Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   QNK06_RS13770 Genome accession   NZ_CP126097
Coordinates   2609035..2609445 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis strain KF24     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2604035..2614445
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QNK06_RS13740 (QNK06_13740) - 2604121..2604261 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  QNK06_RS13745 (QNK06_13745) yqeF 2604567..2605298 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  QNK06_RS13750 (QNK06_13750) cwlH 2605550..2606302 (-) 753 WP_072174288.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  QNK06_RS13755 (QNK06_13755) yqeD 2606489..2607115 (+) 627 WP_161619430.1 TVP38/TMEM64 family protein -
  QNK06_RS13760 (QNK06_13760) gnd 2607135..2608028 (-) 894 WP_070547557.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  QNK06_RS13765 (QNK06_13765) yqeB 2608280..2609002 (+) 723 WP_049141193.1 hypothetical protein -
  QNK06_RS13770 (QNK06_13770) nucA/comI 2609035..2609445 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  QNK06_RS13775 (QNK06_13775) - 2609641..2609988 (+) 348 Protein_2668 sigma-70 family RNA polymerase sigma factor -
  QNK06_RS13780 (QNK06_13780) spoIVCA 2610019..2611479 (-) 1461 WP_283934478.1 site-specific DNA recombinase SpoIVCA -
  QNK06_RS13785 (QNK06_13785) - 2611437..2611615 (-) 179 Protein_2670 hypothetical protein -
  QNK06_RS13790 (QNK06_13790) - 2612048..2612641 (-) 594 WP_029318098.1 TetR/AcrR family transcriptional regulator -
  QNK06_RS13795 (QNK06_13795) - 2612799..2613794 (+) 996 WP_029318099.1 acryloyl-CoA reductase -
  QNK06_RS13800 (QNK06_13800) - 2614122..2614355 (+) 234 Protein_2673 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=835728 QNK06_RS13770 WP_009967785.1 2609035..2609445(-) (nucA/comI) [Bacillus subtilis strain KF24]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=835728 QNK06_RS13770 WP_009967785.1 2609035..2609445(-) (nucA/comI) [Bacillus subtilis strain KF24]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAGCAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACTGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529