Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QZL39_RS14325 Genome accession   NZ_CP141287
Coordinates   3027541..3029265 (-) Length   574 a.a.
NCBI ID   WP_029795430.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain I14B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3022541..3034265
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QZL39_RS14290 - 3022860..3023588 (-) 729 WP_140376461.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  QZL39_RS14295 - 3023588..3024172 (-) 585 WP_308390561.1 type II secretion system protein -
  QZL39_RS14300 - 3024169..3024648 (-) 480 WP_308390560.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  QZL39_RS14305 - 3024764..3024997 (-) 234 WP_005388453.1 MSHA biogenesis protein MshA -
  QZL39_RS14310 - 3025067..3025549 (-) 483 WP_031855044.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  QZL39_RS14315 - 3025844..3026305 (-) 462 WP_024701144.1 hypothetical protein -
  QZL39_RS14320 - 3026309..3027532 (-) 1224 WP_308390559.1 type II secretion system F family protein -
  QZL39_RS14325 pilB 3027541..3029265 (-) 1725 WP_029795430.1 GspE/PulE family protein Machinery gene
  QZL39_RS14330 - 3029262..3030338 (-) 1077 WP_308390558.1 tetratricopeptide repeat protein -
  QZL39_RS14335 - 3030335..3031180 (-) 846 WP_005456312.1 AAA family ATPase -
  QZL39_RS14340 mshL 3031224..3032867 (-) 1644 WP_005456314.1 pilus (MSHA type) biogenesis protein MshL -
  QZL39_RS14345 - 3032888..3033217 (-) 330 WP_178310142.1 MSHA biogenesis protein MshK -
  QZL39_RS14350 gspM 3033210..3033860 (-) 651 WP_005497276.1 type II secretion system protein GspM -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63914.27 Da        Isoelectric Point: 5.2324

>NTDB_id=836879 QZL39_RS14325 WP_029795430.1 3027541..3029265(-) (pilB) [Vibrio parahaemolyticus strain I14B]
MKIQLRKRLGDLLVEEGIVSEDQIQQALSAQRSTGQKLGDALIDLGFITEKQMLEFLSQQLGLPLIDLGRAPVDADAVQI
LPEVHARRLRAMVVARNGDTLRVAMSDPADLFTQESLMNLLGEYNLEFIIASERQLISSFDRYYRRTKEIASFAEQLQAE
HQDVQSFDYGIDEADSEEVTVVKLVNSMFEDAVQVGASDIHIEPDDKVLRLRQRVDGVLHETILNEVNIASALVLRLKLM
AHLDISEKRLPQDGRFNIKVRGQSIDIRMSTLPTQYGESVVMRLLNQSSGLRPLEESGLPPELLVRLRRQLSRPHGMILV
TGPTGSGKTTTLYGALSELNEPGKKIITAEDPVEYRLPRITQVQINSKIDLTFSRVLRTFLRQDPDIILIGEMRDQETVE
IGLRAALTGHLVLSTLHTNDAVDSALRMIDMGAPGYLVASAVRAVVAQRLVRRVCPDCKTQDHLDESRQQWLAGRFPNQV
GVTFHKGAGCQNCNLTGYRGRIGVFEMLELEHEMMDKLRANDAVGFAQAARRSENYKPLLASAMELALQGAVSLDEVMTL
GEGDASGKTDPIFM

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=836879 QZL39_RS14325 WP_029795430.1 3027541..3029265(-) (pilB) [Vibrio parahaemolyticus strain I14B]
ATGAAAATTCAGTTGAGAAAACGCTTAGGTGACCTACTTGTTGAAGAAGGTATCGTATCGGAAGATCAAATCCAGCAAGC
TCTATCCGCTCAGCGCAGTACCGGACAAAAATTAGGTGATGCCCTGATTGACCTCGGGTTTATTACCGAAAAGCAAATGC
TGGAGTTCTTGTCTCAGCAACTTGGATTACCGTTGATCGATCTTGGACGCGCTCCAGTGGATGCGGATGCGGTGCAAATA
CTGCCAGAAGTGCATGCTCGTCGTTTACGTGCCATGGTGGTGGCTCGCAATGGTGACACTTTACGTGTTGCGATGAGTGA
TCCGGCGGATCTGTTTACCCAAGAGTCTTTAATGAACTTGCTAGGTGAGTACAACCTTGAGTTCATCATTGCCTCTGAGC
GTCAGTTGATCAGCAGTTTCGACCGTTACTACCGCCGTACTAAAGAGATCGCGTCCTTTGCTGAGCAGTTACAAGCAGAG
CACCAAGACGTACAAAGCTTTGACTATGGCATCGATGAAGCTGACAGTGAAGAAGTCACCGTTGTAAAACTGGTTAATTC
GATGTTTGAAGACGCGGTGCAAGTTGGTGCATCGGATATCCATATCGAACCAGACGATAAAGTCCTGCGTTTACGCCAGC
GTGTCGACGGTGTGCTGCATGAAACCATTCTGAACGAAGTGAACATCGCTTCTGCATTGGTATTACGCCTTAAGTTGATG
GCGCACTTGGATATTTCTGAGAAGCGTCTGCCACAAGATGGTCGCTTCAACATCAAAGTTCGTGGTCAATCTATTGATAT
TCGTATGTCGACGTTACCGACTCAGTACGGTGAATCGGTCGTAATGCGTCTGCTTAACCAGTCGTCAGGTTTACGTCCAT
TAGAAGAATCGGGTTTACCGCCTGAGCTACTGGTGCGTTTGCGTCGTCAGTTATCTCGTCCTCATGGCATGATTTTGGTA
ACCGGCCCTACGGGATCGGGTAAAACCACGACACTTTACGGTGCGTTGAGCGAGCTCAATGAACCGGGTAAAAAGATCAT
CACCGCAGAAGATCCGGTGGAATACCGTTTACCACGTATCACGCAAGTACAGATCAACAGCAAGATCGACTTAACGTTTT
CACGCGTGCTACGCACCTTCTTGCGTCAAGACCCAGACATCATTTTGATTGGTGAGATGCGTGACCAAGAGACGGTAGAG
ATTGGCTTGCGAGCAGCATTAACTGGTCACTTAGTATTGAGTACGTTGCATACCAACGATGCGGTCGACAGCGCACTGCG
TATGATCGACATGGGGGCGCCAGGCTATCTCGTTGCCAGTGCCGTGCGTGCCGTTGTAGCTCAGCGATTGGTTCGCCGTG
TTTGTCCAGATTGTAAAACCCAAGATCATCTCGATGAGTCGCGTCAACAATGGTTAGCGGGGCGTTTTCCAAATCAAGTT
GGGGTGACTTTCCACAAAGGCGCAGGTTGTCAAAACTGTAACTTGACGGGGTATCGAGGTCGAATCGGTGTGTTTGAAAT
GCTTGAGCTGGAACATGAGATGATGGACAAGCTGCGTGCGAATGATGCGGTTGGCTTTGCACAAGCGGCTCGACGTTCAG
AAAACTACAAACCGCTTCTTGCCTCAGCAATGGAGCTTGCACTGCAAGGTGCTGTGAGCTTGGATGAAGTGATGACCCTC
GGCGAGGGTGACGCTTCGGGTAAAACTGATCCCATTTTCATGTAG

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(187-453)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.64

97.387

0.376
  pilB/pilB1 Synechocystis sp. PCC 6803

34.272

100

0.361