Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   QPL84_RS20960 Genome accession   NZ_CP126525
Coordinates   4086301..4087344 (-) Length   347 a.a.
NCBI ID   WP_001013172.1    Uniprot ID   -
Organism   Bacillus toyonensis strain Puebla_S2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4081301..4092344
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QPL84_RS20915 (QPL84_20915) - 4081726..4081926 (-) 201 WP_000106086.1 YqzE family protein -
  QPL84_RS20920 (QPL84_20920) - 4081965..4082462 (-) 498 WP_000836652.1 shikimate kinase -
  QPL84_RS20925 (QPL84_20925) - 4082583..4083233 (-) 651 WP_000183872.1 2OG-Fe(II) oxygenase -
  QPL84_RS20930 (QPL84_20930) comGG 4083408..4083779 (-) 372 WP_000743702.1 competence type IV pilus minor pilin ComGG -
  QPL84_RS20935 (QPL84_20935) comGF 4083776..4084249 (-) 474 WP_000477944.1 competence type IV pilus minor pilin ComGF -
  QPL84_RS20940 (QPL84_20940) - 4084219..4084521 (-) 303 WP_000829524.1 hypothetical protein -
  QPL84_RS20945 (QPL84_20945) comGD 4084514..4084969 (-) 456 WP_000810404.1 competence type IV pilus minor pilin ComGD -
  QPL84_RS20950 (QPL84_20950) comGC 4084966..4085265 (-) 300 WP_001178691.1 competence type IV pilus major pilin ComGC -
  QPL84_RS20955 (QPL84_20955) comGB 4085277..4086314 (-) 1038 WP_284558167.1 competence type IV pilus assembly protein ComGB -
  QPL84_RS20960 (QPL84_20960) comGA 4086301..4087344 (-) 1044 WP_001013172.1 competence type IV pilus ATPase ComGA Machinery gene
  QPL84_RS20965 (QPL84_20965) - 4087549..4088244 (+) 696 WP_000434112.1 helix-turn-helix domain-containing protein -
  QPL84_RS20970 (QPL84_20970) - 4088370..4088612 (+) 243 WP_000440717.1 DUF2626 domain-containing protein -
  QPL84_RS20975 (QPL84_20975) - 4088720..4090114 (+) 1395 WP_284558168.1 L-cystine transporter -
  QPL84_RS20980 (QPL84_20980) - 4090177..4090569 (-) 393 WP_000875407.1 hypothetical protein -
  QPL84_RS20985 (QPL84_20985) - 4090657..4090872 (-) 216 WP_001008324.1 DUF3912 family protein -
  QPL84_RS20990 (QPL84_20990) - 4091125..4091436 (+) 312 WP_001092833.1 hypothetical protein -
  QPL84_RS20995 (QPL84_20995) - 4091472..4091960 (-) 489 WP_000764243.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39204.57 Da        Isoelectric Point: 8.5491

>NTDB_id=838429 QPL84_RS20960 WP_001013172.1 4086301..4087344(-) (comGA) [Bacillus toyonensis strain Puebla_S2]
MNGIEIFANTILKEACRVQASDLHIVPRQKDVAVQLRIGKDLITKQCIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQIDGQEVYLRLSTLPTVYRESLVIRLHLQASVQPLSHLSLFPSTAEKLLSFLRYSHGLLVFTGPTGSGKTTTMYALLEV
IRKRKTRRIVTLEDPVEKRKDDLLQIQINEKAGITYEAGLKAILRHDPDIILVGEIRDEETAKIAVRASLTGHLVMTTLH
TNDAKGAILRFMDYGITRQEIEQALLAVAAQRLVELKCPFCRGKCSILCKSMRQVRQASVYELLDGYDLQQAIKEANGEC
VTYKHETLESSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=838429 QPL84_RS20960 WP_001013172.1 4086301..4087344(-) (comGA) [Bacillus toyonensis strain Puebla_S2]
ATGAATGGAATTGAAATTTTTGCGAATACAATTTTGAAAGAAGCGTGTAGGGTACAAGCATCGGACTTACATATTGTGCC
CCGGCAGAAGGATGTAGCGGTTCAGCTACGTATAGGAAAAGATTTAATTACGAAACAATGTATTGAAAAGGAGTTTGGAG
AAAAACTTGTTTCGCACTTTAAATTTTTGGCATCCATGGATATAGGAGAGAGGCGGAAGCCACAAAATGGTTCATTGTAT
TTACAAATTGATGGACAAGAAGTGTATTTACGTCTTTCAACACTTCCGACTGTATATCGAGAAAGTCTCGTGATTCGCCT
TCATTTACAAGCATCTGTGCAGCCATTATCTCATCTTTCGTTATTTCCAAGTACGGCGGAAAAACTCCTCTCTTTTTTAC
GTTATTCACATGGTCTACTCGTATTTACTGGACCGACTGGTTCTGGGAAGACAACGACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAGGAAAACACGTCGAATTGTAACACTTGAAGACCCAGTTGAAAAAAGAAAAGACGATTTATTACAAATTCA
AATAAATGAAAAAGCAGGTATCACATATGAGGCTGGACTAAAGGCTATTTTACGCCATGATCCAGATATTATATTAGTTG
GTGAAATCCGGGATGAAGAAACAGCAAAAATAGCTGTAAGAGCAAGTTTAACTGGACATTTAGTAATGACGACCCTGCAT
ACGAATGATGCGAAAGGAGCGATACTCAGATTTATGGATTATGGCATAACGAGGCAAGAAATTGAACAAGCTTTATTGGC
TGTAGCTGCACAGCGACTTGTTGAATTAAAGTGTCCATTTTGCAGAGGAAAGTGCTCAATTTTATGTAAATCAATGCGGC
AAGTAAGACAAGCAAGCGTTTACGAGTTGTTAGATGGATATGATTTGCAACAAGCAATTAAAGAAGCGAACGGAGAATGT
GTTACGTACAAACATGAAACATTAGAATCTTCGATACGAAAAGGGTATGCTTTAGGATTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.637

100

0.576