236.1 protein transport protein HofC -
  VYI52_RS20225 (VYI52_20225) guaC 4141196..4142239 (-) 1044 WP_001217338.1 GMP reductase -
  VYI52_RS20230 (VYI52_20230) - 4142395..4142439 (-) 45 WP_120795372.1 protein YacM -
  VYI52_RS20235 (VYI52_20235) coaE 4142464..4143084 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=843004 VYI52_RS20210 WP_000360895.1 4138134..4138574(+) (pilA) [Escherichia coli strain EC190329]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=843004 VYI52_RS20210 WP_000360895.1 4138134..4138574(+) (pilA) [Escherichia coli strain EC190329]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363