Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   OO975_RS01940 Genome accession   NZ_AP026961
Coordinates   421251..421928 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain EC20-72C-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 416251..426928
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OO975_RS01925 (EC2072C1_03840) chiA 417618..420311 (+) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -
  OO975_RS01930 (EC2072C1_03850) bfd 420480..420674 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  OO975_RS01935 (EC2072C1_03860) bfr 420746..421222 (+) 477 WP_000675504.1 bacterioferritin -
  OO975_RS01940 (EC2072C1_03870) pilD 421251..421928 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  OO975_RS01945 (EC2072C1_03880) gspM 421928..422389 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  OO975_RS01950 gspL 422386..423548 (-) 1163 Protein_387 type II secretion system protein GspL -
  OO975_RS01955 (EC2072C1_03910) gspK 423563..424546 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  OO975_RS01960 (EC2072C1_03920) gspJ 424539..425126 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  OO975_RS01965 (EC2072C1_03930) gspI 425119..425496 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  OO975_RS01970 (EC2072C1_03940) gspH 425493..426002 (-) 510 WP_024238847.1 type II secretion system minor pseudopilin GspH -
  OO975_RS01975 (EC2072C1_03950) gspG 426010..426447 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=83676 OO975_RS01940 WP_000178161.1 421251..421928(-) (pilD) [Escherichia coli strain EC20-72C-1]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=83676 OO975_RS01940 WP_000178161.1 421251..421928(-) (pilD) [Escherichia coli strain EC20-72C-1]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378

Multiple sequence alignment