Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   U8P30_RS11140 Genome accession   NZ_CP141084
Coordinates   2346861..2348294 (+) Length   477 a.a.
NCBI ID   WP_062893489.1    Uniprot ID   -
Organism   Escherichia coli strain KE48     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2341861..2353294
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U8P30_RS11110 (U8P30_11110) yrbG 2342419..2343396 (+) 978 WP_000922880.1 calcium/sodium antiporter -
  U8P30_RS11115 (U8P30_11115) kdsD 2343410..2344396 (+) 987 WP_001305135.1 arabinose-5-phosphate isomerase KdsD -
  U8P30_RS11120 (U8P30_11120) kdsC 2344417..2344983 (+) 567 WP_322882294.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  U8P30_RS11125 (U8P30_11125) lptC 2344980..2345555 (+) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  U8P30_RS11130 (U8P30_11130) lptA 2345524..2346081 (+) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  U8P30_RS11135 (U8P30_11135) lptB 2346088..2346813 (+) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  U8P30_RS11140 (U8P30_11140) rpoN/rpoN1 2346861..2348294 (+) 1434 WP_062893489.1 RNA polymerase factor sigma-54 Machinery gene
  U8P30_RS11145 (U8P30_11145) hpf 2348317..2348604 (+) 288 WP_001176599.1 ribosome hibernation promoting factor -
  U8P30_RS11150 (U8P30_11150) ptsN 2348722..2349213 (+) 492 WP_000183674.1 PTS IIA-like nitrogen regulatory protein PtsN -
  U8P30_RS11155 (U8P30_11155) rapZ 2349259..2350113 (+) 855 WP_000243741.1 RNase adapter RapZ -
  U8P30_RS11160 (U8P30_11160) npr 2350110..2350382 (+) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  U8P30_RS11165 (U8P30_11165) yrbL 2350595..2351227 (+) 633 WP_000620393.1 PhoP regulatory network protein YrbL -
  U8P30_RS11170 (U8P30_11170) mtgA 2351224..2351952 (-) 729 WP_000047069.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  U8P30_RS11175 (U8P30_11175) elbB 2351949..2352602 (-) 654 WP_000719791.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54023.82 Da        Isoelectric Point: 4.3639

>NTDB_id=836066 U8P30_RS11140 WP_062893489.1 2346861..2348294(+) (rpoN/rpoN1) [Escherichia coli strain KE48]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLITAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=836066 U8P30_RS11140 WP_062893489.1 2346861..2348294(+) (rpoN/rpoN1) [Escherichia coli strain KE48]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGCAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACACTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTATCAGGGCGAAACGACACAGACCTTACAGGATTACCTGATGTGGCAGGTCGAGTTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATTGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGCCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCATGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTATTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGATAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGCGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCCG
TCGAGATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCACGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CTCGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465