Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   QQW97_RS13405 Genome accession   NZ_CP127089
Coordinates   2495134..2495544 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis strain KK026     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2490134..2500544
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QQW97_RS13375 (QQW97_13370) - 2490221..2490361 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  QQW97_RS13380 (QQW97_13375) yqeF 2490667..2491398 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  QQW97_RS13385 (QQW97_13380) cwlH 2491650..2492402 (-) 753 WP_003229963.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  QQW97_RS13390 (QQW97_13385) yqeD 2492589..2493215 (+) 627 WP_003229962.1 TVP38/TMEM64 family protein -
  QQW97_RS13395 (QQW97_13390) gnd 2493234..2494127 (-) 894 WP_003229961.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  QQW97_RS13400 (QQW97_13395) yqeB 2494379..2495101 (+) 723 WP_010886572.1 hypothetical protein -
  QQW97_RS13405 (QQW97_13400) nucA/comI 2495134..2495544 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  QQW97_RS13410 (QQW97_13405) - 2495740..2496090 (+) 351 Protein_2593 sigma-70 family RNA polymerase sigma factor -
  QQW97_RS13415 (QQW97_13410) spoIVCA 2496118..2497578 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  QQW97_RS13420 (QQW97_13415) - 2497536..2497714 (-) 179 Protein_2595 hypothetical protein -
  QQW97_RS13425 (QQW97_13420) arsC 2498069..2498488 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  QQW97_RS13430 (QQW97_13425) - 2498500..2498628 (-) 129 Protein_2597 arsenical-resistance protein -
  QQW97_RS13435 (QQW97_13430) - 2498626..2499012 (+) 387 Protein_2598 ImmA/IrrE family metallo-endopeptidase -
  QQW97_RS13440 (QQW97_13435) - 2499012..2499410 (+) 399 Protein_2599 sigma-70 family RNA polymerase sigma factor -
  QQW97_RS13445 (QQW97_13440) - 2499410..2499526 (+) 117 WP_003229898.1 hypothetical protein -
  QQW97_RS13450 (QQW97_13445) - 2499492..2499692 (-) 201 Protein_2601 recombinase family protein -
  QQW97_RS13455 (QQW97_13450) - 2499635..2499799 (-) 165 WP_010886576.1 hypothetical protein -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=841546 QQW97_RS13405 WP_009967785.1 2495134..2495544(-) (nucA/comI) [Bacillus subtilis strain KK026]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=841546 QQW97_RS13405 WP_009967785.1 2495134..2495544(-) (nucA/comI) [Bacillus subtilis strain KK026]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529