Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   QMY43_RS00040 Genome accession   NZ_CP125856
Coordinates   8981..9574 (+) Length   197 a.a.
NCBI ID   WP_005480482.1    Uniprot ID   Q87L64
Organism   Vibrio parahaemolyticus strain G855     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3981..14574
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMY43_RS00025 (QMY43_00025) - 4697..7267 (-) 2571 WP_005480476.1 PBP1A family penicillin-binding protein -
  QMY43_RS00030 (QMY43_00030) pilM 7410..8426 (+) 1017 WP_017449463.1 type IV pilus assembly protein PilM Machinery gene
  QMY43_RS00035 (QMY43_00035) pilN 8410..8988 (+) 579 WP_005480484.1 PilN domain-containing protein Machinery gene
  QMY43_RS00040 (QMY43_00040) pilO 8981..9574 (+) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  QMY43_RS00045 (QMY43_00045) pilP 9564..10079 (+) 516 WP_159404023.1 pilus assembly protein PilP Machinery gene
  QMY43_RS00050 (QMY43_00050) pilQ 10111..11859 (+) 1749 WP_005488227.1 type IV pilus secretin PilQ family protein Machinery gene
  QMY43_RS00055 (QMY43_00055) aroK 12048..12566 (+) 519 WP_005381319.1 shikimate kinase AroK -
  QMY43_RS00060 (QMY43_00060) aroB 12598..13698 (+) 1101 WP_005480443.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23091.63 Da        Isoelectric Point: 4.8225

>NTDB_id=832994 QMY43_RS00040 WP_005480482.1 8981..9574(+) (pilO) [Vibrio parahaemolyticus strain G855]
MVNWQDLEMDEIAEWPLLPQLVVICLLVLLIQGFGYWFYLKPEEARLNNLIQEEQTLKVALRIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSENFLYRLPLNIELTGDFHDIGRFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEEKKDEK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=832994 QMY43_RS00040 WP_005480482.1 8981..9574(+) (pilO) [Vibrio parahaemolyticus strain G855]
ATGGTTAATTGGCAAGATCTAGAAATGGATGAGATTGCTGAATGGCCATTACTGCCTCAGCTTGTAGTGATTTGCCTGCT
TGTTCTTCTTATCCAAGGGTTTGGTTATTGGTTTTACTTAAAGCCAGAAGAGGCTCGCTTGAACAATTTGATTCAAGAGG
AGCAAACATTAAAAGTGGCATTGCGCATAAAAGCCAACAAGGTGGCTGCATTGCCTCAATTGCAATCTCAACTGGACGAG
CTCACTACGCGTTATGAGTTGCTGTTGCAACAACTACCGGCACAAAAAGAGTTGGCCACAATGTTGGCGTCGGTAAACGA
ACTCGGTATCGACAACAAGTTAACGTTTACTCGAATCGATTGGGGAGAAAAGCAATCTGAAAACTTCCTGTATCGATTGC
CACTAAATATCGAGCTAACAGGTGATTTCCACGATATCGGTCGTTTTTCACAGGCCATTGCCACACTGCCACGAATCATC
ACCTTTAAAGATGTGACTTGGCAGCGTGTCAGCCAAGAGAGTGAAACTTTGCACTTCCGAGTGAGAGCAAACACTTATCA
GTTCAAGCCTGAGGAAAAGAAAGATGAGAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q87L64

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

94.211

96.447

0.909
  pilO Vibrio cholerae strain A1552

72.589

100

0.726