Detailed information NTG0838249

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: QPL81_RS07900
• Genome accession: NZ_CP126520
• Coordinates: 1092904..1096629 (+)
• Length: 1241 a.a.
• NCBI ID: WP_284557316.1
• Uniprot ID: -
• Organism: Bacillus toyonensis strain Cuernavaca_S4
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1087904..1101629

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QPL81_RS07885 (QPL81_07885)	-	1087936..1088529 (+)	594	WP_284557315.1	TVP38/TMEM64 family protein	-
QPL81_RS07890 (QPL81_07890)	lepB	1088712..1089275 (+)	564	WP_000751914.1	signal peptidase I	-
QPL81_RS07895 (QPL81_07895)	addB	1089392..1092907 (+)	3516	WP_000058642.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
QPL81_RS07900 (QPL81_07900)	addA	1092904..1096629 (+)	3726	WP_284557316.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
QPL81_RS07905 (QPL81_07905)	-	1096642..1096929 (+)	288	WP_000179959.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
QPL81_RS07910 (QPL81_07910)	gerPF	1097018..1097233 (-)	216	WP_001141566.1	spore germination protein GerPF	-
QPL81_RS07915 (QPL81_07915)	-	1097276..1097662 (-)	387	WP_000902321.1	spore germination protein GerPE	-
QPL81_RS07920 (QPL81_07920)	gerPD	1097678..1097872 (-)	195	WP_001052804.1	spore germination protein GerPD	-
QPL81_RS07925 (QPL81_07925)	gerPC	1097879..1098493 (-)	615	WP_001070753.1	spore germination protein GerPC	-
QPL81_RS07930 (QPL81_07930)	gerPB	1098562..1098768 (-)	207	WP_001012514.1	spore germination protein GerPB	-
QPL81_RS07935 (QPL81_07935)	gerPA	1098783..1099004 (-)	222	WP_001111187.1	spore germination protein GerPA	-
QPL81_RS07940 (QPL81_07940)	-	1099104..1099280 (-)	177	WP_000462849.1	aspartyl-phosphate phosphatase Spo0E family protein	-
QPL81_RS07945 (QPL81_07945)	-	1099527..1100426 (+)	900	WP_001213082.1	fumarylacetoacetate hydrolase family protein	-
QPL81_RS07950 (QPL81_07950)	-	1100462..1100743 (-)	282	WP_000926856.1	hypothetical protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142810.97 Da
• Isoelectric Point: 4.8030

>NTDB_id=838249 QPL81_RS07900 WP_284557316.1 1092904..1096629(+) (addA) [Bacillus toyonensis strain Cuernavaca_S4]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERMIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHVRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNSIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVRFQLETAEQHI
RKATELAMLPDGPAPRIETLQADAALLGMLSSAARESWTSVYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRKPESFLRDFQDMHPVLEKLVKLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSEDGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGSGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYPEGEDVAAELLCIQQTEEEVLEGEEGTEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEQELHEKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEILLELGQGSIPDEIYGYDTSWK
VEVVDGNTLLAPEPVQEEKQELLEALREKKAVPLQSERKEEVYDRLMWKYGYEEATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHTVMQHVDLKKPITVEVLQEQIAGMVNKELLTFEQAEEIAIEKIISFFDS
DLGKRVLAAKSVEREVPFTMMLSAEETYQDWQGNSGESILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFEQAKPIL
EDRYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNYVVNIEE

Nucleotide

• Length: 3726 bp

>NTDB_id=838249 QPL81_RS07900 WP_284557316.1 1092904..1096629(+) (addA) [Bacillus toyonensis strain Cuernavaca_S4]
ATGATAGAGAATTGGCCTAAAAAACCAGAAGGTAGTCAATGGACAGATGACCAGTGGAAAGCCGTTGTAGCGAATGGACG
TGACATTTTAGTCGCAGCAGCAGCTGGATCGGGAAAAACAGCAGTATTAGTTGAACGTATGATTAAAAAGATTATTAATG
AGGAAAATCCAGTCGATGTCGATCGCCTGCTCGTTGTAACATTTACGAATGCAGCGGCGCAAGAGATGAAAAATCGAATT
GGAGAAGCGTTAGAAAAAGTACTAATTGATGAGCCAGGATCTCAACATGTAAGAAAACAGCTGAGCCTATTAAATAAAGC
ATCTATTTCAACGATCCATTCATTTTGTTTACAAGTAATTAGAGGGTATTACTACATGCTGGATGTTGATCCTCGTTTTC
GTATTGCAAATCAAACAGAAAATGAGTTGTTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAGTATGGAATCGAA
GATAATAGTATTTTCTTTGAATTAGTTGATCGCTATACGAGTGACCGTAGTGATGATGACTTACAAAGAATGATTTTAGC
GCTTCATACAGAATCAAGAGCGCATCCAAATCCGGAAAAATGGCTCGATAAATTAGTAGAAGCATACGACGTGGAAGGAA
AGACAATTGAAGATTTAGTGTATGCTTCTTACCTATTAGAAGATGTGAGATTCCAGCTTGAAACAGCGGAACAACATATT
CGTAAAGCAACCGAACTCGCAATGCTTCCTGACGGCCCGGCGCCTCGCATTGAAACCCTGCAAGCGGATGCAGCTTTACT
TGGAATGTTATCATCAGCAGCTCGTGAATCGTGGACAAGCGTGTATGAAGCGATGCAAAACGTATCGTGGCAAACGTTAA
AGCGTATTAAGAAAAGTGATTACAACGAGGATGTTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAAGTG
AAGAAATTACAAGAAGAGTTATTTAGCCGCAAACCTGAAAGTTTCTTACGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAACTCGTGAAGCTCGTAAAAGTCTTTACAGAGCGTTTCCAAGCGATGAAGCGAGATAAAGGAATGGTTGATTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGTGAACAAAGTGAAGACGGTGAAATGAAGCCGTCCGCAGTAGCGCTTCAA
TATCGTAATAAATTTGCTGAGGTATTAGTCGATGAATATCAAGATACGAACTTCGTACAGGAATCGATTATTAAATTTGT
AACGAAAGATTCTGAGAGTGAAGGGAACTTGTTCATGGTTGGTGACGTAAAACAGTCAATCTACCGCTTCCGACTAGCAG
AACCAGGCTTATTCTTAGGAAAGTATAAACGCTTCACTCAAGAAGGATCGGGCGGCGGAATGAAGATTGATTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTACTAGCAGGTACGAACTTTATTTTTAAACAAATTATGGGCGAAGAAGTTGGAGAGAT
TGACTACGACGCTGACGCTGAATTAAAACTAGGCGCTAGTTATCCAGAAGGTGAAGATGTAGCGGCTGAATTATTATGCA
TTCAGCAAACGGAAGAAGAAGTGCTAGAGGGTGAAGAAGGTACGGAAGTCGAAAAGGCGCAACTGGAAGCTCGTCTTATG
GCGCAGCGCATTAAAGCGATGGTCGATTCAGGTTATGAAGTGTATGACCGAAAAACGGATAGTATGCGACCAGTGCAATA
TCGTGATTTCGTTATTTTACTTCGCTCTATGCCGTGGGCACCGCAAATTATGGAAGAGTTAAAACTACAAGGAATTCCAG
TATATGCAGACCTTGCGACTGGTTACTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGCGTTATTGATAAT
CCGATGCAAGATATTCCACTTGCAGCAGTACTTCGTTCACCGATCGTTGGATTAAATGATGAAGAACTTGCGACGCTTCG
TGCTCACGGAAAGAAAGGGTCATTTTATGAAGTAATGAGCTCATTCTTAAAAGGTGCACCGCTTGAAGAAGAACAAGAAC
TTCATGAAAAACTAGAATGGTTTTATAACTTACTGCAAGGATGGCGTGAATTCGCGCGCCAACAATCACTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGGTATTATGACTTTGTCGGCGGTTTACCAGCTGGAAAGCAAAGGCAGGCAAACTT
GCGTGTATTATATGACCGCGCAAGGCAATATGAAGCAACATCATTTAGAGGATTATTCCGCTTCTTACGTTTTATTGAAC
GTATTTTAGAACGCGGTGACGATATGGGGACGGCGAGAGCTCTTGGTGAACAAGAAGATGTTGTTCGCATTATGACGATT
CATAAAAGTAAGGGACTTGAGTTCCCGGTCGTATTTGTAGCTGGACTCGGTCGTCGTTTTAATACACAAGATTTAATGAA
ACGTTTCTTACTGCATAAAGACTTCGGTTTCGGTTCACAGTTCATTGATCCGCGTAAACGAATTAAATATACGACATTAT
CGCAACTTGCAATTAAACGTAAAATGAAAATGGAATTAATTGCGGAAGAAATGCGCGTATTATACGTAGCTTTAACGCGT
GCAAAAGAGAAGTTAATTTTAATCGGAACAGTTAAGGATGCAAATAAGGAAATGGAAAAATGGCTCGATGCGAGGGAACA
TAGTGAATGGTTATTACCAGATCACATACGTGCTGGAGCGTCTTGCTATTTAGACTGGATTGCACCTTCATTATATAGAC
ATCGTGATAGTGAAATACTTCTTGAATTAGGACAAGGAAGTATTCCAGATGAAATTTACGGGTATGACACTAGCTGGAAA
GTAGAAGTTGTGGACGGTAACACGCTACTTGCACCAGAGCCAGTTCAAGAAGAGAAACAAGAGTTGTTAGAAGCACTTCG
TGAGAAAAAGGCTGTGCCATTACAAAGTGAACGAAAAGAAGAAGTGTACGATAGGTTAATGTGGAAGTACGGGTATGAGG
AAGCAACCTCTCATCGTGCGAAGCAATCCGTTACAGAAATAAAGAGAAATTATCAGTCTGAAGAAGGCAGCGATAACGCC
TTTATTAAAAAACTACGTGCACCAATTAAAACACGTCCGCGCTTTATGGAGAAAAAAGGATTAACGTACGCAGAGCGTGG
GACGGCAGTCCATACCGTTATGCAACATGTAGATTTGAAGAAGCCGATTACGGTTGAAGTTCTTCAAGAACAAATTGCTG
GAATGGTAAATAAGGAATTGTTAACATTCGAGCAGGCGGAAGAAATAGCGATTGAAAAAATAATTTCATTCTTTGACAGT
GACCTAGGTAAAAGGGTATTAGCGGCGAAAAGTGTTGAGCGTGAAGTTCCATTTACGATGATGCTATCAGCAGAAGAAAC
ATATCAAGATTGGCAAGGGAATAGCGGGGAATCGATTCTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAAGAAGATG
GTATTACGCTAATCGATTTCAAAACAGATACGATTGAAGGTAAATTCCCAGGCGGATTTGAACAAGCAAAACCAATTTTA
GAAGATCGCTATAAAGTGCAGCTTTCGTTATATGCAAAAGCACTCGAGAAAAGCTTACAACATCCTGTGAAAGAGAAATG
TTTATACTTCTTTGATGGGAATTATGTTGTGAATATTGAGGAATAG

Domains

Predicted by InterproScan.

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1060-1231)

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.408	100	0.537