Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   VLS60_RS02690 Genome accession   NZ_CP141749
Coordinates   455032..455709 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain 2023CK-01482     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 450032..460709
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VLS60_RS02675 (VLS60_02675) chiA 451399..454092 (+) 2694 WP_072649929.1 bifunctional chitinase/lysozyme -
  VLS60_RS02680 (VLS60_02680) bfd 454261..454455 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  VLS60_RS02685 (VLS60_02685) bfr 454527..455003 (+) 477 WP_000675504.1 bacterioferritin -
  VLS60_RS02690 (VLS60_02690) pilD 455032..455709 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  VLS60_RS02695 (VLS60_02695) gspM 455709..456170 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  VLS60_RS02700 (VLS60_02700) gspL 456167..457330 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  VLS60_RS02705 (VLS60_02705) gspK 457345..458328 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  VLS60_RS02710 (VLS60_02710) gspJ 458321..458908 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  VLS60_RS02715 (VLS60_02715) gspI 458901..459278 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  VLS60_RS02720 (VLS60_02720) gspH 459275..459784 (-) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  VLS60_RS02725 (VLS60_02725) gspG 459792..460229 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=837340 VLS60_RS02690 WP_000178161.1 455032..455709(-) (pilD) [Escherichia coli strain 2023CK-01482]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=837340 VLS60_RS02690 WP_000178161.1 455032..455709(-) (pilD) [Escherichia coli strain 2023CK-01482]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378