Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   QNH44_RS18860 Genome accession   NZ_CP126102
Coordinates   3701726..3702796 (-) Length   356 a.a.
NCBI ID   WP_163143398.1    Uniprot ID   -
Organism   Cytobacillus firmus strain LN5     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3696726..3707796
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QNH44_RS18815 (QNH44_18815) - 3697218..3698012 (+) 795 WP_048008256.1 YqhG family protein -
  QNH44_RS18820 (QNH44_18820) - 3698058..3698243 (-) 186 WP_095245709.1 YqzE family protein -
  QNH44_RS18825 (QNH44_18825) - 3698322..3698819 (-) 498 WP_227888232.1 shikimate kinase -
  QNH44_RS18830 (QNH44_18830) comGG 3698832..3699215 (-) 384 WP_163143381.1 competence type IV pilus minor pilin ComGG -
  QNH44_RS18835 (QNH44_18835) comGF 3699205..3699684 (-) 480 WP_349632665.1 competence type IV pilus minor pilin ComGF -
  QNH44_RS18840 (QNH44_18840) - 3699635..3699964 (-) 330 WP_227888230.1 phosphatase -
  QNH44_RS18845 (QNH44_18845) comGD 3699948..3700388 (-) 441 WP_174749600.1 competence type IV pilus minor pilin ComGD -
  QNH44_RS18850 (QNH44_18850) comGC 3700385..3700708 (-) 324 WP_163143392.1 competence type IV pilus major pilin ComGC -
  QNH44_RS18855 (QNH44_18855) comGB 3700708..3701739 (-) 1032 WP_163143395.1 competence type IV pilus assembly protein ComGB -
  QNH44_RS18860 (QNH44_18860) comGA 3701726..3702796 (-) 1071 WP_163143398.1 competence type IV pilus ATPase ComGA Machinery gene
  QNH44_RS18870 (QNH44_18870) - 3703065..3703418 (-) 354 WP_048008265.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  QNH44_RS18875 (QNH44_18875) - 3703665..3704363 (+) 699 WP_163143401.1 helix-turn-helix domain-containing protein -
  QNH44_RS18880 (QNH44_18880) - 3704516..3704761 (+) 246 WP_009332938.1 DUF2626 domain-containing protein -
  QNH44_RS18885 (QNH44_18885) - 3704796..3705866 (-) 1071 WP_283914527.1 SAM-dependent methyltransferase -
  QNH44_RS18890 (QNH44_18890) - 3706071..3706706 (-) 636 WP_163143404.1 MBL fold metallo-hydrolase -
  QNH44_RS18895 (QNH44_18895) - 3706794..3707273 (+) 480 WP_163143407.1 hypothetical protein -
  QNH44_RS18900 (QNH44_18900) - 3707399..3707572 (+) 174 WP_048008269.1 DUF2759 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40094.25 Da        Isoelectric Point: 8.0777

>NTDB_id=836002 QNH44_RS18860 WP_163143398.1 3701726..3702796(-) (comGA) [Cytobacillus firmus strain LN5]
MSIIERLAERIIKDAVRSHASDIHIIPRRKDTLIQLRFGSQLTPRLYLPKEECDRLISHFKFTASMDIGEKRRPQSGAYS
LEVDGQMIGLRFSTLPSSHSESLVIRILPQQEQIPFYQISLFPDMTRKMLALLKHAHGLIIFTGPTGSGKTTTLYSLLNE
TSHMFHRNVITLEDPIEKENDLVLQVQVNEKAGVSYSAGLKAILRHDPDIIMVGEIRDAETAKIAVRAALTGHLVLSTMH
TRDAEGAVYRLAEFGVNVLEIEQTLVAVTAQRLVELNCPYCEKECSPFCYGYGRWKRASVFELLAGRALNASIKNARGEN
SLVKYRTLKEVITKGIALGYIKETEYSRWVHDDGEA

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=836002 QNH44_RS18860 WP_163143398.1 3701726..3702796(-) (comGA) [Cytobacillus firmus strain LN5]
TTGAGTATAATTGAAAGATTGGCAGAACGAATTATTAAAGACGCGGTCAGAAGCCATGCTTCCGACATCCACATCATTCC
CCGAAGAAAAGACACACTCATTCAGCTGAGGTTCGGCAGCCAGTTGACTCCAAGGCTTTATCTTCCCAAAGAAGAGTGTG
ACAGATTGATTTCCCATTTTAAATTTACAGCATCAATGGATATTGGTGAAAAAAGAAGGCCGCAGAGCGGTGCATACTCA
CTTGAAGTAGACGGACAAATGATTGGCCTGCGCTTTTCAACCCTCCCATCAAGCCACAGCGAGAGCCTCGTTATCAGAAT
TCTTCCCCAGCAGGAACAAATTCCTTTTTACCAAATCAGCCTTTTTCCAGACATGACAAGAAAAATGCTGGCACTGCTGA
AGCATGCTCATGGTTTAATTATTTTCACTGGCCCGACCGGCAGCGGTAAAACCACAACTTTGTATTCCCTTCTGAATGAA
ACCTCCCATATGTTCCATCGCAATGTCATCACCCTGGAAGATCCCATTGAAAAAGAGAATGACTTAGTTCTTCAAGTCCA
AGTGAATGAGAAAGCTGGCGTGTCTTATTCTGCAGGCTTAAAAGCAATCCTTCGCCATGACCCGGATATCATTATGGTCG
GTGAAATCCGGGATGCGGAAACGGCGAAGATTGCAGTTAGAGCAGCTCTCACCGGTCACCTTGTTTTAAGCACCATGCAT
ACACGGGATGCCGAGGGGGCTGTATACCGTTTAGCTGAGTTTGGAGTGAACGTGCTGGAAATTGAGCAGACTTTGGTGGC
GGTCACCGCACAGCGCCTCGTCGAATTGAATTGTCCATATTGTGAAAAGGAATGCTCCCCATTCTGCTATGGATATGGCA
GGTGGAAAAGGGCGAGTGTGTTTGAGCTGCTTGCAGGAAGGGCGCTCAATGCCTCCATAAAGAATGCAAGAGGGGAAAAC
AGCCTTGTTAAGTATCGAACACTGAAGGAAGTCATAACTAAAGGGATTGCTCTGGGCTATATAAAGGAAACGGAGTACAG
CAGGTGGGTGCATGACGATGGAGAAGCATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.337

100

0.553
  pilB Glaesserella parasuis strain SC1401

39.714

98.315

0.39