Detailed information NTG0837332

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: VLS60_RS01490
• Genome accession: NZ_CP141749
• Coordinates: 183677..184660 (+)
• Length: 327 a.a.
• NCBI ID: WP_001196486.1
• Uniprot ID: A7ZT85
• Organism: Escherichia coli strain 2023CK-01482
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 178677..189660

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VLS60_RS01470 (VLS60_01470)	dppA	179820..181427 (+)	1608	WP_001222883.1	dipeptide ABC transporter substrate-binding protein DppA	-
VLS60_RS01475 (VLS60_01475)	-	181461..181571 (-)	111	Protein_167	hypothetical protein	-
VLS60_RS01480 (VLS60_01480)	dppB	181735..182754 (+)	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-
VLS60_RS01485 (VLS60_01485)	dppC	182764..183666 (+)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
VLS60_RS01490 (VLS60_01490)	amiE	183677..184660 (+)	984	WP_001196486.1	dipeptide ABC transporter ATP-binding protein	Regulator
VLS60_RS01495 (VLS60_01495)	dppF	184657..185661 (+)	1005	WP_000107012.1	dipeptide ABC transporter ATP-binding subunit DppF	-
VLS60_RS01500 (VLS60_01500)	yhjV	185691..186962 (-)	1272	WP_001295225.1	aromatic amino acid transport family protein	-
VLS60_RS01505 (VLS60_01505)	ldrD	187438..187545 (+)	108	WP_000141634.1	type I toxin-antitoxin system toxic polypeptide LdrD	-
VLS60_RS01510 (VLS60_01510)	bcsG	187632..189311 (-)	1680	WP_000191622.1	cellulose biosynthesis protein BcsG	-
VLS60_RS01515 (VLS60_01515)	bcsF	189308..189499 (-)	192	WP_000988308.1	cellulose biosynthesis protein BcsF	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35844.36 Da
• Isoelectric Point: 6.5814

>NTDB_id=837332 VLS60_RS01490 WP_001196486.1 183677..184660(+) (amiE) [Escherichia coli strain 2023CK-01482]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=837332 VLS60_RS01490 WP_001196486.1 183677..184660(+) (amiE) [Escherichia coli strain 2023CK-01482]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCGCCGTTCCGCGCCGTAGACCGCATCAG
CTACAGCGTAAAACAGGGCGAAGTGGTCGGGATTGTGGGTGAGTCCGGCTCCGGTAAGTCGGTCAGTTCACTGGCGATTA
TGGGGCTGATTGATTATCCGGGCCGCGTAATGGCAGAAAAACTGGAGTTTAACGGCCAGGATTTGCAGCGTATCTCAGAA
AAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACAC
CGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCATCAGGGCGGCAACAAAAGTACCCGCCGTCAGCGAGCGATTGACC
TGCTGAATCAGGTCGGTATTCCCGATCCGGCATCGCGTCTGGATGTTTACCCGCATCAGCTTTCCGGCGGCATGAGCCAG
CGCGTGATGATCGCCATGGCGATTGCCTGTCGGCCAAAACTGCTGATTGCCGATGAACCGACCACCGCGCTGGACGTGAC
CATTCAGGCGCAAATCATCGAACTACTGCTGGAGCTACAGCAGAAAGAGAACATGGCGCTGGTGTTAATTACCCATGACC
TGGCGCTGGTGGCGGAAGCGGCACATAAAATCATCGTGATGTATGCAGGCCAGGTGGTGGAAACCGGTGATGCGCACGCC
ATCTTCCATGCGCCGCGTCACCCGTATACTCAGGCATTGCTGCGTGCGCTGCCAGAATTTGCTCAGGACAAAGAACGTCT
GGCGTCGTTGCCAGGTGTCGTTCCCGGCAAGTACGACCGCCCGAACGGCTGCCTGCTTAACCCGCGCTGCCCCTATGCCA
CTGACAGATGTCGCGCTGAAGAACCGGCGCTGAATATGCTCGCTGACGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

• ABC transporter (ABC_tran) (24-181)

3D structure

• AlphaFold DB: A7ZT85

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404