Detailed information NTG0838440

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: QPL84_RS25485
• Genome accession: NZ_CP126525
• Coordinates: 4923022..4924371 (-)
• Length: 449 a.a.
• NCBI ID: WP_185778120.1
• Uniprot ID: -
• Organism: Bacillus toyonensis strain Puebla_S2
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4918022..4929371

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QPL84_RS25465 (QPL84_25465)	secA	4918351..4920858 (-)	2508	WP_000579396.1	preprotein translocase subunit SecA	-
QPL84_RS25470 (QPL84_25470)	raiA	4921133..4921675 (-)	543	WP_000671188.1	ribosome-associated translation inhibitor RaiA	-
QPL84_RS25475 (QPL84_25475)	cspC	4921994..4922191 (-)	198	WP_002045823.1	cold shock protein CspC	-
QPL84_RS25480 (QPL84_25480)	-	4922318..4923022 (-)	705	WP_000543520.1	ComF family protein	-
QPL84_RS25485 (QPL84_25485)	comFA	4923022..4924371 (-)	1350	WP_185778120.1	ATP-dependent helicase ComFA	Machinery gene
QPL84_RS25490 (QPL84_25490)	-	4924498..4925895 (-)	1398	WP_141555820.1	C40 family peptidase	-
QPL84_RS25495 (QPL84_25495)	-	4926056..4926370 (-)	315	WP_000400852.1	helix-turn-helix domain-containing protein	-
QPL84_RS25500 (QPL84_25500)	-	4926542..4927384 (-)	843	WP_000844774.1	DegV family protein	-
QPL84_RS25505 (QPL84_25505)	-	4927623..4928258 (+)	636	WP_000926676.1	YigZ family protein	-

Sequence

Protein

• Length: 449 a.a.
• Molecular weight: 50890.66 Da
• Isoelectric Point: 9.5194

>NTDB_id=838440 QPL84_RS25485 WP_185778120.1 4923022..4924371(-) (comFA) [Bacillus toyonensis strain Puebla_S2]
MLSGKQLLLEELSTDVRDNLDDLKKKGEVVCVQGVKNKASTYMCQRCGNIAQRLFSSFLCKRCSKVCTYCRKCITMGRVS
ECAVLVRGIAEKKGEMDVNPLQWKGNLSTGQELAAQGVMEAVKQKESFFIWAVCGAGKTEMLFYGIAEALQKGERVCIAT
PRTDVVLELAPRLKEVFPNINVAALYGGSLDREKDAALVVATTHQLLRYYRAFHVMIVDEIDAFPYHADQMLQYAVKQAM
KEKAACIYLTATPDEEWKRNLRKGKQKGIVVSGRYHRHPLPVPKFCWCGNWKKSLINKRIPQVLLQWLKVYLNKKHPIFL
FVPHVRYIEEMSLLLKLLDDRVAGVHAEDPMRKEKVAAFRKGEIPLLVTTTILERGVTVKNLQVAVLGTEEEIFSESALV
QIAGRVGRSFEEPYGEVIYFHYGKTEAMVRAKKHIRSMNKNAKEQGLID

Nucleotide

• Length: 1350 bp

>NTDB_id=838440 QPL84_RS25485 WP_185778120.1 4923022..4924371(-) (comFA) [Bacillus toyonensis strain Puebla_S2]
ATGTTATCTGGGAAACAGTTATTATTAGAAGAACTCTCTACAGACGTACGGGATAATTTAGATGATTTGAAGAAGAAGGG
AGAGGTCGTATGTGTACAAGGTGTAAAAAATAAGGCTTCTACATATATGTGTCAGCGTTGCGGAAATATAGCACAAAGAC
TCTTTTCATCATTTTTGTGTAAAAGGTGTAGTAAAGTCTGCACATATTGCCGGAAGTGCATAACGATGGGGAGAGTTAGT
GAATGTGCTGTACTTGTTCGTGGAATTGCTGAAAAAAAGGGAGAAATGGATGTAAACCCGTTACAGTGGAAAGGAAATTT
ATCTACTGGCCAGGAGTTGGCTGCGCAAGGTGTTATGGAGGCTGTTAAACAGAAAGAATCGTTTTTTATTTGGGCTGTGT
GCGGCGCTGGAAAAACAGAAATGTTGTTTTATGGTATTGCAGAGGCACTTCAAAAAGGAGAAAGAGTTTGTATCGCAACG
CCGAGAACGGATGTTGTACTTGAATTAGCACCGAGATTGAAAGAAGTATTTCCGAATATAAATGTAGCTGCTTTATACGG
TGGGAGTTTAGATCGGGAAAAAGATGCAGCGTTAGTCGTTGCGACCACCCATCAGCTATTGCGTTATTATAGAGCGTTTC
ATGTCATGATTGTAGATGAAATAGATGCCTTTCCATACCATGCAGATCAAATGTTGCAGTATGCGGTAAAACAAGCGATG
AAAGAGAAGGCGGCATGTATTTATTTAACTGCAACTCCAGATGAAGAGTGGAAGCGTAACCTTAGAAAGGGGAAACAAAA
AGGCATCGTTGTTTCTGGACGGTATCACCGTCATCCGTTGCCAGTCCCCAAATTTTGCTGGTGCGGAAACTGGAAGAAAA
GCCTCATCAATAAAAGAATTCCTCAAGTTTTACTACAATGGTTAAAAGTATACTTAAATAAAAAGCACCCCATTTTTTTA
TTTGTTCCCCATGTGCGATATATAGAAGAAATGAGTCTGTTATTGAAGTTGTTAGACGATAGAGTTGCCGGTGTACATGC
AGAAGATCCAATGAGAAAAGAGAAAGTTGCAGCTTTCAGAAAGGGAGAAATTCCTTTATTAGTTACAACGACGATTTTGG
AGAGAGGAGTAACGGTGAAAAATTTGCAAGTTGCGGTTTTAGGGACGGAAGAAGAAATATTTTCAGAAAGCGCACTCGTA
CAAATTGCAGGCCGGGTAGGTCGTAGTTTTGAAGAGCCGTATGGAGAGGTCATTTATTTTCATTATGGTAAGACAGAGGC
GATGGTGCGCGCGAAAAAACACATTCGAAGTATGAACAAAAATGCGAAAGAACAAGGGTTAATCGATTAA

Domains

Predicted by InterproScan.

• Helicase conserved C-terminal domain (Helicase_C) (313-408)

• Type III restriction enzyme, res subunit (ResIII) (106-254)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Bacillus subtilis subsp. subtilis str. 168	50.226	98.441	0.494
comFA	Latilactobacillus sakei subsp. sakei 23K	40.199	89.755	0.361