Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   QPL78_RS13270 Genome accession   NZ_CP126530
Coordinates   2574376..2574801 (-) Length   141 a.a.
NCBI ID   WP_101864562.1    Uniprot ID   -
Organism   Bacillus halotolerans strain Tehuacan_S4     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2569376..2579801
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QPL78_RS13240 (QPL78_13240) - 2569612..2569746 (-) 135 WP_284559343.1 hypothetical protein -
  QPL78_RS13245 (QPL78_13245) - 2569827..2570558 (-) 732 WP_101864558.1 SGNH/GDSL hydrolase family protein -
  QPL78_RS13250 (QPL78_13250) - 2570805..2571557 (-) 753 WP_284559344.1 N-acetylmuramoyl-L-alanine amidase -
  QPL78_RS13255 (QPL78_13255) - 2571752..2572378 (+) 627 WP_284559347.1 TVP38/TMEM64 family protein -
  QPL78_RS13260 (QPL78_13260) gnd 2572400..2573293 (-) 894 WP_101864560.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  QPL78_RS13265 (QPL78_13265) - 2573614..2574342 (+) 729 WP_284559353.1 DUF308 domain-containing protein -
  QPL78_RS13270 (QPL78_13270) nucA/comI 2574376..2574801 (-) 426 WP_101864562.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  QPL78_RS13275 (QPL78_13275) sigK 2574978..2575706 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  QPL78_RS13280 (QPL78_13280) fumC 2576280..2577668 (-) 1389 WP_284559358.1 class II fumarate hydratase -
  QPL78_RS13285 (QPL78_13285) - 2577835..2578725 (+) 891 WP_284559359.1 LysR family transcriptional regulator -
  QPL78_RS13290 (QPL78_13290) - 2579145..2579309 (-) 165 Protein_2567 recombinase family protein -
  QPL78_RS13295 (QPL78_13295) - 2579267..2579445 (-) 179 Protein_2568 hypothetical protein -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15576.73 Da        Isoelectric Point: 5.2215

>NTDB_id=838500 QPL78_RS13270 WP_101864562.1 2574376..2574801(-) (nucA/comI) [Bacillus halotolerans strain Tehuacan_S4]
MKRWMAMFLLAAAVFLCLLGKDMTAPQQVKGAALYDKVLYFPLSRYPETGDHIRDAIAEGHSDICTIDRDGADKRREESL
KGIPTKPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTKVLFIVQ

Nucleotide


Download         Length: 426 bp        

>NTDB_id=838500 QPL78_RS13270 WP_101864562.1 2574376..2574801(-) (nucA/comI) [Bacillus halotolerans strain Tehuacan_S4]
ATGAAAAGATGGATGGCAATGTTTCTTCTGGCGGCAGCAGTTTTTCTTTGTTTGTTGGGAAAGGATATGACGGCTCCGCA
ACAGGTCAAGGGGGCTGCTTTATATGACAAAGTTTTATATTTTCCGTTGTCTCGTTATCCGGAAACCGGGGATCATATAA
GAGATGCGATTGCCGAAGGACATTCTGATATATGTACAATTGATCGTGATGGAGCAGACAAAAGGCGGGAGGAATCATTA
AAAGGAATTCCGACGAAGCCTGGCTATGATCGGGATGAATGGCCGATGGCAGTTTGTGAAGAAGGTGGCGCCGGAGCCGA
TGTCCGATATGTAACGCCTTCTGATAATCGCGGCGCCGGTTCATGGGTAGGAAATCAAATGAGCAGTTATCCAGATGGCA
CCAAAGTGTTGTTTATTGTTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(45-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

61.29

87.943

0.539