Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   VR550_RS01920 Genome accession   NZ_CP142677
Coordinates   411922..412599 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain FG92-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 406922..417599
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VR550_RS01905 (VR550_01895) chiA 408289..410982 (+) 2694 WP_104774102.1 bifunctional chitinase/lysozyme -
  VR550_RS01910 (VR550_01900) bfd 411151..411345 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  VR550_RS01915 (VR550_01905) bfr 411417..411893 (+) 477 WP_000675504.1 bacterioferritin -
  VR550_RS01920 (VR550_01910) pilD 411922..412599 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  VR550_RS01925 (VR550_01915) gspM 412599..413060 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  VR550_RS01930 (VR550_01920) gspL 413057..414220 (-) 1164 WP_072778696.1 type II secretion system protein GspL -
  VR550_RS01935 (VR550_01925) gspK 414235..415218 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  VR550_RS01940 (VR550_01930) gspJ 415211..415799 (-) 589 Protein_384 type II secretion system minor pseudopilin GspJ -
  VR550_RS01945 (VR550_01935) gspI 415792..416169 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  VR550_RS01950 (VR550_01940) gspH 416166..416675 (-) 510 WP_001076046.1 type II secretion system minor pseudopilin GspH -
  VR550_RS01955 (VR550_01945) gspG 416683..417120 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=841547 VR550_RS01920 WP_000178161.1 411922..412599(-) (pilD) [Escherichia coli strain FG92-1]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=841547 VR550_RS01920 WP_000178161.1 411922..412599(-) (pilD) [Escherichia coli strain FG92-1]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378