Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   VPJ62_RS19135 Genome accession   NZ_CP142044
Coordinates   3843145..3843585 (+) Length   146 a.a.
NCBI ID   WP_029392818.1    Uniprot ID   -
Organism   Escherichia coli strain L9     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3838145..3848585
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VPJ62_RS19115 (VPJ62_19115) aroP 3839143..3840516 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  VPJ62_RS19120 (VPJ62_19120) ampE 3840559..3841413 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  VPJ62_RS19125 (VPJ62_19125) ampD 3841410..3841961 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  VPJ62_RS19130 (VPJ62_19130) nadC 3842049..3842942 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  VPJ62_RS19135 (VPJ62_19135) pilA 3843145..3843585 (+) 441 WP_029392818.1 prepilin peptidase-dependent pilin Machinery gene
  VPJ62_RS19140 (VPJ62_19140) pilB 3843595..3844980 (+) 1386 WP_001025145.1 type II secretion system protein GspE Machinery gene
  VPJ62_RS19145 (VPJ62_19145) hofC 3844970..3846172 (+) 1203 WP_000157266.1 protein transport protein HofC -
  VPJ62_RS19150 (VPJ62_19150) guaC 3846207..3847250 (-) 1044 WP_001217338.1 GMP reductase -
  VPJ62_RS19155 (VPJ62_19155) - 3847406..3847450 (-) 45 WP_120795372.1 protein YacM -
  VPJ62_RS19160 (VPJ62_19160) coaE 3847475..3848095 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15607.73 Da        Isoelectric Point: 4.3938

>NTDB_id=839566 VPJ62_RS19135 WP_029392818.1 3843145..3843585(+) (pilA) [Escherichia coli strain L9]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMSPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=839566 VPJ62_RS19135 WP_029392818.1 3843145..3843585(+) (pilA) [Escherichia coli strain L9]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GTCACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363