Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   VB057_RS01945 Genome accession   NZ_CP141791
Coordinates   423824..424501 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain BHD6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 418824..429501
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VB057_RS01930 (VB057_01930) chiA 420191..422884 (+) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -
  VB057_RS01935 (VB057_01935) bfd 423053..423247 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  VB057_RS01940 (VB057_01940) bfr 423319..423795 (+) 477 WP_000675504.1 bacterioferritin -
  VB057_RS01945 (VB057_01945) pilD 423824..424501 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  VB057_RS01950 (VB057_01950) gspM 424501..424962 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  VB057_RS01955 (VB057_01955) gspL 424959..426122 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  VB057_RS01960 (VB057_01960) gspK 426137..427120 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  VB057_RS01965 (VB057_01965) gspJ 427113..427700 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  VB057_RS01970 (VB057_01970) gspI 427693..428070 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  VB057_RS01975 (VB057_01975) gspH 428067..428576 (-) 510 WP_024238847.1 type II secretion system minor pseudopilin GspH -
  VB057_RS01980 (VB057_01980) gspG 428584..429021 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=838065 VB057_RS01945 WP_000178161.1 423824..424501(-) (pilD) [Escherichia coli strain BHD6]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=838065 VB057_RS01945 WP_000178161.1 423824..424501(-) (pilD) [Escherichia coli strain BHD6]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378