Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   VVK16_RS07340 Genome accession   NZ_CP142708
Coordinates   422136..422813 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain T28R     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 417136..427813
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VVK16_RS07325 (VVK16_07315) chiA 418503..421196 (+) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -
  VVK16_RS07330 (VVK16_07320) bfd 421365..421559 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  VVK16_RS07335 (VVK16_07325) bfr 421631..422107 (+) 477 WP_000675504.1 bacterioferritin -
  VVK16_RS07340 (VVK16_07330) pilD 422136..422813 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  VVK16_RS07345 (VVK16_07335) gspM 422813..423274 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  VVK16_RS07350 (VVK16_07340) gspL 423271..424434 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  VVK16_RS07355 (VVK16_07345) gspK 424449..425432 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  VVK16_RS07360 (VVK16_07350) gspJ 425425..426012 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  VVK16_RS07365 (VVK16_07355) gspI 426005..426382 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  VVK16_RS07370 (VVK16_07360) gspH 426379..426888 (-) 510 WP_112912617.1 type II secretion system minor pseudopilin GspH -
  VVK16_RS07375 (VVK16_07365) gspG 426896..427333 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=841708 VVK16_RS07340 WP_000178161.1 422136..422813(-) (pilD) [Escherichia coli strain T28R]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=841708 VVK16_RS07340 WP_000178161.1 422136..422813(-) (pilD) [Escherichia coli strain T28R]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378