Detailed information NTG0840940

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: VJW35_RS00860
• Genome accession: NZ_CP142382
• Coordinates: 182447..183430 (+)
• Length: 327 a.a.
• NCBI ID: WP_001196477.1
• Uniprot ID: A0A0H2VBW7
• Organism: Escherichia coli strain HS1496
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 177447..188430

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VJW35_RS00845 (VJW35_00845)	dppA	178591..180198 (+)	1608	WP_001222871.1	dipeptide ABC transporter substrate-binding protein DppA	-
VJW35_RS00850 (VJW35_00850)	dppB	180505..181524 (+)	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-
VJW35_RS00855 (VJW35_00855)	dppC	181534..182436 (+)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
VJW35_RS00860 (VJW35_00860)	amiE	182447..183430 (+)	984	WP_001196477.1	dipeptide ABC transporter ATP-binding protein	Regulator
VJW35_RS00865 (VJW35_00865)	dppF	183427..184431 (+)	1005	WP_000103571.1	dipeptide ABC transporter ATP-binding subunit DppF	-
VJW35_RS00870 (VJW35_00870)	yhjV	184461..185732 (-)	1272	WP_001296513.1	aromatic amino acid transport family protein	-
VJW35_RS00875 (VJW35_00875)	-	186208..186315 (+)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-
VJW35_RS00880 (VJW35_00880)	-	186691..186798 (+)	108	WP_000170745.1	type I toxin-antitoxin system toxin Ldr family protein	-
VJW35_RS00885 (VJW35_00885)	-	187174..187281 (+)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35801.38 Da
• Isoelectric Point: 6.5813

>NTDB_id=840940 VJW35_RS00860 WP_001196477.1 182447..183430(+) (amiE) [Escherichia coli strain HS1496]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNLVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDKCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=840940 VJW35_RS00860 WP_001196477.1 182447..183430(+) (amiE) [Escherichia coli strain HS1496]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCACCGTTCCGCGCTGTAGACCGCATCAG
CTACAGCGTAAAACAGGGTGAAGTGGTCGGGATTGTGGGTGAGTCCGGCTCTGGTAAATCGGTCAGTTCGCTGGCGATTA
TGGGGCTGATTGATTATCCGGGCCGCGTAATGGCGGAAAAGCTGGAGTTTAACGGTCAGGATTTGCAGCGTATCTCAGAA
AAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCAATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACAC
CGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCATCAAGGCGGCAACAAAAGTACCCGCCGTCAGCGAGCGATTGACC
TGCTGAATCTGGTCGGTATTCCCGATCCGGCATCGCGTCTGGATGTTTACCCGCATCAGCTTTCCGGCGGCATGAGCCAG
CGCGTGATGATCGCCATGGCGATTGCCTGTCGGCCAAAACTGCTGATTGCCGATGAACCAACCACTGCGCTGGACGTGAC
CATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGGCGCTGGTGTTAATTACCCATGACC
TGGCGCTGGTGGCGGAAGCGGCACATAAAATTATCGTGATGTATGCAGGCCAGGTAGTAGAAACGGGCGATGCGCACGCT
ATCTTCCATGCGCCGCGTCACCCGTATACTCAGGCATTGCTGCGTGCGCTGCCGGAATTTGCTCAGGACAAAGAACGTCT
GGCGTCGTTGCCAGGTGTCGTTCCCGGCAAGTACGACCGCCCGAACGGCTGCCTGCTTAACCCGCGCTGCCCCTACGCCA
CTGACAAATGCCGTGCTGAAGAACCGGCGCTGAATATGCTCGCTGACGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• ABC transporter (ABC_tran) (24-181)

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

3D structure

• AlphaFold DB: A0A0H2VBW7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404