Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   VYI62_RS20945 Genome accession   NZ_CP142925
Coordinates   4277894..4278334 (-) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain B20105     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4272894..4283334
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VYI62_RS20920 (VYI62_20915) coaE 4273384..4274004 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  VYI62_RS20925 (VYI62_20920) - 4274029..4274073 (+) 45 WP_120795372.1 protein YacM -
  VYI62_RS20930 (VYI62_20925) guaC 4274229..4275272 (+) 1044 WP_001217338.1 GMP reductase -
  VYI62_RS20935 (VYI62_20930) hofC 4275307..4276509 (-) 1203 WP_000157236.1 protein transport protein HofC -
  VYI62_RS20940 (VYI62_20935) pilB 4276499..4277884 (-) 1386 WP_001025174.1 type II secretion system protein GspE Machinery gene
  VYI62_RS20945 (VYI62_20940) pilA 4277894..4278334 (-) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  VYI62_RS20950 (VYI62_20945) nadC 4278537..4279430 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  VYI62_RS20955 (VYI62_20950) ampD 4279518..4280069 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  VYI62_RS20960 (VYI62_20955) ampE 4280066..4280920 (+) 855 WP_000172000.1 beta-lactamase regulator AmpE -
  VYI62_RS20965 (VYI62_20960) aroP 4280963..4282336 (-) 1374 WP_001347572.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=842677 VYI62_RS20945 WP_000360895.1 4277894..4278334(-) (pilA) [Escherichia coli strain B20105]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=842677 VYI62_RS20945 WP_000360895.1 4277894..4278334(-) (pilA) [Escherichia coli strain B20105]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363