Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   U8P27_RS06090 Genome accession   NZ_CP141093
Coordinates   1280967..1282400 (-) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli strain KE41     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1275967..1287400
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U8P27_RS06055 (U8P27_06055) elbB 1276658..1277311 (+) 654 WP_000719791.1 isoprenoid biosynthesis glyoxalase ElbB -
  U8P27_RS06060 (U8P27_06060) mtgA 1277308..1278036 (+) 729 WP_000047069.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  U8P27_RS06065 (U8P27_06065) yrbL 1278033..1278665 (-) 633 WP_001539838.1 PhoP regulatory network protein YrbL -
  U8P27_RS06070 (U8P27_06070) npr 1278879..1279151 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  U8P27_RS06075 (U8P27_06075) rapZ 1279148..1280002 (-) 855 WP_000243741.1 RNase adapter RapZ -
  U8P27_RS06080 (U8P27_06080) ptsN 1280048..1280539 (-) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  U8P27_RS06085 (U8P27_06085) hpf 1280657..1280944 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  U8P27_RS06090 (U8P27_06090) rpoN/rpoN1 1280967..1282400 (-) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  U8P27_RS06095 (U8P27_06095) lptB 1282448..1283173 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  U8P27_RS06100 (U8P27_06100) lptA 1283180..1283737 (-) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  U8P27_RS06105 (U8P27_06105) lptC 1283706..1284281 (-) 576 WP_001539833.1 LPS export ABC transporter periplasmic protein LptC -
  U8P27_RS06110 (U8P27_06110) kdsC 1284278..1284844 (-) 567 WP_000030015.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  U8P27_RS06115 (U8P27_06115) kdsD 1284865..1285851 (-) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  U8P27_RS06120 (U8P27_06120) yrbG 1285865..1286842 (-) 978 WP_000922880.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=836150 U8P27_RS06090 WP_000809051.1 1280967..1282400(-) (rpoN/rpoN1) [Escherichia coli strain KE41]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=836150 U8P27_RS06090 WP_000809051.1 1280967..1282400(-) (rpoN/rpoN1) [Escherichia coli strain KE41]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGATACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACACTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTATCAGGGCGAAACGACACAGACCTTACAGGATTACCTGATGTGGCAGGTCGAGTTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATTGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAAAGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465