Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   U8328_RS02610 Genome accession   NZ_CP141767
Coordinates   552579..554012 (-) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli strain T-HYM1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 547579..559012
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U8328_RS02575 (U8328_02575) elbB 548271..548924 (+) 654 WP_001300411.1 isoprenoid biosynthesis glyoxalase ElbB -
  U8328_RS02580 (U8328_02580) mtgA 548921..549649 (+) 729 WP_000047064.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  U8328_RS02585 (U8328_02585) yrbL 549646..550278 (-) 633 WP_000620396.1 PhoP regulatory network protein YrbL -
  U8328_RS02590 (U8328_02590) npr 550491..550763 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  U8328_RS02595 (U8328_02595) rapZ 550760..551614 (-) 855 WP_000243741.1 RNase adapter RapZ -
  U8328_RS02600 (U8328_02600) ptsN 551660..552151 (-) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  U8328_RS02605 (U8328_02605) hpf 552269..552556 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  U8328_RS02610 (U8328_02610) rpoN/rpoN1 552579..554012 (-) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  U8328_RS02615 (U8328_02615) lptB 554060..554785 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  U8328_RS02620 (U8328_02620) lptA 554792..555349 (-) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  U8328_RS02625 (U8328_02625) lptC 555318..555893 (-) 576 WP_000030536.1 LPS export ABC transporter periplasmic protein LptC -
  U8328_RS02630 (U8328_02630) kdsC 555890..556456 (-) 567 WP_000030006.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  U8328_RS02635 (U8328_02635) kdsD 556477..557463 (-) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  U8328_RS02640 (U8328_02640) yrbG 557477..558454 (-) 978 WP_000922873.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=837606 U8328_RS02610 WP_000809051.1 552579..554012(-) (rpoN/rpoN1) [Escherichia coli strain T-HYM1]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=837606 U8328_RS02610 WP_000809051.1 552579..554012(-) (rpoN/rpoN1) [Escherichia coli strain T-HYM1]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACACTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTGACACCGTTTT
CCGATACTGACCGCGCCATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGCCTGTTGATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGACTGATTATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACGGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGATAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCTTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCATTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465