Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ZMTM_RS07360 Genome accession   NZ_AP024110
Coordinates   1517439..1518542 (+) Length   367 a.a.
NCBI ID   WP_221763273.1    Uniprot ID   A0A8D5JR62
Organism   Methyloradius palustris strain Zm11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1512439..1523542
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ZMTM_RS07350 (ZMTM_14080) - 1512647..1514758 (+) 2112 WP_221763271.1 tetratricopeptide repeat protein -
  ZMTM_RS07355 (ZMTM_14090) - 1514762..1517167 (-) 2406 WP_221763272.1 EAL domain-containing protein -
  ZMTM_RS07360 (ZMTM_14100) pilU 1517439..1518542 (+) 1104 WP_221763273.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ZMTM_RS07365 (ZMTM_14110) - 1518615..1518959 (+) 345 WP_221763274.1 DUF805 domain-containing protein -
  ZMTM_RS07370 (ZMTM_14120) - 1519006..1519890 (-) 885 WP_221763275.1 DMT family transporter -
  ZMTM_RS07375 (ZMTM_14130) dapC 1520005..1521195 (+) 1191 WP_221765630.1 succinyldiaminopimelate transaminase -
  ZMTM_RS07380 (ZMTM_14140) - 1521344..1522288 (+) 945 WP_221763276.1 IS1595 family transposase -
  ZMTM_RS07385 (ZMTM_14150) - 1522278..1522448 (+) 171 WP_221763277.1 hypothetical protein -
  ZMTM_RS07390 (ZMTM_14160) - 1522583..1522984 (-) 402 WP_221763278.1 BlaI/MecI/CopY family transcriptional regulator -
  ZMTM_RS07395 (ZMTM_14170) - 1523045..1523476 (-) 432 WP_221763279.1 hypothetical protein -

Sequence

Protein


Download         Length: 367 a.a.        Molecular weight: 40182.42 Da        Isoelectric Point: 6.1812

>NTDB_id=83488 ZMTM_RS07360 WP_221763273.1 1517439..1518542(+) (pilU) [Methyloradius palustris strain Zm11]
MAAIDITPVLKFMVEKNGSDLFFSTGAAIHIDIEGKTLAVNNQVMSAGMVKEIAYALMSPAQIQEFETTLEMNFAYSVAD
IDEARFRVNIFRQRGDVAMVIRCIKAIIPSFETLGLPNILKDLISRQRGLILVVGSTGSGKSTTLASMIDHRNETTTGHI
LTLEDPIEFIHKHKKSIVDQREIGIDTHSFDNGLKNAMRQSPDVILIGEIRDMESMKHAMTYSETGHLCLATLHANNANQ
AIERIISFFPPEGKAGLLLGLSMNLISVVSQRLVVGIHPKRAAAVEVLINTPYISELILKQKLDEIKEVMADSNDIGMCT
FDQSLFRLFSNGKITEESALAAADSRNDLSLKMRFAAEGAKGGGFGA

Nucleotide


Download         Length: 1104 bp        

>NTDB_id=83488 ZMTM_RS07360 WP_221763273.1 1517439..1518542(+) (pilU) [Methyloradius palustris strain Zm11]
ATGGCTGCGATAGACATCACCCCCGTACTCAAATTTATGGTGGAGAAAAATGGTTCTGACCTGTTTTTTAGTACAGGCGC
TGCCATCCACATTGATATTGAGGGTAAAACACTCGCAGTCAATAACCAAGTGATGAGCGCTGGCATGGTTAAGGAAATCG
CCTATGCGCTGATGAGCCCTGCACAAATACAGGAGTTTGAAACCACGCTAGAAATGAATTTTGCCTACAGCGTGGCTGAT
ATTGATGAAGCGCGCTTTCGCGTGAATATCTTCCGCCAGCGTGGTGATGTGGCGATGGTTATTCGCTGTATCAAGGCCAT
CATTCCATCCTTTGAAACACTTGGCCTGCCCAATATTCTTAAAGACTTAATTTCACGTCAGCGCGGGCTTATTCTGGTGG
TAGGCTCTACTGGGTCGGGTAAGTCAACCACACTCGCCTCGATGATTGATCATCGCAACGAAACGACCACAGGGCATATT
CTTACGCTTGAGGACCCGATTGAGTTCATCCACAAACATAAAAAATCTATCGTGGATCAACGTGAAATCGGTATCGACAC
TCACTCTTTTGATAATGGCCTGAAGAATGCCATGCGCCAATCACCTGATGTGATTCTGATTGGAGAAATCCGTGATATGG
AGAGCATGAAACATGCAATGACATACTCGGAAACGGGGCATTTATGCCTTGCCACATTGCACGCCAACAATGCCAACCAG
GCGATTGAACGCATCATTTCGTTCTTCCCGCCTGAAGGTAAAGCAGGTTTATTGCTGGGCTTGTCTATGAACCTCATCTC
GGTCGTATCGCAAAGGCTGGTCGTGGGCATACACCCAAAACGCGCGGCGGCGGTTGAAGTGCTGATCAACACCCCTTACA
TTTCAGAATTAATACTCAAACAAAAATTAGATGAAATCAAAGAAGTAATGGCAGACAGCAATGACATTGGCATGTGTACG
TTTGACCAGTCGCTGTTCAGGTTATTCAGCAACGGTAAGATCACTGAAGAAAGTGCGCTGGCTGCCGCTGATTCACGTAA
TGATCTATCACTCAAAATGAGGTTTGCTGCAGAAGGGGCAAAAGGTGGTGGCTTCGGTGCATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(15-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.567

95.64

0.493
  pilU Vibrio cholerae strain A1552

47.671

99.455

0.474
  pilU Acinetobacter baylyi ADP1

47.778

98.093

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.566

93.46

0.398
  pilT Legionella pneumophila strain Lp02

42.647

92.643

0.395
  pilT Legionella pneumophila strain ERS1305867

42.647

92.643

0.395
  pilT Acinetobacter nosocomialis M2

41.471

92.643

0.384
  pilT Acinetobacter baylyi ADP1

41.228

93.188

0.384
  pilT Neisseria gonorrhoeae MS11

40

95.368

0.381
  pilT Neisseria meningitidis 8013

40

95.368

0.381
  pilT Acinetobacter baumannii strain A118

41.176

92.643

0.381
  pilT Acinetobacter baumannii D1279779

41.176

92.643

0.381
  pilT Pseudomonas aeruginosa PAK

40.882

92.643

0.379
  pilT Pseudomonas stutzeri DSM 10701

40.179

91.553

0.368

Multiple sequence alignment