Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   U8P20_RS03540 Genome accession   NZ_CP141080
Coordinates   760912..761589 (-) Length   225 a.a.
NCBI ID   WP_000178174.1    Uniprot ID   A0A0H2VBG7
Organism   Escherichia coli strain KE51     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 755912..766589
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U8P20_RS03525 (U8P20_03525) chiA 757270..759972 (+) 2703 WP_000773147.1 bifunctional chitinase/lysozyme -
  U8P20_RS03530 (U8P20_03530) bfd 760141..760335 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  U8P20_RS03535 (U8P20_03535) bfr 760407..760883 (+) 477 WP_000675504.1 bacterioferritin -
  U8P20_RS03540 (U8P20_03540) pilD 760912..761589 (-) 678 WP_000178174.1 prepilin peptidase GspO Machinery gene
  U8P20_RS03545 (U8P20_03545) gspM 761589..762050 (-) 462 WP_001298207.1 type II secretion system protein GspM -
  U8P20_RS03550 (U8P20_03550) gspL 762047..763210 (-) 1164 WP_001304915.1 type II secretion system protein GspL -
  U8P20_RS03555 (U8P20_03555) gspK 763225..764208 (-) 984 Protein_687 type II secretion system minor pseudopilin GspK -
  U8P20_RS03560 (U8P20_03560) gspJ 764201..764788 (-) 588 WP_000610637.1 type II secretion system minor pseudopilin GspJ -
  U8P20_RS03565 (U8P20_03565) gspI 764781..765158 (-) 378 WP_001041742.1 type II secretion system minor pseudopilin GspI -
  U8P20_RS03570 (U8P20_03570) gspH 765155..765664 (-) 510 WP_001076033.1 type II secretion system minor pseudopilin GspH -
  U8P20_RS03575 (U8P20_03575) gspG 765672..766109 (-) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24866.76 Da        Isoelectric Point: 7.6364

>NTDB_id=835875 U8P20_RS03540 WP_000178174.1 760912..761589(-) (pilD) [Escherichia coli strain KE51]
MTMLLPLFILVGFIAGYFVNVMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTIPTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=835875 U8P20_RS03540 WP_000178174.1 760912..761589(-) (pilD) [Escherichia coli strain KE51]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGTCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATTGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTACCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATACCCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VBG7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.387
  pilD Vibrio cholerae strain A1552

34.661

100

0.387