Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   JN157_RS07230 Genome accession   NZ_AP024200
Coordinates   1558744..1559718 (-) Length   324 a.a.
NCBI ID   WP_031787262.1    Uniprot ID   A0A7U7JUV7
Organism   Staphylococcus argenteus strain Tokyo13064     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1553744..1564718
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JN157_RS07190 (TMSFP064_13980) gcvT 1554244..1555335 (-) 1092 WP_047526706.1 glycine cleavage system aminomethyltransferase GcvT -
  JN157_RS07195 (TMSFP064_13990) - 1555492..1556016 (-) 525 WP_047526708.1 shikimate kinase -
  JN157_RS07200 (TMSFP064_14000) - 1556003..1556152 (-) 150 WP_072292059.1 hypothetical protein -
  JN157_RS07205 (TMSFP064_14010) comGF 1556251..1556706 (-) 456 WP_078377685.1 competence type IV pilus minor pilin ComGF Machinery gene
  JN157_RS07210 (TMSFP064_14020) comGE 1556666..1556965 (-) 300 WP_000800819.1 hypothetical protein Machinery gene
  JN157_RS07215 (TMSFP064_14030) comGD 1556952..1557398 (-) 447 WP_072292023.1 competence type IV pilus minor pilin ComGD Machinery gene
  JN157_RS07220 (TMSFP064_14040) comGC 1557376..1557687 (-) 312 WP_000921699.1 competence type IV pilus major pilin ComGC Machinery gene
  JN157_RS07225 (TMSFP064_14050) comGB 1557702..1558772 (-) 1071 WP_047434565.1 competence type IV pilus assembly protein ComGB Machinery gene
  JN157_RS07230 (TMSFP064_14060) comGA 1558744..1559718 (-) 975 WP_031787262.1 competence type IV pilus ATPase ComGA Machinery gene
  JN157_RS07235 (TMSFP064_14070) - 1559770..1560393 (-) 624 WP_001222932.1 MBL fold metallo-hydrolase -
  JN157_RS07240 (TMSFP064_14080) - 1560390..1560719 (-) 330 WP_001018874.1 MTH1187 family thiamine-binding protein -
  JN157_RS07245 (TMSFP064_14090) - 1560719..1561705 (-) 987 WP_000161316.1 ROK family glucokinase -
  JN157_RS07250 (TMSFP064_14100) - 1561705..1561905 (-) 201 WP_000087563.1 YqgQ family protein -
  JN157_RS07255 (TMSFP064_14110) - 1561886..1563349 (-) 1464 WP_047526716.1 rhomboid family protein -
  JN157_RS07260 (TMSFP064_14120) - 1563361..1563900 (-) 540 WP_072292060.1 5-formyltetrahydrofolate cyclo-ligase -
  JN157_RS07265 (TMSFP064_14130) rpmG 1564112..1564261 (-) 150 WP_001265709.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36867.03 Da        Isoelectric Point: 9.0234

>NTDB_id=83932 JN157_RS07230 WP_031787262.1 1558744..1559718(-) (comGA) [Staphylococcus argenteus strain Tokyo13064]
MKILFQEIINKAIVMKASDVHFIPVHNEVSIKFRINDNLEQYEEIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQYFKQTKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNEKAGINYVNSFKAILRCDPDVILIGEIRDKEVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLQYFFSHNHSLPTSFKKLETKLDDMTKAGVICESTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=83932 JN157_RS07230 WP_031787262.1 1558744..1559718(-) (comGA) [Staphylococcus argenteus strain Tokyo13064]
ATGAAGATTTTATTTCAAGAGATAATCAACAAAGCGATAGTAATGAAAGCGAGTGATGTCCATTTTATTCCTGTTCATAA
TGAAGTGAGTATTAAATTTCGTATAAATGACAATTTGGAACAGTATGAAGAAATTGGAAATAGTATTTATCAAAAGCTAT
TAGTTTATATGAAATTTCAAGCTGGTCTCGATGTATCTACGCAACAAGTTGCTCAGAGTGGTCGTTATAGCTACCATTTT
AATAAAATTTATTTTTTAAGAATTTCAACATTGCCATTATCACTTGGTCAAGAAAGTTGTGTGATAAGAATAGTTCCTCA
ATATTTTAAACAAACTAAATCAACATATAAATTTAATGATTTTAAACATTTAATGAATAAGAAACAAGGCTTATTGTTAT
TTAGTGGTCCGACGGGTTCAGGTAAAAGTACTTTAATGTATCAAATGGTCTCATACGCTAACAAAGCATTAAACTTAAAT
GTTATATCTATTGAAGACCCAGTAGAAATGCAAATTCCTGGAATAGTTCAAATAAACGTAAATGAAAAAGCTGGGATTAA
CTATGTCAATTCATTTAAAGCGATATTAAGGTGTGACCCAGATGTTATTCTTATAGGGGAAATTAGAGATAAAGAAGTAG
CTAAATGTGTCATCCAAGCAAGTTTAAGTGGTCATCTAGTACTCACTACCTTACATGCAACAGATTGTAAAGGTGCAATT
TTAAGATTACTAGAAATGGGGATTTCAGTACAAGAACTAATACAAGCAACTAATTTAATTATAAATCAAAGACTTGTTAC
TACAATTAAACAGCAACGACAATTAGTGTGTGAAATTTTATCACAACAACAACTTCAGTATTTCTTCTCGCATAATCATT
CATTACCTACCTCATTTAAAAAACTAGAAACAAAGCTAGATGATATGACAAAAGCAGGTGTCATTTGTGAAAGTACAATG
GATAAGTATATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7U7JUV7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

95.988

100

0.96
  comGA Staphylococcus aureus N315

95.988

100

0.96

Multiple sequence alignment