Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   VT654_RS02610 Genome accession   NZ_CP142590
Coordinates   537494..538927 (-) Length   477 a.a.
NCBI ID   WP_094316476.1    Uniprot ID   -
Organism   Escherichia coli strain 64     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 532494..543927
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VT654_RS02575 (VT654_02575) elbB 533185..533838 (+) 654 WP_001299134.1 isoprenoid biosynthesis glyoxalase ElbB -
  VT654_RS02580 (VT654_02580) mtgA 533835..534563 (+) 729 WP_000047091.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  VT654_RS02585 (VT654_02585) yrbL 534560..535192 (-) 633 WP_000620405.1 PhoP regulatory network protein YrbL -
  VT654_RS02590 (VT654_02590) npr 535406..535678 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  VT654_RS02595 (VT654_02595) rapZ 535675..536529 (-) 855 WP_000243741.1 RNase adapter RapZ -
  VT654_RS02600 (VT654_02600) ptsN 536575..537066 (-) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  VT654_RS02605 (VT654_02605) hpf 537184..537471 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  VT654_RS02610 (VT654_02610) rpoN/rpoN1 537494..538927 (-) 1434 WP_094316476.1 RNA polymerase factor sigma-54 Machinery gene
  VT654_RS02615 (VT654_02615) lptB 538975..539700 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  VT654_RS02620 (VT654_02620) lptA 539707..540264 (-) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  VT654_RS02625 (VT654_02625) lptC 540233..540808 (-) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  VT654_RS02630 (VT654_02630) kdsC 540805..541371 (-) 567 WP_000030016.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  VT654_RS02635 (VT654_02635) kdsD 541392..542378 (-) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  VT654_RS02640 (VT654_02640) yrbG 542392..543369 (-) 978 WP_000922861.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54007.82 Da        Isoelectric Point: 4.3639

>NTDB_id=841284 VT654_RS02610 WP_094316476.1 537494..538927(-) (rpoN/rpoN1) [Escherichia coli strain 64]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHINTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=841284 VT654_RS02610 WP_094316476.1 537494..538927(-) (rpoN/rpoN1) [Escherichia coli strain 64]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
TGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATCGACATCGACGAGGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTAGCAGCAAAAGATCTGCGTGACTGCCTGCTAATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACATCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.024

100

0.463