Detailed information    

insolico Bioinformatically predicted

Overview

Name   recU   Type   Machinery gene
Locus tag   QPL77_RS10065 Genome accession   NZ_CP126521
Coordinates   2027843..2028454 (+) Length   203 a.a.
NCBI ID   WP_144521293.1    Uniprot ID   -
Organism   Bacillus pumilus strain Monterrey_S2     
Function   plasmid transformation; homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2022843..2033454
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QPL77_RS10025 (QPL77_10025) - 2023010..2024128 (+) 1119 WP_284553845.1 NADH-dependent flavin oxidoreductase -
  QPL77_RS10030 (QPL77_10030) - 2024179..2024685 (-) 507 WP_211064267.1 PTS glucose transporter subunit IIA -
  QPL77_RS10035 (QPL77_10035) - 2024854..2025273 (+) 420 WP_144521281.1 Hsp20/alpha crystallin family protein -
  QPL77_RS10040 (QPL77_10040) - 2025294..2025746 (-) 453 WP_211064266.1 YppG family protein -
  QPL77_RS10045 (QPL77_10045) - 2025853..2026224 (-) 372 WP_088001031.1 YppE family protein -
  QPL77_RS10050 (QPL77_10050) - 2026282..2026515 (-) 234 WP_249407480.1 DUF5446 family protein -
  QPL77_RS10055 (QPL77_10055) sspM 2026712..2026816 (+) 105 WP_012010346.1 acid-soluble spore protein SspM -
  QPL77_RS10060 (QPL77_10060) - 2026840..2027796 (-) 957 WP_144521278.1 DUF2515 domain-containing protein -
  QPL77_RS10065 (QPL77_10065) recU 2027843..2028454 (+) 612 WP_144521293.1 Holliday junction resolvase RecU Machinery gene
  QPL77_RS10070 (QPL77_10070) - 2028498..2031218 (+) 2721 WP_211064265.1 PBP1A family penicillin-binding protein -
  QPL77_RS10075 (QPL77_10075) - 2031254..2031763 (-) 510 WP_211064264.1 YpoC family protein -
  QPL77_RS10080 (QPL77_10080) nth 2031760..2032422 (-) 663 WP_211064263.1 endonuclease III -
  QPL77_RS10085 (QPL77_10085) - 2032440..2033144 (-) 705 WP_144521274.1 DnaD domain-containing protein -

Sequence

Protein


Download         Length: 203 a.a.        Molecular weight: 23301.52 Da        Isoelectric Point: 9.4107

>NTDB_id=838317 QPL77_RS10065 WP_144521293.1 2027843..2028454(+) (recU) [Bacillus pumilus strain Monterrey_S2]
MIRYPNGKSYQPIQPIGTKKRISGESSYSNRGMTLEADLNETNQYYLVNGIAVIHKKPTPVQIVNVDYPKRSAAVIKEAY
FKQSSTTDYNGVYKGRYIDFEAKETKSTTSFPLKNFHDHQIEHMKQVEKQGGICFVIISAFGSVYYLPASDLFFFWERQK
LNGRKSISKDELMEAGHLMTLGYSPRIDYIKVVETLHFSDESK

Nucleotide


Download         Length: 612 bp        

>NTDB_id=838317 QPL77_RS10065 WP_144521293.1 2027843..2028454(+) (recU) [Bacillus pumilus strain Monterrey_S2]
ATGATTCGGTATCCAAACGGAAAATCGTATCAGCCCATTCAACCAATTGGGACAAAAAAACGAATCAGCGGCGAATCCTC
TTATAGTAACCGCGGGATGACGCTTGAGGCTGACTTAAACGAAACCAACCAGTACTATTTGGTCAACGGGATCGCAGTCA
TTCATAAGAAGCCTACCCCCGTTCAAATTGTGAACGTGGATTATCCAAAAAGAAGCGCTGCTGTGATCAAAGAAGCCTAT
TTTAAACAATCATCCACAACAGACTATAATGGAGTGTACAAAGGGCGCTATATCGATTTTGAAGCAAAGGAAACCAAAAG
CACCACCTCGTTTCCGCTCAAAAATTTTCATGACCATCAAATTGAGCATATGAAACAGGTTGAAAAGCAAGGCGGTATTT
GTTTCGTTATTATATCCGCATTTGGCTCTGTTTATTATTTGCCCGCGTCAGATCTCTTTTTCTTCTGGGAACGGCAGAAG
CTAAACGGCCGGAAATCCATTAGTAAAGATGAACTAATGGAAGCCGGTCACTTAATGACACTTGGATATTCGCCAAGAAT
TGATTATATTAAAGTAGTGGAGACACTTCATTTTTCGGATGAAAGTAAGTAA

Domains

Predicted by InterproScan.

Recombination protein U (RecU)

(31-193)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recU Bacillus subtilis subsp. subtilis str. 168

72.772

99.507

0.724