Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   OO845_RS16110 Genome accession   NZ_AP026937
Coordinates   3352910..3353377 (-) Length   155 a.a.
NCBI ID   WP_000340171.1    Uniprot ID   -
Organism   Escherichia coli strain EC20-4B-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3347910..3358377
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OO845_RS16085 (EC204B2_30620) rpsG 3348051..3348521 (+) 471 WP_001138043.1 30S ribosomal protein S7 -
  OO845_RS16090 (EC204B2_30630) fusA 3348618..3350732 (+) 2115 WP_000124700.1 elongation factor G -
  OO845_RS16095 (EC204B2_30640) tuf 3350803..3351987 (+) 1185 WP_000031783.1 elongation factor Tu -
  OO845_RS16100 (EC204B2_30650) bfd 3352170..3352364 (+) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  OO845_RS16105 (EC204B2_30660) bfr 3352437..3352913 (+) 477 WP_000675515.1 bacterioferritin -
  OO845_RS16110 (EC204B2_30670) pilD 3352910..3353377 (-) 468 WP_000340171.1 prepilin peptidase Machinery gene
  OO845_RS16115 (EC204B2_30680) rpsJ 3353756..3354067 (+) 312 WP_001181004.1 30S ribosomal protein S10 -
  OO845_RS16120 (EC204B2_30690) rplC 3354100..3354729 (+) 630 WP_000579833.1 50S ribosomal protein L3 -
  OO845_RS16125 (EC204B2_30700) rplD 3354740..3355345 (+) 606 WP_000424395.1 50S ribosomal protein L4 -
  OO845_RS16130 (EC204B2_30710) rplW 3355342..3355644 (+) 303 WP_000617544.1 50S ribosomal protein L23 -
  OO845_RS16135 (EC204B2_30720) rplB 3355662..3356483 (+) 822 WP_000301864.1 50S ribosomal protein L2 -
  OO845_RS16140 (EC204B2_30730) rpsS 3356500..3356778 (+) 279 WP_001138117.1 30S ribosomal protein S19 -
  OO845_RS16145 (EC204B2_30740) rplV 3356793..3357125 (+) 333 WP_000447529.1 50S ribosomal protein L22 -
  OO845_RS16150 (EC204B2_30750) rpsC 3357143..3357844 (+) 702 WP_000529945.1 30S ribosomal protein S3 -
  OO845_RS16155 (EC204B2_30760) rplP 3357857..3358267 (+) 411 WP_000941212.1 50S ribosomal protein L16 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 16938.08 Da        Isoelectric Point: 8.1296

>NTDB_id=83645 OO845_RS16110 WP_000340171.1 3352910..3353377(-) (pilD) [Escherichia coli strain EC20-4B-2]
MDATLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFSQVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=83645 OO845_RS16110 WP_000340171.1 3352910..3353377(-) (pilD) [Escherichia coli strain EC20-4B-2]
ATGGACGCTACCCTTCCCTTTCTGATTTTGTACGCTTGTTTATCTGTCCTACTTTTTTTATGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACCTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTCTCAGGTTTGTAATCCTGACGGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGTCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACCTGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTGTTGGTCTGCTCATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTGTAGGTTGGGATAGTTTGCTGGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(10-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.8

80.645

0.361
  pilD Neisseria gonorrhoeae MS11

38.095

94.839

0.361

Multiple sequence alignment