Detailed information NTG0841371

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: QQO08_RS08485
• Genome accession: NZ_CP127024
• Coordinates: 1716565..1717062 (-)
• Length: 165 a.a.
• NCBI ID: WP_001803317.1
• Uniprot ID: A0A2I7Y542
• Organism: Staphylococcus aureus strain CC239-MRSA-III(var.) isolate Trinidad&Tobago_2020-048_8421A
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1711565..1722062

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QQO08_RS08460 (QQO08_08460)	gcvPB	1711727..1713199 (-)	1473	WP_000202189.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPB	-
QQO08_RS08465 (QQO08_08465)	gcvPA	1713192..1714538 (-)	1347	WP_000019687.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPA	-
QQO08_RS08470 (QQO08_08470)	gcvT	1714558..1715649 (-)	1092	WP_000093351.1	glycine cleavage system aminomethyltransferase GcvT	-
QQO08_RS08475 (QQO08_08475)	-	1715808..1716332 (-)	525	WP_001015117.1	shikimate kinase	-
QQO08_RS08480 (QQO08_08480)	-	1716322..1716468 (-)	147	WP_001789879.1	hypothetical protein	-
QQO08_RS08485 (QQO08_08485)	comGF	1716565..1717062 (-)	498	WP_001803317.1	competence type IV pilus minor pilin ComGF	Machinery gene
QQO08_RS08490 (QQO08_08490)	comGE	1716980..1717279 (-)	300	WP_000844413.1	hypothetical protein	Machinery gene
QQO08_RS08495 (QQO08_08495)	comGD	1717266..1717712 (-)	447	WP_001790850.1	competence type IV pilus minor pilin ComGD	Machinery gene
QQO08_RS08500 (QQO08_08500)	comGC	1717690..1718001 (-)	312	WP_000472256.1	competence type IV pilus major pilin ComGC	Machinery gene
QQO08_RS08505 (QQO08_08505)	comGB	1718015..1719085 (-)	1071	WP_000775706.1	competence type IV pilus assembly protein ComGB	Machinery gene
QQO08_RS08510 (QQO08_08510)	comGA	1719057..1720031 (-)	975	WP_000697228.1	competence type IV pilus ATPase ComGA	Machinery gene
QQO08_RS08515 (QQO08_08515)	-	1720083..1720706 (-)	624	WP_001223008.1	MBL fold metallo-hydrolase	-
QQO08_RS08520 (QQO08_08520)	-	1720703..1721032 (-)	330	WP_001018871.1	MTH1187 family thiamine-binding protein	-
QQO08_RS08525 (QQO08_08525)	-	1721032..1722018 (-)	987	WP_000161314.1	ROK family glucokinase	-

Sequence

Protein

• Length: 165 a.a.
• Molecular weight: 19212.92 Da
• Isoelectric Point: 10.2192

>NTDB_id=841371 QQO08_RS08485 WP_001803317.1 1716565..1717062(-) (comGF) [Staphylococcus aureus strain CC239-MRSA-III(var.) isolate Trinidad&Tobago_2020-048_8421A]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMFISITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDI
LDDFKGVDRNDIEIRQHRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide

• Length: 498 bp

>NTDB_id=841371 QQO08_RS08485 WP_001803317.1 1716565..1717062(-) (comGF) [Staphylococcus aureus strain CC239-MRSA-III(var.) isolate Trinidad&Tobago_2020-048_8421A]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGTTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGTAAAACTTTTCTAATTGAAAGTAGGGATTTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGATGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACACCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATTAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (67-155)

3D structure

• AlphaFold DB: A0A2I7Y542

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Staphylococcus aureus MW2	99.394	100	0.994
comGF	Staphylococcus aureus N315	95.758	100	0.958