Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   QNH25_RS11555 Genome accession   NZ_CP126087
Coordinates   2285160..2286230 (-) Length   356 a.a.
NCBI ID   WP_025092972.1    Uniprot ID   A0A5C0WKU5
Organism   Bacillus safensis strain CEW-AP100     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2280160..2291230
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QNH25_RS11505 (QNH25_11505) - 2280254..2280826 (-) 573 WP_024422971.1 signal peptidase I -
  QNH25_RS11510 (QNH25_11510) tapA 2280879..2281406 (-) 528 WP_133744194.1 amyloid fiber anchoring/assembly protein TapA -
  QNH25_RS11515 (QNH25_11515) - 2281706..2281984 (+) 279 WP_171464609.1 DUF3889 domain-containing protein -
  QNH25_RS11520 (QNH25_11520) - 2282019..2282213 (-) 195 WP_024422974.1 YqzE family protein -
  QNH25_RS11525 (QNH25_11525) comGG 2282273..2282656 (-) 384 WP_234107215.1 competence type IV pilus minor pilin ComGG -
  QNH25_RS11530 (QNH25_11530) comGF 2282653..2283180 (-) 528 WP_267955778.1 competence type IV pilus minor pilin ComGF -
  QNH25_RS11535 (QNH25_11535) comGE 2283092..2283406 (-) 315 WP_283859498.1 competence type IV pilus minor pilin ComGE -
  QNH25_RS11540 (QNH25_11540) comGD 2283390..2283836 (-) 447 WP_024422976.1 competence type IV pilus minor pilin ComGD -
  QNH25_RS11545 (QNH25_11545) comGC 2283829..2284122 (-) 294 WP_024422977.1 competence type IV pilus major pilin ComGC Machinery gene
  QNH25_RS11550 (QNH25_11550) comGB 2284139..2285179 (-) 1041 WP_234107217.1 competence type IV pilus assembly protein ComGB -
  QNH25_RS11555 (QNH25_11555) comGA 2285160..2286230 (-) 1071 WP_025092972.1 competence type IV pilus ATPase ComGA Machinery gene
  QNH25_RS11560 (QNH25_11560) - 2286411..2287286 (-) 876 WP_024422979.1 STAS domain-containing protein -
  QNH25_RS11570 (QNH25_11570) - 2287510..2287890 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  QNH25_RS11575 (QNH25_11575) - 2288103..2288345 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  QNH25_RS11580 (QNH25_11580) - 2288393..2289031 (-) 639 WP_056767335.1 MBL fold metallo-hydrolase -
  QNH25_RS11585 (QNH25_11585) - 2289189..2289362 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  QNH25_RS11590 (QNH25_11590) - 2289388..2289702 (-) 315 WP_024427984.1 MTH1187 family thiamine-binding protein -
  QNH25_RS11595 (QNH25_11595) - 2289779..2291032 (-) 1254 WP_056767338.1 MFS transporter -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40412.86 Da        Isoelectric Point: 9.5360

>NTDB_id=835132 QNH25_RS11555 WP_025092972.1 2285160..2286230(-) (comGA) [Bacillus safensis strain CEW-AP100]
MYGIEYLRQELLEEACRMRASDVHIVPKEKEASVSFRVDSDLIQQRTIDKKSGERLIAHFKFLSSMDIGEKRRPQNGSLA
VMLRKGQVFIRMSTLPTVNDESLVIRILPQDHVPKIKHLSLFPKASARLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKNFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDVIVLGEIRDAETARTAIRAALTGHLVLSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELTCPFCGERCQLYCKLNRPIRRTNVFELLFGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKNTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=835132 QNH25_RS11555 WP_025092972.1 2285160..2286230(-) (comGA) [Bacillus safensis strain CEW-AP100]
TTGTATGGGATTGAATATTTAAGGCAAGAGCTGCTAGAAGAAGCATGCCGCATGAGGGCTTCTGATGTGCATATTGTGCC
AAAAGAAAAGGAAGCTTCGGTGTCATTTCGTGTTGACTCAGATTTAATCCAGCAGCGAACGATTGATAAAAAAAGCGGAG
AGCGGCTTATTGCACATTTTAAGTTTTTATCTTCTATGGATATCGGGGAAAAAAGGAGACCGCAAAATGGCTCACTAGCT
GTGATGCTAAGAAAAGGTCAAGTATTTATTCGGATGTCTACCTTGCCGACAGTGAATGATGAGAGCTTAGTGATTAGAAT
TTTACCGCAGGATCATGTTCCGAAAATAAAACATCTATCATTATTTCCGAAAGCCTCAGCCAGATTATTATCCTTTTTAA
ACCACTCGCATGGACTCATTTTATTTACTGGGCCAACCAATTCGGGGAAAACAACAACCCTCTATTCACTGATCCAGTTT
GCAAAAAAGAATTTCAACCGAAATATTATTACACTTGAAGATCCTGTCGAAACAAGAAATGAAGAAGTATTACAGGTTCA
AGTAAATGAAAAAGCCGGCATCACGTATGCCGCTGGATTACGCGCTATATTAAGACATGATCCAGATGTGATTGTGCTAG
GAGAAATAAGAGATGCTGAAACGGCCAGAACGGCGATTAGAGCAGCACTGACAGGCCATTTAGTATTGAGTACGCTCCAT
GCAAAAAATGCAAAAGGAGCCCTTTACCGCATGCTTGAATTTGGTGTCACGATGAATGAACTTGAGCAAACGATGGTTGC
GATTGCTGCCCAGCGATTAATCGAGCTCACCTGTCCTTTTTGCGGAGAAAGATGTCAGCTTTACTGTAAATTAAATCGAC
CGATCAGACGAACAAATGTATTTGAACTGCTGTTTGGAAAAGAGCTTGGTGAGTGTATCAAGGAGGCTAAAGGAGAATAT
GCTCACTCGTCATATGAAACACTGCAAAGATTAATTCGTAAAGGAGTGGCACTCGGCTATTTATCAAAAAACACTTACCA
TCGCTGGGTTTATGAAGAAGCGAGTCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5C0WKU5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.689

99.438

0.643