Detailed information NTG0834743

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: QNH35_RS05435
• Genome accession: NZ_CP126081
• Coordinates: 1109990..1113688 (+)
• Length: 1232 a.a.
• NCBI ID: WP_283924311.1
• Uniprot ID: -
• Organism: Bacillus pumilus strain B2_10
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1104990..1118688

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QNH35_RS05420 (QNH35_05420)	-	1105285..1105695 (+)	411	WP_065098902.1	DoxX family protein	-
QNH35_RS05425 (QNH35_05425)	-	1105892..1106338 (-)	447	WP_283924309.1	ferritin-like domain-containing protein	-
QNH35_RS05430 (QNH35_05430)	addB	1106503..1110012 (+)	3510	WP_283924310.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
QNH35_RS05435 (QNH35_05435)	addA	1109990..1113688 (+)	3699	WP_283924311.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
QNH35_RS05440 (QNH35_05440)	sbcD	1113715..1114890 (+)	1176	WP_106064954.1	exonuclease subunit SbcD	-
QNH35_RS05445 (QNH35_05445)	-	1114887..1118282 (+)	3396	WP_283924312.1	SMC family ATPase	-
QNH35_RS05450 (QNH35_05450)	-	1118302..1118601 (+)	300	WP_065098897.1	hypothetical protein	-

Sequence

Protein

• Length: 1232 a.a.
• Molecular weight: 142143.17 Da
• Isoelectric Point: 5.1200

>NTDB_id=834743 QNH35_RS05435 WP_283924311.1 1109990..1113688(+) (addA) [Bacillus pumilus strain B2_10]
MQIPKPNNSTWTDDQWEAIVSEGQDILVAAAAGSGKTAVLVERLIRKMTRPEHPVDVDRLLVVTFTNASAAEMKHRITEA
LEKELAKNPGSLHMRRQLSLMNRANISTLHSFCLQVLRTFYYEIDLDPGFRLADQTEGELLGDEVLDELFEDEYKAGKPS
FFELVDRYTSDRHDLDLQWLVKRIYEFSRSHPSPEQWMRAFLSLYDVDAQTKVEELPFYPYIKEDLSLVLRSCQELLERA
LSLSKEPSGPAPRAENFIDDLEQVNELIRHQDDFEKLYELLPNVNFKRLKTCKGDEYDPALIEKATDARNQAKKQLEKLK
DEYFMRSPAQHLKSLAEMKQVVETLVELVIQFGERFERAKQEKSIVDFSDLEHYCLRILAEQDAEGHLIETEAAKYYQQQ
FEEVLVDEYQDTNLVQETILKLVSKGKHSAEGNLFMVGDVKQSIYRFRLAEPMLFLNKYKHFQPDGKETGKRIDLNKNFR
SRSDVLDSTNFLFKQLMGGTVGEIEYDEQAELKLGASYPESKDTTTEMLLVHLDQQETEPGEEREELETVQFEARIIAKK
IKELVEQPFQVYDAKQQMTRNLQYRDIVILLRSMPWAPQMMEELKKQGIPVYANLSSGYFEATEVSVILSLLKVIDNPYQ
DIPLAAVLRSPIVHLDENELALIRTSDKKGTYYDAVKAFMSVTHSDHPTCKKLERFFQLLRKWRDFSINHSVAELIWEVY
RDTQYLDYVGGMPGGKQRKANLRALYDRAKQYEKAAFRGLFRFLRFIERMQERGDDLGAAKTFSETEDVVRMMTIHSSKG
LEFPVVFTAGLGRNFNMMDLNQSYLLDKELGFGSKYIHPELRISYATLPLVAMKKKMRKELLSEELRVLYVALTRAKEKL
FLVGSVKNQVRALSKWQNAATGEEWLLPDFERYQSKTYLDFIGPALIRHQAMASVLEETGDVVLSHPSTFTISFTQASDL
LKEDMSLEKKEQDEIVQALIDGLPVEGYGDADEQVAERLSWKYPYLAASQVGTKQSVSEIKRMKEIQDEYSVPSSIRKAR
ATLYDRPAFMKKKTLTAAEQGTAMHTVMQHIPLPSEEPYDESRIGHLLESLQQRDLLTDEQIQSINQEGIAAFFSTSIGQ
KLLKADWVKREVSFSMVLPVKEVYSHIDTEGEPVLIQGMIDCLFETDGKLYLLDYKTDRVQGRYTVDAAEPILKKRYETQ
IALYAKAVERLTNRTLEEKILYFFDGNLEISL

Nucleotide

• Length: 3699 bp

>NTDB_id=834743 QNH35_RS05435 WP_283924311.1 1109990..1113688(+) (addA) [Bacillus pumilus strain B2_10]
ATGCAAATACCAAAACCGAATAACAGTACGTGGACGGATGACCAGTGGGAAGCCATCGTTTCAGAAGGACAAGATATCTT
GGTTGCGGCGGCGGCTGGGTCAGGTAAAACAGCTGTTTTGGTTGAACGCCTGATTCGAAAAATGACCCGGCCTGAACATC
CAGTTGATGTGGACCGTCTGCTCGTTGTGACTTTTACAAATGCTTCTGCGGCAGAGATGAAGCATCGGATCACAGAAGCA
CTTGAAAAAGAATTAGCCAAAAACCCTGGGTCTCTTCATATGAGAAGGCAGCTGTCCCTCATGAATCGAGCCAATATTTC
AACCTTGCACTCCTTTTGCTTGCAGGTTTTGCGCACCTTTTACTATGAAATTGATCTTGATCCAGGCTTTCGTTTGGCTG
ACCAAACAGAAGGAGAACTATTAGGAGATGAAGTGTTAGACGAACTGTTTGAGGATGAATATAAGGCTGGAAAGCCATCA
TTCTTTGAATTGGTTGATCGTTACACGTCTGACCGTCACGACTTAGATTTGCAATGGCTCGTGAAGAGAATTTATGAGTT
TTCGAGATCACATCCTTCTCCGGAGCAATGGATGCGGGCATTTCTTTCACTTTATGATGTAGATGCTCAAACAAAAGTAG
AAGAATTACCGTTTTATCCATATATCAAAGAAGATCTTTCTCTCGTTCTCCGGAGCTGCCAGGAGCTGCTTGAACGAGCT
CTTTCGCTATCAAAAGAACCGAGCGGCCCAGCACCGAGAGCAGAGAATTTTATAGATGATTTAGAGCAAGTTAATGAATT
AATCCGTCATCAAGATGATTTTGAGAAACTTTATGAGCTTTTGCCGAACGTCAATTTTAAAAGGCTCAAAACGTGCAAAG
GGGACGAATATGACCCTGCTTTAATAGAAAAAGCCACAGATGCACGTAATCAAGCAAAAAAACAATTAGAAAAACTAAAA
GATGAATATTTCATGCGCAGTCCTGCCCAGCATTTAAAAAGCTTAGCTGAAATGAAGCAAGTTGTGGAGACACTTGTAGA
GCTTGTGATCCAATTTGGTGAGCGTTTCGAAAGAGCGAAGCAGGAAAAGTCAATTGTCGACTTTTCGGATTTAGAGCACT
ATTGCTTACGCATTTTAGCGGAGCAGGATGCAGAAGGACATTTGATCGAAACAGAAGCTGCCAAGTACTATCAACAGCAG
TTTGAAGAAGTACTCGTTGATGAATACCAGGATACAAACCTTGTACAAGAAACGATTTTAAAGCTTGTATCTAAGGGGAA
ACATTCTGCAGAGGGCAATCTGTTTATGGTTGGTGATGTAAAGCAGTCTATTTATCGTTTTAGACTGGCTGAGCCCATGC
TTTTTTTAAACAAATATAAACACTTTCAACCAGACGGCAAAGAAACAGGGAAGAGGATCGATTTAAATAAAAACTTCCGC
AGCCGTTCAGATGTTTTAGATAGTACGAACTTTTTGTTCAAGCAGCTGATGGGGGGAACAGTTGGAGAAATTGAATATGA
TGAACAAGCTGAATTAAAGCTTGGAGCGAGTTATCCAGAAAGCAAAGATACAACGACAGAAATGCTTCTTGTCCACTTAG
ATCAGCAGGAAACAGAACCCGGCGAAGAGCGAGAAGAGCTCGAGACCGTCCAATTTGAAGCGAGAATCATTGCTAAAAAA
ATAAAAGAATTAGTGGAGCAGCCATTTCAAGTGTACGATGCAAAGCAGCAAATGACCCGTAACTTACAGTATCGTGACAT
TGTCATTTTGCTTCGATCTATGCCTTGGGCTCCGCAAATGATGGAGGAATTAAAGAAGCAGGGAATCCCTGTATATGCGA
ATCTTTCCTCTGGTTATTTTGAAGCAACAGAGGTGTCTGTCATTCTTTCTTTGTTAAAAGTCATCGATAATCCATATCAG
GATATCCCGCTTGCTGCTGTTTTAAGATCACCTATTGTTCATTTAGACGAAAATGAATTGGCACTGATTCGAACAAGTGA
TAAAAAAGGCACGTATTATGATGCTGTAAAGGCATTTATGAGTGTGACGCATTCTGATCACCCTACATGCAAAAAACTAG
AGCGATTTTTCCAATTACTGCGCAAGTGGCGTGATTTTTCTATCAATCATTCGGTGGCAGAATTAATTTGGGAAGTCTAC
CGTGACACGCAGTATCTTGATTATGTTGGCGGGATGCCTGGAGGTAAGCAGCGGAAGGCGAATCTTCGTGCCCTTTATGA
CCGGGCCAAGCAATATGAAAAAGCTGCCTTTAGAGGGCTATTTCGATTCCTTCGTTTTATTGAAAGAATGCAGGAGCGTG
GGGATGATCTTGGTGCGGCAAAAACGTTCAGTGAAACAGAGGACGTTGTGCGCATGATGACGATCCATAGCAGTAAAGGA
TTAGAATTTCCAGTCGTCTTTACCGCAGGGCTCGGCAGGAACTTTAATATGATGGACTTAAACCAGTCTTATTTACTTGA
TAAAGAACTAGGCTTTGGAAGCAAGTATATCCATCCTGAGCTGAGAATTAGTTATGCGACATTACCGCTTGTGGCGATGA
AAAAAAAGATGAGAAAAGAGTTATTATCTGAAGAATTAAGGGTCCTATATGTAGCGCTCACAAGGGCTAAAGAAAAATTA
TTTCTGGTCGGCTCTGTCAAAAATCAGGTGAGAGCATTAAGCAAATGGCAAAATGCCGCAACCGGAGAAGAGTGGCTGCT
CCCTGATTTTGAACGGTATCAATCGAAAACATACTTAGATTTCATTGGCCCTGCCCTCATCCGTCATCAAGCGATGGCAT
CTGTTTTAGAAGAAACAGGAGATGTTGTTCTTTCGCATCCGTCTACATTCACTATCTCATTTACGCAAGCTTCTGATCTT
TTAAAGGAAGATATGTCACTTGAAAAGAAGGAGCAAGACGAGATTGTACAAGCATTGATCGATGGTCTGCCTGTTGAAGG
ATATGGTGATGCTGATGAGCAGGTGGCAGAGAGGCTCTCATGGAAATATCCGTATCTAGCGGCCTCGCAAGTCGGCACAA
AGCAGTCTGTTTCCGAGATAAAAAGAATGAAAGAAATACAGGATGAGTACAGTGTTCCTTCTTCTATTCGAAAAGCTCGT
GCAACATTGTATGACCGGCCGGCTTTCATGAAGAAAAAAACACTCACTGCCGCAGAACAAGGCACCGCAATGCATACAGT
GATGCAGCATATCCCGCTTCCTTCTGAGGAGCCCTATGATGAATCACGTATAGGACACTTGCTCGAATCCTTACAGCAGC
GAGACTTGCTAACAGATGAGCAGATCCAGTCAATAAATCAAGAGGGAATTGCTGCTTTCTTCTCTACTTCAATTGGTCAA
AAACTGCTAAAAGCGGACTGGGTGAAACGAGAAGTGTCATTTAGTATGGTGTTACCTGTTAAAGAGGTATACAGTCATAT
TGATACGGAGGGAGAGCCTGTTCTGATTCAAGGGATGATTGACTGTTTATTTGAAACAGATGGAAAGCTATATTTACTAG
ATTATAAAACTGATAGAGTACAAGGCAGATATACAGTTGACGCTGCTGAACCGATCTTGAAAAAGCGTTATGAAACCCAA
ATAGCGTTATATGCAAAAGCTGTAGAACGTTTGACAAACAGAACATTAGAGGAGAAAATTCTCTATTTCTTCGATGGGAA
TTTAGAAATTTCTTTATAA

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (473-883)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1052-1224)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (11-463)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	66.1	100	0.663