Detailed information NTG0835110

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: QNH25_RS05250
• Genome accession: NZ_CP126087
• Coordinates: 1077636..1081340 (+)
• Length: 1234 a.a.
• NCBI ID: WP_283859994.1
• Uniprot ID: -
• Organism: Bacillus safensis strain CEW-AP100
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1072636..1086340

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QNH25_RS05235 (QNH25_05235)	-	1072943..1073353 (+)	411	WP_024425497.1	DoxX family protein	-
QNH25_RS05240 (QNH25_05240)	-	1073550..1073996 (-)	447	WP_024425498.1	ferritin-like domain-containing protein	-
QNH25_RS05245 (QNH25_05245)	addB	1074149..1077658 (+)	3510	WP_283859993.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
QNH25_RS05250 (QNH25_05250)	addA	1077636..1081340 (+)	3705	WP_283859994.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
QNH25_RS05255 (QNH25_05255)	sbcD	1081367..1082542 (+)	1176	WP_283859995.1	exonuclease subunit SbcD	-
QNH25_RS05260 (QNH25_05260)	-	1082539..1085934 (+)	3396	WP_283859996.1	SMC family ATPase	-
QNH25_RS05265 (QNH25_05265)	-	1085955..1086254 (+)	300	WP_024425503.1	hypothetical protein	-

Sequence

Protein

• Length: 1234 a.a.
• Molecular weight: 142255.31 Da
• Isoelectric Point: 5.0351

>NTDB_id=835110 QNH25_RS05250 WP_283859994.1 1077636..1081340(+) (addA) [Bacillus safensis strain CEW-AP100]
MQIPKPNNSTWTDDQWEAIVSEGQDILVAAAAGSGKTAVLVERLIRKMTRPEDPVDVDRLLVVTFTNASAAEMKHRITEA
LEKELAKNPGSLHMRRQLSLMNRANISTLHSFCLQVLRTFYYEIDLDPGFRLADQTEGELLGDEVLDELFEDEYKAGKPA
FFELVDRYTSDRHDLDLQWLVKRIYEFSRSHPSPEQWMRAFLSLYDVEAQTKVEQLPFYPYIKEDLSLVFRSCLELLERA
LTLSKEPGGPAPRAENFIDDLEQVNELIRHQDDFEKLYELLPNINFKRLKTCKGDEYDPVLLEKATDARNQAKKQLEKLK
DEYFMRSPAQHLKSLAEMKPVVETLVELVIQFGQRFERAKQEKSIVDFSDLEHYCLRILAEQDAEGNLIETEAAKYYQQQ
FEEVLVDEYQDTNLVQETILKLVSKGEHAAEGNLFMVGDVKQSIYRFRLAEPMLFLTKYKQFTPDGKETGKRIDLNKNFR
SRSDVLDSTNFLFKQLMGETVGEIEYDEQAELKLGASYPESEDTTTEMLLVHLDQQEAETDEEREELETVQLEARVIAKK
IRQLVEQPFQVYDSKQQMTRNLQYRDIVILLRSMPWAPQMMEELKKQGIPVYANLSSGYFEATEVSVILSLLKVIDNPYQ
DIPLAAVLRSPIVHIDENEMALIRTSDKKGTYYDAVKAFMSGTHSDHPTCKKLERFFRLLRKWRDFSMNHSVAELIWEVY
RDTQYLDYVGGMPGGKQRQANLRALYDRAKQYEKAAFRGLFRFLRFIERMQERGDDLGAAKTFSETEDVVRMMTIHSSKG
LEFPVVFTAGLGRNFNMMDLNQSYLLDKELGFGSKYIHPELRISYATLPLVAMKKKMRKELLSEELRVLYVALTRAKEKL
FLVGSVKNQVKALSKWQNAATGEDWLIPDFERYQSKTYLDFIGPALIRHQAMSSILEEKGDVVLSHPSAFTISFTQASDL
LKENMSLEKKQQDEVVQALMDGLPVEGYGDADEKVAERLSWRYPYLAASQVGTKQSVSEIKRMKEIQDEYSVPSSLRRAH
ATLYDRPAFMKKKALTAAEQGTAMHTVMQHIPLSSDEPLDEIRIHQLLDSLQQRDLLTNEQVQSIDQEGISAFFSTSIGQ
KLLKADWVKREVSFSMALPVKEVYSHIDTEGEPVLIQGMIDCLFETDGKLYLLDYKTDRVQGRYTGGLEAAVPILKKRYE
TQIALYAQAVERLTNRTLEEKILYFFDGNLEISL

Nucleotide

• Length: 3705 bp

>NTDB_id=835110 QNH25_RS05250 WP_283859994.1 1077636..1081340(+) (addA) [Bacillus safensis strain CEW-AP100]
ATGCAAATACCAAAACCGAATAACAGTACTTGGACGGATGACCAGTGGGAAGCCATCGTTTCAGAAGGACAAGATATCCT
TGTTGCGGCGGCGGCAGGGTCAGGTAAAACAGCTGTTTTGGTTGAACGGCTGATTCGAAAAATGACCCGTCCTGAAGATC
CAGTTGATGTGGATCGTCTGCTCGTTGTGACTTTCACAAATGCATCTGCGGCAGAGATGAAGCATCGGATCACAGAAGCA
CTTGAAAAGGAATTAGCCAAAAACCCCGGGTCCCTTCATATGAGAAGACAGCTGTCGCTCATGAATCGTGCCAATATTTC
CACCCTGCACTCCTTTTGCTTGCAGGTTTTGCGCACCTTTTACTATGAGATTGACCTAGATCCAGGCTTTCGTTTGGCTG
ATCAAACAGAAGGAGAATTATTAGGAGATGAAGTGTTAGATGAACTGTTTGAGGATGAATATAAGGCTGGAAAGCCAGCG
TTCTTTGAACTGGTTGATCGTTACACTTCTGATCGTCATGATTTAGACTTGCAATGGCTTGTGAAGAGAATTTATGAGTT
TTCCAGATCTCACCCTTCACCTGAGCAATGGATGAGGGCATTCCTTTCTCTTTATGATGTAGAAGCTCAAACAAAAGTAG
AACAATTACCGTTTTACCCATATATCAAAGAGGACCTTTCTCTCGTTTTTCGAAGCTGTCTGGAACTGCTCGAACGAGCC
CTCACCCTATCAAAAGAACCGGGCGGTCCGGCACCGAGAGCAGAAAATTTTATAGATGATTTAGAGCAAGTCAATGAATT
AATCAGGCACCAAGATGATTTTGAGAAGCTTTACGAGCTTTTGCCAAACATCAATTTTAAAAGGCTCAAAACATGTAAAG
GGGACGAATATGACCCTGTGCTGTTAGAAAAAGCCACAGATGCACGCAATCAAGCAAAAAAACAATTAGAAAAGCTTAAA
GATGAATACTTTATGCGCAGTCCCGCCCAGCATTTGAAAAGCTTAGCTGAAATGAAGCCGGTGGTCGAGACACTTGTGGA
GCTTGTCATCCAATTTGGACAGCGTTTCGAAAGAGCGAAGCAAGAAAAATCAATCGTGGATTTTTCGGATTTAGAGCATT
ATTGCTTACGCATTTTAGCAGAGCAGGACGCGGAAGGAAATTTGATCGAAACAGAAGCGGCCAAGTACTATCAACAGCAG
TTTGAAGAAGTGCTCGTTGATGAATATCAAGATACAAACCTTGTGCAAGAAACGATTTTAAAGCTTGTATCTAAAGGGGA
ACATGCTGCAGAGGGCAACCTGTTTATGGTTGGTGATGTAAAGCAGTCCATATATCGTTTTAGACTGGCTGAGCCCATGC
TCTTTTTAACCAAATATAAACAATTCACACCAGACGGCAAAGAAACAGGGAAGAGAATTGATTTAAATAAAAACTTCCGC
AGCCGTTCAGATGTGTTAGACAGTACGAACTTTTTGTTCAAACAGCTGATGGGGGAAACAGTTGGAGAAATTGAATATGA
TGAACAAGCTGAATTAAAGCTTGGAGCGAGTTATCCAGAAAGCGAAGATACAACGACAGAAATGCTTCTTGTACATTTAG
ATCAACAGGAAGCAGAAACTGACGAAGAGCGAGAAGAGCTAGAAACCGTACAGCTTGAAGCGAGAGTCATTGCTAAAAAA
ATAAGACAATTGGTGGAGCAGCCATTTCAAGTGTATGATTCAAAACAGCAAATGACCCGTAACTTGCAGTATCGGGACAT
TGTGATTTTGCTTCGCTCCATGCCGTGGGCACCGCAAATGATGGAGGAATTGAAGAAGCAGGGAATCCCTGTGTATGCGA
ATCTTTCCTCTGGTTATTTTGAAGCAACAGAGGTGTCTGTGATTCTTTCTCTGTTAAAAGTCATTGATAACCCATATCAA
GATATCCCGCTTGCAGCTGTATTAAGATCACCAATTGTTCATATAGACGAAAATGAAATGGCACTGATCCGCACGAGTGA
TAAAAAAGGCACCTATTATGATGCTGTGAAAGCATTTATGAGTGGGACTCATTCAGATCACCCTACATGCAAAAAACTAG
AACGATTTTTCAGGTTGTTGCGTAAGTGGCGTGATTTCTCTATGAATCATTCAGTAGCGGAATTGATTTGGGAAGTATAC
CGCGATACGCAGTATCTAGATTACGTCGGCGGGATGCCTGGAGGCAAACAGAGACAGGCGAATCTCCGTGCTCTTTATGA
TCGAGCGAAGCAGTATGAAAAAGCTGCTTTTAGAGGGCTGTTCCGTTTTCTTCGCTTTATCGAAAGAATGCAGGAGCGTG
GAGATGATCTTGGGGCGGCAAAAACGTTCAGTGAAACAGAGGACGTTGTTCGCATGATGACGATCCACAGCAGTAAAGGA
TTAGAATTTCCAGTCGTCTTTACCGCAGGACTCGGCAGAAACTTTAATATGATGGACTTAAACCAGTCCTACTTGCTTGA
TAAAGAATTAGGCTTTGGAAGCAAATATATTCATCCAGAGCTGAGAATTAGTTATGCAACATTACCGCTTGTTGCGATGA
AAAAAAAGATGAGGAAAGAGCTATTATCTGAAGAGCTGAGAGTTCTATATGTAGCGCTCACAAGGGCTAAGGAAAAATTA
TTTCTTGTCGGCTCTGTCAAAAATCAGGTGAAGGCATTAAGCAAATGGCAAAATGCTGCAACCGGAGAGGATTGGCTCAT
CCCTGATTTTGAACGTTATCAATCGAAAACATACTTAGACTTTATTGGTCCTGCTCTCATCCGTCACCAAGCAATGTCAT
CTATTTTAGAAGAAAAAGGTGATGTTGTTCTTTCGCACCCATCAGCATTCACAATCTCATTTACTCAAGCTTCCGATCTT
CTAAAAGAAAATATGTCACTTGAAAAGAAGCAGCAAGATGAGGTGGTACAAGCATTGATGGACGGCTTACCTGTTGAAGG
TTATGGTGATGCTGATGAGAAAGTAGCAGAGAGACTTTCATGGAGATACCCGTATCTAGCGGCCTCACAAGTCGGCACAA
AGCAGTCTGTTTCAGAGATAAAGAGAATGAAAGAAATACAGGATGAGTACAGTGTTCCTTCTTCTCTTAGAAGAGCTCAT
GCAACGTTGTATGACCGGCCGGCTTTCATGAAGAAAAAAGCACTCACTGCCGCAGAGCAAGGCACCGCAATGCATACAGT
CATGCAGCATATTCCGCTTTCTTCTGACGAGCCCCTCGATGAAATCCGTATACATCAGCTGCTCGATTCCTTACAGCAGC
GAGACTTGCTAACAAATGAGCAAGTTCAGTCAATTGATCAAGAAGGAATTTCCGCTTTTTTCTCTACTTCTATTGGTCAA
AAGCTGCTGAAAGCGGACTGGGTGAAACGAGAAGTGTCATTTAGTATGGCGTTACCTGTTAAAGAGGTATACAGCCATAT
TGATACAGAGGGGGAGCCTGTCCTTATTCAAGGGATGATTGACTGTTTATTTGAAACGGATGGAAAACTATATTTACTAG
ATTATAAAACAGATAGAGTGCAAGGCAGATATACAGGCGGTCTTGAAGCAGCTGTACCTATTTTGAAAAAGCGCTATGAA
ACTCAAATAGCGTTGTATGCACAAGCGGTGGAACGTTTAACAAACAGAACATTAGAGGAGAAAATTCTTTATTTCTTTGA
TGGGAATTTAGAAATTTCATTATAA

Domains

Predicted by InterproScan.

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (11-462)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1052-1226)

• UvrD-like helicase C-terminal domain (UvrD_C) (473-883)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	66.424	100	0.665