Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ZMTM_RS00925 Genome accession   NZ_AP024110
Coordinates   187010..188452 (-) Length   480 a.a.
NCBI ID   WP_221764489.1    Uniprot ID   -
Organism   Methyloradius palustris strain Zm11     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 182010..193452
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ZMTM_RS00895 (ZMTM_01680) ptsP 182206..183951 (-) 1746 WP_221764483.1 phosphoenolpyruvate--protein phosphotransferase -
  ZMTM_RS00900 (ZMTM_01690) - 183984..184256 (-) 273 WP_221764484.1 HPr family phosphocarrier protein -
  ZMTM_RS00905 (ZMTM_01700) - 184253..184657 (-) 405 WP_221764485.1 PTS sugar transporter subunit IIA -
  ZMTM_RS00910 (ZMTM_01710) rapZ 184704..185534 (-) 831 WP_221764486.1 RNase adapter RapZ -
  ZMTM_RS00915 (ZMTM_01720) hprK 185538..186500 (-) 963 WP_221764487.1 HPr(Ser) kinase/phosphatase -
  ZMTM_RS00920 (ZMTM_01730) hpf 186651..186977 (-) 327 WP_221764488.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  ZMTM_RS00925 (ZMTM_01740) rpoN/rpoN1 187010..188452 (-) 1443 WP_221764489.1 RNA polymerase factor sigma-54 Machinery gene
  ZMTM_RS00930 (ZMTM_01750) lptB 188476..189198 (-) 723 WP_221764490.1 LPS export ABC transporter ATP-binding protein -
  ZMTM_RS00935 (ZMTM_01760) lptA 189247..189789 (-) 543 WP_221764491.1 lipopolysaccharide transport periplasmic protein LptA -
  ZMTM_RS00940 (ZMTM_01770) lptC 189770..190570 (-) 801 WP_225907122.1 LPS export ABC transporter periplasmic protein LptC -
  ZMTM_RS00945 (ZMTM_01780) - 190612..191139 (-) 528 WP_221764493.1 HAD family hydrolase -
  ZMTM_RS00950 (ZMTM_01790) - 191143..192141 (-) 999 WP_221764494.1 KpsF/GutQ family sugar-phosphate isomerase -

Sequence

Protein


Download         Length: 480 a.a.        Molecular weight: 54588.04 Da        Isoelectric Point: 4.9460

>NTDB_id=83481 ZMTM_RS00925 WP_221764489.1 187010..188452(-) (rpoN/rpoN1) [Methyloradius palustris strain Zm11]
MKQSLQLKFSQHLALTPQLQQSIRLLQLSTQELNQELETLLQENPLLERTTNTDQPVDLEAEAFVPNNTVQETSIPATDE
PARQEEYGDDYFEFSSGNRWEDNSAGNDDENDFSFQESVSLTLREHLISQLKLMQFSERDQTLAMLLVDTINDDGYLEQP
LEELLELLPDELEIDLLELQTALKHIQHLDPPGVGARSLAECLALQLQAMPEDTPHLGLAINVALQHLPILASRDYAKLR
KTLQCDDDVLRAVQLMVTQLNPRPGSDFTKIGSDHYIQHEILVKKVKGLWIATLNDEVVPRLRINQLYAGILKRNRDSSS
QYLMSQMQEAKWMIKNIQQRFSTILRVSQAIVDRQRNFFEHGEVAMRPLVLREIADELGLHESTVSRVTTRKYMLTPRGV
YELKYFFGSHVSTESGGACSATAIRALIKQMVSEENAKKPLSDNQITDILSKQGIMVARRTIAKYRESLSIPAANQRKSI

Nucleotide


Download         Length: 1443 bp        

>NTDB_id=83481 ZMTM_RS00925 WP_221764489.1 187010..188452(-) (rpoN/rpoN1) [Methyloradius palustris strain Zm11]
ATGAAACAAAGCCTACAACTTAAATTTTCGCAACATTTGGCGCTCACACCGCAGTTGCAGCAGTCTATTCGCTTGCTTCA
GCTATCAACTCAAGAGTTGAATCAAGAGCTAGAAACCTTGCTGCAAGAAAACCCCCTGCTTGAACGCACCACCAATACTG
ACCAGCCTGTTGATCTGGAAGCAGAAGCTTTTGTGCCAAACAACACGGTGCAGGAAACATCGATTCCAGCCACTGATGAG
CCCGCACGTCAGGAAGAATACGGCGATGACTATTTTGAGTTTTCCAGCGGTAATCGTTGGGAAGACAACAGCGCAGGCAA
TGACGACGAAAACGACTTTAGCTTTCAGGAGTCTGTCTCTCTTACCTTACGTGAACACCTGATCAGCCAGCTTAAATTGA
TGCAATTCTCAGAGCGGGATCAAACGCTCGCCATGTTGCTGGTTGATACCATTAATGATGATGGCTATCTTGAACAGCCA
TTAGAAGAACTACTGGAATTACTGCCTGATGAGCTCGAAATTGATCTGCTAGAGCTACAAACCGCGCTCAAACACATCCA
GCATTTAGATCCTCCTGGCGTAGGCGCCCGTAGCCTAGCCGAATGCCTGGCATTACAACTGCAAGCAATGCCAGAAGATA
CGCCGCATTTGGGCTTGGCGATCAATGTAGCACTCCAACACTTACCCATACTGGCCAGCAGAGACTACGCGAAGCTGCGT
AAAACCCTGCAATGCGATGACGATGTATTACGCGCAGTTCAGCTCATGGTCACGCAGCTCAACCCTCGCCCTGGTAGCGA
TTTTACAAAAATCGGCTCCGACCACTATATTCAGCATGAAATTCTGGTCAAAAAAGTCAAAGGCTTATGGATCGCCACGT
TAAATGATGAAGTAGTGCCTAGGCTACGTATCAACCAACTTTATGCAGGTATTTTAAAGCGCAATCGTGACAGCTCCAGC
CAATACCTGATGAGCCAGATGCAAGAAGCGAAATGGATGATCAAGAATATCCAGCAGCGCTTCTCGACGATTTTGCGGGT
CTCACAAGCGATTGTAGATAGACAACGTAACTTTTTCGAGCATGGTGAAGTCGCTATGCGCCCGCTGGTTTTGCGTGAAA
TCGCTGATGAACTGGGTTTACATGAATCCACCGTCTCTCGCGTCACCACACGAAAATATATGCTTACACCACGTGGTGTG
TATGAGCTTAAATATTTCTTCGGTAGCCATGTATCTACCGAATCTGGCGGCGCTTGCTCTGCCACTGCGATACGCGCATT
GATCAAGCAAATGGTGAGCGAAGAAAATGCGAAAAAACCCTTATCAGACAACCAGATTACTGATATTTTGAGTAAGCAAG
GAATTATGGTGGCACGACGCACCATTGCAAAATACCGCGAGTCTCTCTCGATACCCGCGGCTAACCAGCGCAAATCCATT
TAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(120-308)

Sigma-54, DNA binding domain (Sigma54_DBD)

(321-478)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

51.287

100

0.54

Multiple sequence alignment