Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   QPL77_RS11555 Genome accession   NZ_CP126521
Coordinates   2294718..2295488 (-) Length   256 a.a.
NCBI ID   WP_012010612.1    Uniprot ID   A8FFB2
Organism   Bacillus pumilus strain Monterrey_S2     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2289718..2300488
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QPL77_RS11535 (QPL77_11535) - 2289811..2290632 (-) 822 WP_211063022.1 pyruvate, water dikinase regulatory protein -
  QPL77_RS11540 (QPL77_11540) - 2290658..2291296 (-) 639 WP_024423017.1 helix-turn-helix transcriptional regulator -
  QPL77_RS11545 (QPL77_11545) glyS 2291463..2293508 (-) 2046 WP_211063021.1 glycine--tRNA ligase subunit beta -
  QPL77_RS11550 (QPL77_11550) glyQ 2293501..2294388 (-) 888 WP_003217487.1 glycine--tRNA ligase subunit alpha -
  QPL77_RS11555 (QPL77_11555) recO 2294718..2295488 (-) 771 WP_012010612.1 DNA repair protein RecO Machinery gene
  QPL77_RS11560 (QPL77_11560) - 2295525..2295668 (-) 144 WP_003217288.1 YqzL family protein -
  QPL77_RS11565 (QPL77_11565) era 2295817..2296722 (-) 906 WP_088000408.1 GTPase Era -
  QPL77_RS11570 (QPL77_11570) - 2296715..2297113 (-) 399 WP_144481943.1 cytidine deaminase -
  QPL77_RS11575 (QPL77_11575) - 2297227..2297613 (-) 387 WP_144522100.1 diacylglycerol kinase family protein -
  QPL77_RS11580 (QPL77_11580) ybeY 2297591..2298070 (-) 480 WP_144522102.1 rRNA maturation RNase YbeY -
  QPL77_RS11585 (QPL77_11585) - 2298071..2300158 (-) 2088 WP_186325045.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 256 a.a.        Molecular weight: 29230.90 Da        Isoelectric Point: 8.4348

>NTDB_id=838323 QPL77_RS11555 WP_012010612.1 2294718..2295488(-) (recO) [Bacillus pumilus strain Monterrey_S2]
MLIKSEGIVLRTTDYGETNKIVTLLTREHGKIGVMARGAKKSNSRLSAISQPFLYGTFLIQSSTGLGTLQQGEMIESMRT
IREDLFLTAYAAYMTELLDKGTEEKKPNPYLFELLLQSLRHLNEGTDADIILFIVEVKMLSVMGMKPELDQCVHCGQKEG
QFHFSIRDNGFICQNCFSKDPYKLPLSPAAARLLRLFHYFDLSRLGQVDVKPQTKQEIRQVLDHYYDEYSGLYLKSKKFM
NQMESMKKLMGGENKS

Nucleotide


Download         Length: 771 bp        

>NTDB_id=838323 QPL77_RS11555 WP_012010612.1 2294718..2295488(-) (recO) [Bacillus pumilus strain Monterrey_S2]
ATGCTCATTAAAAGTGAAGGAATCGTCCTTAGAACAACAGATTATGGCGAAACAAACAAAATTGTCACGCTGCTCACTAG
AGAACATGGGAAAATTGGTGTGATGGCAAGAGGCGCAAAAAAATCAAACAGCCGTCTTTCAGCCATAAGCCAGCCGTTTT
TATACGGAACCTTCTTGATCCAATCATCAACAGGACTTGGAACGCTCCAGCAAGGTGAAATGATTGAAAGTATGCGGACC
ATTCGGGAAGATTTATTTTTGACCGCATATGCCGCATACATGACAGAGTTACTCGATAAAGGCACAGAAGAAAAAAAGCC
AAACCCTTATTTGTTTGAGCTCCTGCTTCAATCTCTGCGTCATCTGAATGAAGGCACAGATGCTGATATTATCTTATTTA
TCGTAGAGGTTAAAATGTTATCAGTGATGGGGATGAAGCCCGAACTTGATCAATGTGTTCATTGTGGTCAAAAAGAAGGG
CAGTTTCATTTCTCAATTAGAGATAACGGCTTCATTTGCCAAAATTGCTTTTCTAAAGACCCGTATAAACTGCCCTTATC
CCCAGCAGCTGCAAGGCTGCTCCGTTTATTTCATTATTTTGATTTATCAAGGCTCGGTCAGGTTGATGTGAAACCGCAGA
CGAAACAAGAAATTCGTCAAGTGCTCGATCACTACTATGACGAGTATTCAGGCCTTTATTTGAAATCAAAAAAATTCATG
AATCAAATGGAATCCATGAAAAAGCTCATGGGCGGGGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-77)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A8FFB2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

75.391

100

0.754