Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   QNH21_RS05245 Genome accession   NZ_CP126090
Coordinates   1076256..1079960 (+) Length   1234 a.a.
NCBI ID   WP_283859994.1    Uniprot ID   -
Organism   Bacillus safensis strain CEW AP102     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1071256..1084960
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QNH21_RS05230 (QNH21_05230) - 1071563..1071973 (+) 411 WP_024425497.1 DoxX family protein -
  QNH21_RS05235 (QNH21_05235) - 1072170..1072616 (-) 447 WP_024425498.1 ferritin-like domain-containing protein -
  QNH21_RS05240 (QNH21_05240) addB 1072769..1076278 (+) 3510 WP_283859993.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  QNH21_RS05245 (QNH21_05245) addA 1076256..1079960 (+) 3705 WP_283859994.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  QNH21_RS05250 (QNH21_05250) sbcD 1079987..1081162 (+) 1176 WP_283859995.1 exonuclease subunit SbcD -
  QNH21_RS05255 (QNH21_05255) - 1081159..1084554 (+) 3396 WP_283859996.1 SMC family ATPase -
  QNH21_RS05260 (QNH21_05260) - 1084575..1084874 (+) 300 WP_024425503.1 hypothetical protein -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 142255.31 Da        Isoelectric Point: 5.0351

>NTDB_id=835279 QNH21_RS05245 WP_283859994.1 1076256..1079960(+) (addA) [Bacillus safensis strain CEW AP102]
MQIPKPNNSTWTDDQWEAIVSEGQDILVAAAAGSGKTAVLVERLIRKMTRPEDPVDVDRLLVVTFTNASAAEMKHRITEA
LEKELAKNPGSLHMRRQLSLMNRANISTLHSFCLQVLRTFYYEIDLDPGFRLADQTEGELLGDEVLDELFEDEYKAGKPA
FFELVDRYTSDRHDLDLQWLVKRIYEFSRSHPSPEQWMRAFLSLYDVEAQTKVEQLPFYPYIKEDLSLVFRSCLELLERA
LTLSKEPGGPAPRAENFIDDLEQVNELIRHQDDFEKLYELLPNINFKRLKTCKGDEYDPVLLEKATDARNQAKKQLEKLK
DEYFMRSPAQHLKSLAEMKPVVETLVELVIQFGQRFERAKQEKSIVDFSDLEHYCLRILAEQDAEGNLIETEAAKYYQQQ
FEEVLVDEYQDTNLVQETILKLVSKGEHAAEGNLFMVGDVKQSIYRFRLAEPMLFLTKYKQFTPDGKETGKRIDLNKNFR
SRSDVLDSTNFLFKQLMGETVGEIEYDEQAELKLGASYPESEDTTTEMLLVHLDQQEAETDEEREELETVQLEARVIAKK
IRQLVEQPFQVYDSKQQMTRNLQYRDIVILLRSMPWAPQMMEELKKQGIPVYANLSSGYFEATEVSVILSLLKVIDNPYQ
DIPLAAVLRSPIVHIDENEMALIRTSDKKGTYYDAVKAFMSGTHSDHPTCKKLERFFRLLRKWRDFSMNHSVAELIWEVY
RDTQYLDYVGGMPGGKQRQANLRALYDRAKQYEKAAFRGLFRFLRFIERMQERGDDLGAAKTFSETEDVVRMMTIHSSKG
LEFPVVFTAGLGRNFNMMDLNQSYLLDKELGFGSKYIHPELRISYATLPLVAMKKKMRKELLSEELRVLYVALTRAKEKL
FLVGSVKNQVKALSKWQNAATGEDWLIPDFERYQSKTYLDFIGPALIRHQAMSSILEEKGDVVLSHPSAFTISFTQASDL
LKENMSLEKKQQDEVVQALMDGLPVEGYGDADEKVAERLSWRYPYLAASQVGTKQSVSEIKRMKEIQDEYSVPSSLRRAH
ATLYDRPAFMKKKALTAAEQGTAMHTVMQHIPLSSDEPLDEIRIHQLLDSLQQRDLLTNEQVQSIDQEGISAFFSTSIGQ
KLLKADWVKREVSFSMALPVKEVYSHIDTEGEPVLIQGMIDCLFETDGKLYLLDYKTDRVQGRYTGGLEAAVPILKKRYE
TQIALYAQAVERLTNRTLEEKILYFFDGNLEISL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=835279 QNH21_RS05245 WP_283859994.1 1076256..1079960(+) (addA) [Bacillus safensis strain CEW AP102]
ATGCAAATACCAAAACCGAATAACAGTACTTGGACGGATGACCAGTGGGAAGCCATCGTTTCAGAAGGACAAGATATCCT
TGTTGCGGCGGCGGCAGGGTCAGGTAAAACAGCTGTTTTGGTTGAACGGCTGATTCGAAAAATGACCCGTCCTGAAGATC
CAGTTGATGTGGATCGTCTGCTCGTTGTGACTTTCACAAATGCATCTGCGGCAGAGATGAAGCATCGGATCACAGAAGCA
CTTGAAAAGGAATTAGCCAAAAACCCCGGGTCCCTTCATATGAGAAGACAGCTGTCGCTCATGAATCGTGCCAATATTTC
CACCCTGCACTCCTTTTGCTTGCAGGTTTTGCGCACCTTTTACTATGAGATTGACCTAGATCCAGGCTTTCGTTTGGCTG
ATCAAACAGAAGGAGAATTATTAGGAGATGAAGTGTTAGATGAACTGTTTGAGGATGAATATAAGGCTGGAAAGCCAGCG
TTCTTTGAACTGGTTGATCGTTACACTTCTGATCGTCATGATTTAGACTTGCAATGGCTTGTGAAGAGAATTTATGAGTT
TTCCAGATCTCACCCTTCACCTGAGCAATGGATGAGGGCATTCCTTTCTCTTTATGATGTAGAAGCTCAAACAAAAGTAG
AACAATTACCGTTTTACCCATATATCAAAGAGGACCTTTCTCTCGTTTTTCGAAGCTGTCTGGAACTGCTCGAACGAGCC
CTCACCCTATCAAAAGAACCGGGCGGTCCGGCACCGAGAGCAGAAAATTTTATAGATGATTTAGAGCAAGTCAATGAATT
AATCAGGCACCAAGATGATTTTGAGAAGCTTTACGAGCTTTTGCCAAACATCAATTTTAAAAGGCTCAAAACATGTAAAG
GGGACGAATATGACCCTGTGCTGTTAGAAAAAGCCACAGATGCACGCAATCAAGCAAAAAAACAATTAGAAAAGCTTAAA
GATGAATACTTTATGCGCAGTCCCGCCCAGCATTTGAAAAGCTTAGCTGAAATGAAGCCGGTGGTCGAGACACTTGTGGA
GCTTGTCATCCAATTTGGACAGCGTTTCGAAAGAGCGAAGCAAGAAAAATCAATCGTGGATTTTTCGGATTTAGAGCATT
ATTGCTTACGCATTTTAGCAGAGCAGGACGCGGAAGGAAATTTGATCGAAACAGAAGCGGCCAAGTACTATCAACAGCAG
TTTGAAGAAGTGCTCGTTGATGAATATCAAGATACAAACCTTGTGCAAGAAACGATTTTAAAGCTTGTATCTAAAGGGGA
ACATGCTGCAGAGGGCAACCTGTTTATGGTTGGTGATGTAAAGCAGTCCATATATCGTTTTAGACTGGCTGAGCCCATGC
TCTTTTTAACCAAATATAAACAATTCACACCAGACGGCAAAGAAACAGGGAAGAGAATTGATTTAAATAAAAACTTCCGC
AGCCGTTCAGATGTGTTAGACAGTACGAACTTTTTGTTCAAACAGCTGATGGGGGAAACAGTTGGAGAAATTGAATATGA
TGAACAAGCTGAATTAAAGCTTGGAGCGAGTTATCCAGAAAGCGAAGATACAACGACAGAAATGCTTCTTGTACATTTAG
ATCAACAGGAAGCAGAAACTGACGAAGAGCGAGAAGAGCTAGAAACCGTACAGCTTGAAGCGAGAGTCATTGCTAAAAAA
ATAAGACAATTGGTGGAGCAGCCATTTCAAGTGTATGATTCAAAACAGCAAATGACCCGTAACTTGCAGTATCGGGACAT
TGTGATTTTGCTTCGCTCCATGCCGTGGGCACCGCAAATGATGGAGGAATTGAAGAAGCAGGGAATCCCTGTGTATGCGA
ATCTTTCCTCTGGTTATTTTGAAGCAACAGAGGTGTCTGTGATTCTTTCTCTGTTAAAAGTCATTGATAACCCATATCAA
GATATCCCGCTTGCAGCTGTATTAAGATCACCAATTGTTCATATAGACGAAAATGAAATGGCACTGATCCGCACGAGTGA
TAAAAAAGGCACCTATTATGATGCTGTGAAAGCATTTATGAGTGGGACTCATTCAGATCACCCTACATGCAAAAAACTAG
AACGATTTTTCAGGTTGTTGCGTAAGTGGCGTGATTTCTCTATGAATCATTCAGTAGCGGAATTGATTTGGGAAGTATAC
CGCGATACGCAGTATCTAGATTACGTCGGCGGGATGCCTGGAGGCAAACAGAGACAGGCGAATCTCCGTGCTCTTTATGA
TCGAGCGAAGCAGTATGAAAAAGCTGCTTTTAGAGGGCTGTTCCGTTTTCTTCGCTTTATCGAAAGAATGCAGGAGCGTG
GAGATGATCTTGGGGCGGCAAAAACGTTCAGTGAAACAGAGGACGTTGTTCGCATGATGACGATCCACAGCAGTAAAGGA
TTAGAATTTCCAGTCGTCTTTACCGCAGGACTCGGCAGAAACTTTAATATGATGGACTTAAACCAGTCCTACTTGCTTGA
TAAAGAATTAGGCTTTGGAAGCAAATATATTCATCCAGAGCTGAGAATTAGTTATGCAACATTACCGCTTGTTGCGATGA
AAAAAAAGATGAGGAAAGAGCTATTATCTGAAGAGCTGAGAGTTCTATATGTAGCGCTCACAAGGGCTAAGGAAAAATTA
TTTCTTGTCGGCTCTGTCAAAAATCAGGTGAAGGCATTAAGCAAATGGCAAAATGCTGCAACCGGAGAGGATTGGCTCAT
CCCTGATTTTGAACGTTATCAATCGAAAACATACTTAGACTTTATTGGTCCTGCTCTCATCCGTCACCAAGCAATGTCAT
CTATTTTAGAAGAAAAAGGTGATGTTGTTCTTTCGCACCCATCAGCATTCACAATCTCATTTACTCAAGCTTCCGATCTT
CTAAAAGAAAATATGTCACTTGAAAAGAAGCAGCAAGATGAGGTGGTACAAGCATTGATGGACGGCTTACCTGTTGAAGG
TTATGGTGATGCTGATGAGAAAGTAGCAGAGAGACTTTCATGGAGATACCCGTATCTAGCGGCCTCACAAGTCGGCACAA
AGCAGTCTGTTTCAGAGATAAAGAGAATGAAAGAAATACAGGATGAGTACAGTGTTCCTTCTTCTCTTAGAAGAGCTCAT
GCAACGTTGTATGACCGGCCGGCTTTCATGAAGAAAAAAGCACTCACTGCCGCAGAGCAAGGCACCGCAATGCATACAGT
CATGCAGCATATTCCGCTTTCTTCTGACGAGCCCCTCGATGAAATCCGTATACATCAGCTGCTCGATTCCTTACAGCAGC
GAGACTTGCTAACAAATGAGCAAGTTCAGTCAATTGATCAAGAAGGAATTTCCGCTTTTTTCTCTACTTCTATTGGTCAA
AAGCTGCTGAAAGCGGACTGGGTGAAACGAGAAGTGTCATTTAGTATGGCGTTACCTGTTAAAGAGGTATACAGCCATAT
TGATACAGAGGGGGAGCCTGTCCTTATTCAAGGGATGATTGACTGTTTATTTGAAACGGATGGAAAACTATATTTACTAG
ATTATAAAACAGATAGAGTGCAAGGCAGATATACAGGCGGTCTTGAAGCAGCTGTACCTATTTTGAAAAAGCGCTATGAA
ACTCAAATAGCGTTGTATGCACAAGCGGTGGAACGTTTAACAAACAGAACATTAGAGGAGAAAATTCTTTATTTCTTTGA
TGGGAATTTAGAAATTTCATTATAA

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-462)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1052-1226)

UvrD-like helicase C-terminal domain (UvrD_C)

(473-883)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

66.424

100

0.665