Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   QM317_RS13485 Genome accession   NZ_CP125861
Coordinates   2753635..2754705 (+) Length   356 a.a.
NCBI ID   WP_000776406.1    Uniprot ID   -
Organism   Staphylococcus aureus strain 12-03119     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2748635..2759705
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QM317_RS13455 (QM317_13440) - 2749058..2750521 (+) 1464 WP_001017867.1 rhomboid family intramembrane serine protease -
  QM317_RS13460 (QM317_13445) - 2750502..2750705 (+) 204 WP_000087561.1 YqgQ family protein -
  QM317_RS13465 (QM317_13450) - 2750702..2751688 (+) 987 WP_000161314.1 ROK family glucokinase -
  QM317_RS13470 (QM317_13455) - 2751688..2752017 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  QM317_RS13475 (QM317_13460) - 2752014..2752637 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  QM317_RS13480 (QM317_13465) comGA 2752689..2753663 (+) 975 WP_000697227.1 competence type IV pilus ATPase ComGA Machinery gene
  QM317_RS13485 (QM317_13470) comGB 2753635..2754705 (+) 1071 WP_000776406.1 competence type IV pilus assembly protein ComGB Machinery gene
  QM317_RS13490 (QM317_13475) comGC 2754719..2755030 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  QM317_RS13495 (QM317_13480) comGD 2755008..2755454 (+) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  QM317_RS13500 (QM317_13485) comGE 2755441..2755740 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  QM317_RS13505 (QM317_13490) comGF 2755658..2756155 (+) 498 WP_001829055.1 competence type IV pilus minor pilin ComGF Machinery gene
  QM317_RS13510 (QM317_13495) - 2756252..2756398 (+) 147 WP_001789879.1 hypothetical protein -
  QM317_RS13515 (QM317_13500) - 2756388..2756912 (+) 525 WP_001015121.1 shikimate kinase -
  QM317_RS13520 (QM317_13505) gcvT 2757071..2758162 (+) 1092 WP_000093346.1 glycine cleavage system aminomethyltransferase GcvT -
  QM317_RS13525 (QM317_13510) gcvPA 2758182..2759528 (+) 1347 WP_000019683.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 41387.79 Da        Isoelectric Point: 10.0606

>NTDB_id=833080 QM317_RS13485 WP_000776406.1 2753635..2754705(+) (comGB) [Staphylococcus aureus strain 12-03119]
MKLQWINTFKLHSKKRQLSKAQQIDLLSNLCNLLKYGFTLYQSFQFLNLQMAYKNKQLGATILSEISNGAPCNQILSLIG
YSDTIVMQVYLAERFGNIIDVLEETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
SFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKLIYNNLNMLNKINFVMKLPLISGYFQLFKTYFVTNELVLFYKNGITLQ
SIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQILEKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIK
QTQFLQPILFLILGLFIVAIYLVIMLPMFQMMQSIK

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=833080 QM317_RS13485 WP_000776406.1 2753635..2754705(+) (comGB) [Staphylococcus aureus strain 12-03119]
GTGAAACTACAATGGATAAATACATTTAAACTACATTCTAAGAAGCGACAATTAAGTAAGGCCCAACAAATCGACTTACT
TTCAAATTTATGTAATTTGTTGAAATATGGTTTCACTCTGTATCAAAGTTTTCAATTTTTAAATCTTCAAATGGCATATA
AAAATAAGCAATTAGGTGCCACCATTCTAAGTGAAATTTCAAATGGTGCACCATGCAATCAGATATTATCACTGATAGGT
TATAGCGATACTATCGTCATGCAAGTATATTTGGCAGAAAGATTTGGTAATATTATAGACGTTCTAGAAGAAACCGTAAA
TTATATGAAAGTGAATAGAAAGTCAGAACAACGATTGTTAAAGACACTGCAATACCCCTTAATACTAGTTTCTATTTTTA
TTGCTATGATTATTATATTAAACCTCACAGTAATTCCACAGTTTCAACAATTATATACTTCTATGAATATTCAACTATCC
TCTTTTCAAAAAACATTGTCTTTTTTCATTACCAGCTTACCTACTATAATTGTAGTAATGCTCATAATAGTATCTATGTT
GGCTATTATTATGAAATTAATTTATAACAATTTAAATATGCTCAATAAGATAAACTTTGTGATGAAACTACCGCTAATAT
CAGGCTATTTCCAATTATTTAAAACTTATTTTGTAACTAATGAATTAGTGTTGTTTTATAAAAATGGTATTACACTTCAA
TCAATAGTAGACGTTTATATTAACCATAGTAGTGATCCATTTAGACAGTTTCTAGGTAAATACTTATTAACTTATTCAGA
AATGGGATATGGTTTGCCTCAAATTTTAGAAAAACTAAAATGCTTTAAGCCTCAATTAATTAAGTTTGTGCTACAAGGTG
AAAAGAGAGGGAAGCTAGAAGTAGAACTAAAGTTATATTCGCAAATATTAGTAAAACAAATAGAAGATAAAGCGATAAAA
CAGACTCAGTTTTTACAGCCTATTTTATTTTTGATTTTAGGTTTATTTATTGTCGCAATTTATTTAGTAATTATGTTACC
AATGTTTCAAATGATGCAAAGTATAAAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(227-347)

Type II secretion system (T2SS), protein F (T2SSF)

(27-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Staphylococcus aureus MW2

99.438

100

0.994
  comGB Staphylococcus aureus N315

99.438

100

0.994