Detailed information NTG0083728

Bioinformatically predicted

Overview

• Name: chiS
• Type: Regulator
• Locus tag: VA249_RS11445
• Genome accession: NZ_AP024165
• Coordinates: 2496952..2500338 (+)
• Length: 1128 a.a.
• NCBI ID: WP_221068058.1
• Uniprot ID: -
• Organism: Vibrio alfacsensis strain 04Ya108
• Function: chitin sensor (predicted from homology) Competence regulation

Genomic Context

Location: 2491952..2505338

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VA249_RS11425 (VYA_21710)	erpA	2492234..2492575 (-)	342	WP_211916035.1	iron-sulfur cluster insertion protein ErpA	-
VA249_RS11430 (VYA_21720)	hemL	2492813..2494108 (+)	1296	WP_128810258.1	glutamate-1-semialdehyde 2,1-aminomutase	-
VA249_RS11435 (VYA_21730)	-	2494507..2495592 (+)	1086	WP_221068057.1	AI-2E family transporter	-
VA249_RS11440 (VYA_21740)	rsmC	2495700..2496725 (+)	1026	WP_211916032.1	16S rRNA (guanine(1207)-N(2))-methyltransferase RsmC	-
VA249_RS11445 (VYA_21750)	chiS	2496952..2500338 (+)	3387	WP_221068058.1	response regulator	Regulator
VA249_RS11450 (VYA_21760)	-	2501254..2502936 (+)	1683	WP_221068059.1	ABC transporter substrate-binding protein	-
VA249_RS11455 (VYA_21770)	-	2503107..2504093 (+)	987	WP_041058689.1	ABC transporter permease	-
VA249_RS11460 (VYA_21780)	-	2504096..2505121 (+)	1026	WP_221068060.1	ABC transporter permease	-

Sequence

Protein

• Length: 1128 a.a.
• Molecular weight: 127173.90 Da
• Isoelectric Point: 6.0546

>NTDB_id=83728 VA249_RS11445 WP_221068058.1 2496952..2500338(+) (chiS) [Vibrio alfacsensis strain 04Ya108]
MFKFYKKQKFKRLQSTLMTAFLVLSITPLTITAIFFLQSHTKDLEEQSTSHLLSVRDTKQQQIIDYFAAQETEVMGFVRS
ELAYASGGRFYGLVNAFSRLGQDIESARHNAQQRYIKGSGDQIKTSILPESSDYVGSERYRLLHKRYHWAYLELLKRSDF
NDILLVDIDGNVTYSINKDDNYGTNLLSGPYKDTALGKTFKRLSEDVSARRKINEDYTPVVVSDFDLENGKQVAWLGAPI
VQQGYLHSYAMFRLPNNGITKLVADINRDSSINTLLVGSNHQPRTINTKQQDIQNSLEVVDKALAGQTNVGTYKNGAGEE
FIAAFAPIQTRGITWALVVQLPEKEAFYRVHQLEKLFVIAMLIAIVLVVIASHYLSNFITSPLLKLTWAAEKVSAGDLDE
TTFNTKRKDEIGRLAISFERMQRSIREKIQTIKQQNEELESNIKLIQKQNEELQLADKLKDEFLATTSHELRTPLHGMVG
IAETLVSGANGAIPASQKYQLDIIIKSGQRLANLVDDLLDYHKMRYGSMDIQKSAVSLASATRLVLELSNHLLGNKTIRI
INQVPADLKAVSADPQRLEQVLYNLIGNAIKYTSEGKIVISANVIDEQVRVQVVDTGQGIPAEHLEHIFEPLIQAGQDTS
RYRQGAGLGLSISRQLIELMGGSLYVSSQPMVGTTFSFTLPLATEAEIEATESLAARGHFQIPEINMDSNNDLSLPENPD
GPLLYVADDEPVNLRVLESFLRLEGYRVRTVSDGPDTLTLIEQEKPELLLLDIMMPGMSGYQVCSELRKTYDHAELPIIM
LTALNQTEDRVRGFEAGANDYLSKPFNKQELAARIQAHLTASKAEMRHVENKLLESELRQRAQIEASLLETQGRLLEQLE
SAPEAIICLREDQRVRFANEAACKLFRRNLEQLRRSSADELIAPKYLTVKQPHYCGKVDIYVEDIRQNIEADILKLPEGS
GLDVMYIFNVGGGANAARIHNLETAVEVLSSYAFDGDKDQLQKLKELGGEFTRLADKALGSKKDKQELMREVLVDVMSHA
LDYWESATGETKFAFAEQSGLWRVYLDRSTLQTRTLDKYMRIETLPKTPRWRTVLSSIEFILEHCKDQSPERAHIEAQRD
KLQRLLTS

Nucleotide

• Length: 3387 bp

>NTDB_id=83728 VA249_RS11445 WP_221068058.1 2496952..2500338(+) (chiS) [Vibrio alfacsensis strain 04Ya108]
ATGTTTAAATTCTACAAAAAGCAGAAATTTAAGCGTCTACAAAGTACGCTAATGACCGCCTTTTTGGTTCTAAGTATTAC
GCCCCTGACGATCACGGCGATCTTCTTCCTGCAATCTCACACTAAAGATCTTGAAGAACAGAGCACATCGCACCTTCTTT
CAGTTCGTGATACCAAGCAGCAGCAAATCATCGATTACTTCGCAGCGCAAGAAACCGAAGTAATGGGCTTTGTGCGTTCA
GAATTAGCTTATGCCAGCGGTGGACGATTCTACGGTCTGGTTAATGCATTTAGTCGCTTGGGGCAAGATATCGAAAGCGC
AAGACACAACGCTCAACAGCGTTACATTAAAGGCAGTGGCGACCAAATCAAAACCTCGATTTTGCCTGAATCTTCAGATT
ACGTTGGCAGTGAACGCTATCGTCTGCTCCATAAGCGCTACCACTGGGCTTATCTAGAATTACTCAAGCGCTCAGATTTC
AACGATATTTTATTGGTCGACATTGATGGGAACGTCACGTATTCAATCAACAAAGACGACAACTATGGCACTAACTTACT
AAGCGGCCCATACAAAGATACCGCATTGGGTAAGACGTTTAAGCGCCTATCTGAAGATGTCAGTGCTCGCCGAAAAATCA
ATGAAGACTATACCCCCGTAGTCGTGTCTGACTTTGATTTAGAAAATGGAAAACAAGTCGCATGGCTTGGTGCACCTATT
GTACAACAAGGCTACCTACACAGTTATGCCATGTTCCGCCTACCAAATAATGGCATCACCAAGCTTGTTGCTGATATCAA
CCGTGATTCATCCATCAATACCCTTTTAGTTGGTAGCAACCATCAACCTCGAACCATTAACACCAAGCAACAAGATATTC
AAAACAGCTTGGAAGTCGTGGATAAAGCCCTTGCTGGTCAAACAAACGTTGGAACCTACAAAAATGGGGCGGGCGAAGAA
TTTATCGCCGCATTTGCGCCTATCCAAACCCGAGGAATCACTTGGGCATTGGTGGTTCAACTACCAGAAAAAGAAGCTTT
TTATCGCGTTCATCAGCTAGAAAAGCTATTCGTTATTGCAATGCTTATCGCTATTGTTCTAGTAGTCATTGCATCACATT
ACCTGTCTAACTTCATTACTTCTCCTCTACTCAAATTAACGTGGGCTGCTGAAAAGGTTTCCGCAGGTGATCTAGACGAA
ACCACATTTAATACCAAACGCAAGGATGAAATTGGCCGTCTAGCCATTAGTTTTGAGCGTATGCAACGTTCTATTCGCGA
GAAGATCCAAACCATCAAGCAACAAAACGAAGAGCTAGAGAGCAACATCAAGCTGATCCAAAAGCAAAACGAAGAGTTGC
AATTGGCAGACAAACTCAAAGATGAGTTCCTAGCAACGACGTCCCACGAATTACGTACGCCACTACACGGGATGGTAGGA
ATTGCAGAAACCCTAGTCTCGGGAGCAAATGGTGCCATTCCAGCAAGCCAAAAGTACCAACTGGATATCATCATTAAAAG
TGGTCAGCGCTTGGCAAACCTCGTAGATGATTTATTGGATTACCACAAAATGCGTTATGGCAGCATGGATATCCAAAAGT
CGGCGGTTAGCCTTGCGAGTGCCACTCGCTTGGTGCTTGAGCTTTCAAATCATTTACTTGGAAACAAAACCATCCGAATC
ATCAATCAAGTTCCAGCTGATTTAAAAGCCGTTTCAGCCGATCCTCAGCGCTTAGAGCAAGTGCTGTATAACTTGATCGG
CAATGCGATCAAGTACACCTCTGAGGGGAAAATCGTCATCTCGGCCAATGTCATTGATGAGCAAGTCCGAGTTCAAGTGG
TCGATACTGGCCAAGGTATTCCAGCTGAACACCTCGAGCATATTTTTGAACCGCTTATCCAAGCAGGACAAGATACCAGC
CGTTACCGTCAAGGTGCTGGTTTGGGGCTATCAATCAGCCGTCAATTGATTGAGTTAATGGGCGGGTCACTCTATGTGAG
CAGCCAGCCAATGGTCGGGACGACATTTAGTTTCACTCTGCCACTGGCTACGGAAGCAGAAATTGAAGCCACTGAAAGCC
TTGCTGCTCGAGGACATTTCCAAATTCCTGAAATTAACATGGACAGCAACAACGATCTTTCTCTACCAGAAAATCCTGAT
GGCCCTCTTCTTTATGTGGCCGACGATGAACCCGTTAACTTACGCGTGTTAGAAAGCTTCTTACGATTAGAAGGCTACCG
AGTACGTACCGTTTCTGATGGTCCTGATACACTAACGTTAATCGAACAGGAAAAGCCTGAACTCTTATTACTCGATATCA
TGATGCCGGGAATGAGCGGCTATCAAGTTTGTAGTGAATTGCGTAAAACATACGATCATGCCGAACTGCCAATCATTATG
CTCACCGCTCTGAATCAAACTGAAGACCGTGTTCGTGGATTTGAAGCCGGTGCCAATGATTACCTATCAAAGCCATTTAA
TAAACAAGAGCTTGCTGCTCGGATTCAGGCCCACCTAACGGCAAGTAAAGCAGAAATGCGTCATGTCGAGAATAAACTCC
TAGAGTCCGAACTCCGACAACGAGCACAGATTGAAGCAAGCTTACTAGAAACGCAAGGACGGTTACTTGAACAACTAGAA
TCCGCTCCTGAAGCCATCATTTGCTTGCGCGAAGACCAACGTGTGCGTTTTGCCAATGAAGCCGCATGTAAGCTGTTTAG
ACGAAATTTGGAGCAGCTCAGACGATCTTCGGCAGATGAGTTAATCGCGCCGAAATACCTAACAGTAAAGCAACCACATT
ACTGCGGTAAGGTCGATATATACGTTGAAGATATTCGTCAAAATATTGAAGCAGACATCCTTAAACTTCCTGAGGGTTCT
GGTCTAGACGTCATGTATATATTTAACGTCGGCGGTGGGGCAAATGCAGCGCGTATCCACAATTTGGAAACTGCCGTTGA
AGTACTGTCGAGCTATGCATTCGATGGCGACAAGGACCAACTTCAAAAATTGAAAGAGCTTGGTGGAGAATTTACTCGTC
TTGCAGATAAAGCATTGGGCAGTAAAAAGGACAAACAAGAGCTCATGCGTGAAGTACTCGTCGATGTTATGTCGCACGCC
CTTGATTATTGGGAATCCGCAACCGGAGAAACCAAATTCGCTTTTGCAGAACAAAGTGGTTTGTGGCGTGTCTATCTCGA
CCGAAGTACGCTACAAACTCGTACGCTCGATAAATATATGCGCATTGAAACGCTACCTAAAACGCCACGCTGGCGAACGG
TACTAAGCTCTATCGAATTCATTCTTGAGCATTGTAAGGACCAAAGTCCAGAGCGTGCTCATATAGAAGCCCAACGAGAC
AAGTTACAAAGATTGCTTACCAGTTAA

Domains

Predicted by InterproScan.

• HAMP domain (HAMP) (375-427)

• Response regulator receiver domain (Response_reg) (726-836)

• His Kinase A (phospho-acceptor) domain (HisKA) (460-525)

• Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c) (574-683)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
chiS	Vibrio cholerae strain A1552	76.195	100	0.763