Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   P7644_RS01640 Genome accession   NZ_CP126238
Coordinates   362223..363605 (+) Length   460 a.a.
NCBI ID   WP_404301280.1    Uniprot ID   -
Organism   Alicycliphilus denitrificans strain Ylb10     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 357223..368605
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P7644_RS01620 (P7644_01610) - 357816..358799 (-) 984 WP_404301276.1 methyltransferase domain-containing protein -
  P7644_RS01625 (P7644_01615) lplT 358860..360137 (-) 1278 WP_404301277.1 lysophospholipid transporter LplT -
  P7644_RS01630 (P7644_01620) alr 360279..361376 (+) 1098 WP_404301278.1 alanine racemase -
  P7644_RS01635 (P7644_01625) - 361456..362073 (-) 618 WP_404301279.1 glutathione S-transferase family protein -
  P7644_RS01640 (P7644_01630) radA 362223..363605 (+) 1383 WP_404301280.1 DNA repair protein RadA Machinery gene
  P7644_RS01645 (P7644_01635) - 363614..364609 (-) 996 WP_404301281.1 Bug family tripartite tricarboxylate transporter substrate binding protein -
  P7644_RS01650 (P7644_01640) - 364633..366057 (-) 1425 WP_404301282.1 amidase -
  P7644_RS01655 (P7644_01645) - 366235..366657 (+) 423 WP_404301283.1 glycerate kinase -
  P7644_RS01660 (P7644_01650) - 366701..367639 (+) 939 WP_404301284.1 branched-chain amino acid transaminase -
  P7644_RS01665 (P7644_01655) - 367647..367853 (+) 207 WP_404301285.1 zinc-finger domain-containing protein -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 47898.37 Da        Isoelectric Point: 7.4138

>NTDB_id=837223 P7644_RS01640 WP_404301280.1 362223..363605(+) (radA) [Alicycliphilus denitrificans strain Ylb10]
MAKDKTLYTCNECATTSPRWLGKCPGCGAWNTLIESVPEAGRGKNRLSGAGQYAGLAQAQAVMPLAAIEASEVARTASGI
EELDRVLGGGVVEGGVVLIGGDPGIGKSTLLLQAMDALHRVGLPTLYVTGEESGAQVALRSRRLGLEGSQVSVLAEIQLE
KILATIEATQPAVAVIDSIQTVYSDQLTSAPGSVAQVRECAAHLTRLAKATGIAVILVGHVTKEGALAGPRVLEHMVDTV
LYFEGDTHSSFRLVRAIKNRFGAVNEIGVFAMTERGLKGVANPSAIFLSQHSEPVPGSCVLVTLEGTRPLLVEIQALVDG
GGPSPRRLSVGLERDRLAMLLAVLHRHAGVACADQDVFVNAVGGVRISEPAADLAAMLAITSSLRGRALPKGFIAFGEVG
LAGEVRPAPRGQERLKEAAKLGFTMAVVPKANAPKKPIPGLEIHAVERVDEAMAAVRGLG

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=837223 P7644_RS01640 WP_404301280.1 362223..363605(+) (radA) [Alicycliphilus denitrificans strain Ylb10]
ATGGCCAAGGACAAAACCCTCTACACCTGCAACGAATGCGCCACCACCAGCCCGCGCTGGCTCGGCAAATGCCCTGGCTG
CGGCGCCTGGAACACGCTGATCGAGTCCGTGCCCGAGGCTGGCCGCGGCAAGAACCGCCTGAGTGGCGCGGGCCAGTACG
CGGGCCTGGCGCAGGCGCAGGCGGTGATGCCGCTGGCCGCCATCGAGGCCAGCGAGGTGGCGCGCACCGCCAGCGGCATC
GAGGAGCTGGACCGCGTGCTCGGCGGCGGCGTGGTGGAGGGCGGCGTGGTACTCATCGGCGGCGATCCGGGCATCGGCAA
GTCCACGCTGCTCTTGCAGGCCATGGACGCGCTGCACCGCGTGGGCCTGCCCACGCTGTACGTGACGGGCGAGGAAAGCG
GCGCCCAGGTGGCGCTGCGCTCGCGCCGGCTGGGGCTGGAGGGCAGCCAGGTGAGCGTGCTGGCCGAAATCCAGCTGGAA
AAAATCCTCGCCACCATCGAGGCCACGCAGCCCGCGGTGGCGGTGATCGACTCCATCCAGACCGTCTATTCCGACCAGCT
CACCAGTGCGCCCGGCTCGGTGGCCCAGGTGCGCGAATGCGCGGCCCACCTCACGCGCCTGGCCAAGGCCACGGGCATCG
CCGTGATCCTCGTGGGCCATGTGACCAAGGAGGGCGCGCTCGCCGGCCCGCGCGTGCTGGAGCACATGGTGGACACGGTG
CTGTACTTCGAGGGCGACACGCACAGCAGCTTCCGCCTGGTGCGTGCCATCAAGAACCGTTTCGGCGCCGTGAACGAGAT
CGGCGTGTTCGCCATGACCGAGCGCGGCCTGAAGGGCGTGGCCAACCCCAGCGCGATCTTCCTCTCCCAGCACAGCGAGC
CGGTCCCGGGTAGCTGCGTGCTGGTCACGCTGGAGGGCACGCGCCCGCTGCTGGTGGAGATCCAGGCGCTGGTCGATGGC
GGCGGCCCCAGCCCGCGGCGCCTGTCGGTAGGGCTGGAGCGCGACCGCCTGGCCATGCTGCTGGCGGTTTTGCACCGCCA
CGCGGGCGTGGCCTGCGCGGACCAGGACGTGTTCGTCAACGCCGTGGGCGGCGTGCGCATCAGCGAGCCCGCGGCCGATC
TGGCGGCGATGCTGGCCATCACCAGCAGTCTGCGTGGCCGGGCCCTGCCCAAGGGCTTCATCGCCTTCGGCGAGGTGGGC
CTGGCCGGCGAGGTGCGCCCCGCGCCGCGCGGCCAGGAGCGCCTGAAGGAGGCCGCCAAGCTGGGCTTCACCATGGCCGT
GGTGCCCAAGGCGAATGCACCCAAGAAGCCCATCCCCGGGCTGGAGATCCACGCCGTGGAGCGCGTGGATGAGGCCATGG
CCGCCGTGCGCGGCCTGGGCTGA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(68-225)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.791

98.913

0.483
  radA Streptococcus pneumoniae Rx1

46.204

100

0.463
  radA Streptococcus pneumoniae D39

46.204

100

0.463
  radA Streptococcus pneumoniae R6

46.204

100

0.463
  radA Streptococcus pneumoniae TIGR4

46.204

100

0.463
  radA Streptococcus mitis SK321

47.333

97.826

0.463
  radA Streptococcus mitis NCTC 12261

46.389

99.348

0.461