Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   U8P27_RS05430 Genome accession   NZ_CP141093
Coordinates   1162767..1163444 (-) Length   225 a.a.
NCBI ID   WP_000178175.1    Uniprot ID   A0A0H2Z3H2
Organism   Escherichia coli strain KE41     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1157767..1168444
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U8P27_RS05415 (U8P27_05415) chiA 1159125..1161827 (+) 2703 WP_000773146.1 bifunctional chitinase/lysozyme -
  U8P27_RS05420 (U8P27_05420) bfd 1161996..1162190 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  U8P27_RS05425 (U8P27_05425) bfr 1162262..1162738 (+) 477 WP_000675504.1 bacterioferritin -
  U8P27_RS05430 (U8P27_05430) pilD 1162767..1163444 (-) 678 WP_000178175.1 prepilin peptidase GspO Machinery gene
  U8P27_RS05435 (U8P27_05435) gspM 1163444..1163905 (-) 462 WP_001298207.1 type II secretion system protein GspM -
  U8P27_RS05440 (U8P27_05440) gspL 1163902..1165065 (-) 1164 Protein_1050 type II secretion system protein GspL -
  U8P27_RS05445 (U8P27_05445) gspK 1165080..1166063 (-) 984 WP_001058513.1 type II secretion system minor pseudopilin GspK -
  U8P27_RS05450 (U8P27_05450) gspJ 1166056..1166643 (-) 588 WP_000610632.1 type II secretion system minor pseudopilin GspJ -
  U8P27_RS05455 (U8P27_05455) gspI 1166636..1167013 (-) 378 WP_001041748.1 type II secretion system minor pseudopilin GspI -
  U8P27_RS05460 (U8P27_05460) gspH 1167010..1167519 (-) 510 WP_001539909.1 type II secretion system minor pseudopilin GspH -
  U8P27_RS05465 (U8P27_05465) gspG 1167527..1167964 (-) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24870.75 Da        Isoelectric Point: 7.6364

>NTDB_id=836147 U8P27_RS05430 WP_000178175.1 1162767..1163444(-) (pilD) [Escherichia coli strain KE41]
MTMLLPLFILVGFIAGYFVNVMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=836147 U8P27_RS05430 WP_000178175.1 1162767..1163444(-) (pilD) [Escherichia coli strain KE41]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGTCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATTGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2Z3H2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.387
  pilD Vibrio cholerae strain A1552

34.661

100

0.387