Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   QN079_RS00110 Genome accession   NZ_CP127160
Coordinates   22741..24111 (+) Length   456 a.a.
NCBI ID   WP_289230176.1    Uniprot ID   -
Organism   Enterococcus sp. FZMF     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 17741..29111
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QN079_RS00080 (QN079_00080) - 18208..19026 (+) 819 WP_005232914.1 ZIP family metal transporter -
  QN079_RS00085 (QN079_00085) - 19082..19285 (-) 204 WP_010748476.1 hypothetical protein -
  QN079_RS00090 (QN079_00090) - 19436..19837 (+) 402 WP_289230174.1 DUF4809 family protein -
  QN079_RS00095 (QN079_00095) - 19941..21185 (-) 1245 WP_289230175.1 glutamate-5-semialdehyde dehydrogenase -
  QN079_RS00100 (QN079_00100) proB 21182..21997 (-) 816 WP_005226390.1 glutamate 5-kinase -
  QN079_RS00105 (QN079_00105) - 22187..22696 (+) 510 WP_010748479.1 hypothetical protein -
  QN079_RS00110 (QN079_00110) radA 22741..24111 (+) 1371 WP_289230176.1 DNA repair protein RadA Machinery gene
  QN079_RS00115 (QN079_00115) - 24267..25406 (+) 1140 WP_005226396.1 PIN/TRAM domain-containing protein -
  QN079_RS00120 (QN079_00120) gltX 25576..27033 (+) 1458 WP_289230177.1 glutamate--tRNA ligase -
  QN079_RS00125 (QN079_00125) epsC 27294..27830 (+) 537 WP_026008130.1 serine O-acetyltransferase EpsC -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49646.36 Da        Isoelectric Point: 8.3034

>NTDB_id=842130 QN079_RS00110 WP_289230176.1 22741..24111(+) (radA) [Enterococcus sp. FZMF]
MAKKAKSQFVCQNCGYSSPKYLGKCPNCGSWNSMVEEVIQETADRRVRTTLTGQKMQPTKIADVVPKKEPRVQTKLAELN
RVLGGGVVPGSLILIGGDPGIGKSTLLLQVSQQLAAIGGTVLYVSGEESAEQIKMRAQRLGAIDTEFYLYVETDMSEISK
AIENIRPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVTKEGSIAGPRMLEHMVDTVLYFE
GEKHHSFRILRAVKNRFGSTNEIGIFEMEEGGLKEVANPSQVFLEERLEGATGSAIVVAMEGSRPILVEIQALVTPTMFG
NAKRTTTGLDFNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTSPTECFIGEIGLTG
EIRRVNAIEQRVKEVQKLGFTKVYLPKNNLTGWQPPKGIEVEGVSTLAETLRKVFR

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=842130 QN079_RS00110 WP_289230176.1 22741..24111(+) (radA) [Enterococcus sp. FZMF]
ATGGCAAAAAAAGCAAAAAGTCAATTTGTCTGTCAAAATTGTGGGTATAGTTCCCCTAAATATTTAGGGAAATGCCCCAA
TTGCGGCAGCTGGAATTCAATGGTGGAAGAAGTGATCCAAGAAACCGCCGATCGTCGAGTACGTACGACATTGACGGGGC
AAAAGATGCAGCCGACGAAAATCGCCGATGTCGTACCGAAAAAAGAACCGCGGGTCCAAACGAAACTCGCTGAGTTGAAT
CGCGTCTTAGGTGGCGGCGTGGTCCCAGGCTCCTTGATTTTGATCGGTGGGGATCCAGGGATCGGAAAATCGACGCTGCT
CTTGCAAGTTTCCCAGCAATTAGCAGCGATCGGTGGGACGGTCTTGTATGTGTCAGGAGAAGAAAGTGCCGAGCAGATCA
AAATGCGGGCGCAGCGCTTAGGCGCCATCGACACGGAATTTTATCTCTATGTGGAAACGGACATGTCAGAGATCAGTAAA
GCGATCGAAAACATCCGCCCAGATTATGTGATCATCGATTCGATCCAAACCATGACCCAACCGGATATCACCAGCGTGGC
AGGCAGTGTCAGCCAAGTGCGAGAAACGACCGCAGAATTGCTGAAAATCGCCAAAACCAATGGCATCGCCATCTTTATCG
TTGGCCACGTGACGAAGGAAGGCTCGATCGCAGGACCGCGGATGCTGGAGCATATGGTGGATACCGTCTTGTATTTCGAA
GGCGAAAAGCACCACAGTTTTCGAATTTTGCGGGCCGTCAAAAACCGCTTTGGGTCCACCAATGAGATCGGGATTTTTGA
AATGGAAGAAGGCGGGTTGAAAGAAGTCGCCAATCCGTCACAAGTCTTTTTGGAAGAACGCTTAGAAGGCGCAACCGGAT
CGGCGATCGTAGTAGCAATGGAAGGCAGTCGGCCAATCTTGGTGGAGATCCAAGCACTGGTCACGCCAACGATGTTCGGC
AATGCCAAACGCACGACGACGGGGCTAGACTTCAACCGCGTCTCGCTGATCATGGCGGTTTTAGAAAAACGGGCAGGCTT
GCTGCTGCAAAATCAAGATGCCTACTTAAAAGCAGCCGGCGGGGTCAAATTAAATGAACCTGCGATCGATCTAGCGATCG
CTGTTAGTATCGCATCTAGCTACAAAGAAAAAGGCACCAGCCCGACGGAGTGTTTTATCGGTGAAATTGGCTTGACGGGT
GAGATTCGCCGAGTCAACGCGATCGAGCAACGGGTCAAAGAGGTGCAAAAACTAGGTTTTACGAAAGTTTATTTGCCGAA
AAATAATCTGACTGGCTGGCAGCCGCCCAAAGGCATCGAAGTCGAAGGCGTGTCTACCTTAGCCGAAACCTTGCGGAAAG
TCTTCCGTTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)

AAA domain (AAA_25)

(71-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

74.725

99.781

0.746
  radA Streptococcus mitis SK321

74.505

99.781

0.743
  radA Streptococcus pneumoniae Rx1

74.505

99.781

0.743
  radA Streptococcus pneumoniae D39

74.505

99.781

0.743
  radA Streptococcus pneumoniae R6

74.505

99.781

0.743
  radA Streptococcus pneumoniae TIGR4

74.505

99.781

0.743
  radA Bacillus subtilis subsp. subtilis str. 168

67.184

98.904

0.664