Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   QMG85_RS25775 Genome accession   NZ_AP027162
Coordinates   5028471..5029139 (+) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli strain EH031     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5023471..5034139
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMG85_RS25745 (BEL031_4945) yicC 5023490..5024353 (-) 864 WP_000621336.1 YicC/YloC family endoribonuclease -
  QMG85_RS25750 (BEL031_4946) rph 5024480..5025196 (+) 717 WP_001247089.1 ribonuclease PH -
  QMG85_RS25755 (BEL031_4947) pyrE 5025262..5025903 (+) 642 WP_000806161.1 orotate phosphoribosyltransferase -
  QMG85_RS25760 (BEL031_4948) slmA 5025940..5026536 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  QMG85_RS25765 (BEL031_4949) dut 5026643..5027101 (-) 459 WP_000976070.1 dUTP diphosphatase -
  QMG85_RS25770 (BEL031_4950) coaBC 5027079..5028299 (-) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  QMG85_RS25775 (BEL031_4951) radC 5028471..5029139 (+) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  QMG85_RS25780 (BEL031_4952) rpmB 5029356..5029592 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  QMG85_RS25785 (BEL031_4953) rpmG 5029613..5029780 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  QMG85_RS25790 (BEL031_4954) mutM 5029878..5030687 (+) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  QMG85_RS25795 (BEL031_4955) coaD 5030726..5031205 (-) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  QMG85_RS25800 (BEL031_4956) waaA 5031213..5032490 (-) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  QMG85_RS25805 (BEL031_4957) rfaQ 5032903..5033961 (+) 1059 WP_000360406.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=84154 QMG85_RS25775 WP_001297375.1 5028471..5029139(+) (radC) [Escherichia coli strain EH031]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=84154 QMG85_RS25775 WP_001297375.1 5028471..5029139(+) (radC) [Escherichia coli strain EH031]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGTTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGCGTTCATGGAATTGGCGTAGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCACAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496

Multiple sequence alignment